Property Name

Property Details

Alternative Names

Ksp-cadherin, CDH16, Cadherin-16

Species

Human

Expression Sequence

Lys19-Ala786

Accession

O75309-1

Tag

N-His

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of MW 84.37 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on SDS-PAGE result.

Purity

>95% as determined by SDS-PAGE and HPLC.

Endotoxin

<1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22 μm filtered solution in 50mM Tris, 100mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】Immobilized Human CDH16, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CDH16 Antibody, Mouse IgG Tag with the EC50 of 7.5ng/ml determined by ELISA.

Reconstitution Method

Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.