Alternative Names

GDF-15, MIC-1, NAG-1, PDF, PLAB, PTGFB, GDF15, MIC1, RG-1, Placental TGF-beta, PTGF-beta, PTGFBPTGF-beta, Placental TGF-β, PTGF-β, PTGFBPTGF-β

Species

Human

Expression Sequence

Ala197-Ile308 (H202D)

Accession

Q99988

Tag

N-hFc

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 37.88 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】 Immobilized Human GFRAL, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GDF15 (H202D), hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.

Reconstitution Method

Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.