Alternative Names

Integrin alpha 5 beta 1, VLA-5, ITGA5&ITGB1 Integrin alpha 5 β 1, α5β1

Species

Human

Expression Sequence

Phe42-Tyr995(ITGA5) acidic tail&Gln21-Asp728(ITGB1) basic tail

Accession

P08648(ITGA5)&P05556-1(ITGB1)

Tag

C-His-Avi

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 111.8 kDa (ITGA5)&83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 110-140 kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE,> 90% as determined by HPLC

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】 Immobilized 0.5μg Anti-ITGA5&ITGB1 Antibody, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human ITGA5&ITGB1, His Tag with the EC50 of 55.3ng/ml determined by ELISA.

Reconstitution Method

Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.