Synonyms

Hemopexin; FLJ56652;Hpxn; HX

Source

Human HPN Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Arg45-Leu417.[Accession | P05981]

Molecular Weight

The protein has a predicted MW of 42 kDa. Due to the protein cleaved into the following two chains, so it migrates to 18-22 kDa and 30 kDa based on SDS-PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Activity

ELISA Data: Immobilized Human HPN, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-HPN Antibody, hFc Tag with the EC50 of 10.4ng/ml determined by ELISA.

Formulation

Lyophilized from 0.22μm filtered solution in 20mM NaAc,150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc,150mM NaCl (pH 5.0).