Synonyms

PC9; HCHOLA3; FH3; NARC-1

Source

Mouse PCSK9 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln35-Gln694.[Accession | Q80W65]

Molecular Weight

Due to autocatalytic cleavage, the protein release the pro-form (59 kDa) and mature form (14 kDa). Due to glycosylation, the protein migrates to 65-68 kDa (pro-form) and 15kDa (mature form) based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Activity

SPR Data: Human LDLR, His Tag immobilized on CM5 Chip can bind Mouse PCSK9, His Tag with an affinity constant of 0.61 nM as determined in SPR assay .

Formulation

Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCL, 200mM L-Aginine (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.