وصف
CD94 is an approximately 25 kDa type 2 transmembrane protein that plays an important role in regulating natural killer (NK) cell activation. Human CD94 consists of a 10 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 148 aa extracellular domain (ECD) with a stem region and one C-type lectin domain. Alternative splicing generates additional isoforms that lack either the stem region, the terminal half of the ECD, or the cytoplasmic and transmembrane regions. CD94 is expressed at varying cell surface density on NK cells during their differentiation and on a subset of CD8+ T cells. It associates into disulfide-linked heterodimers with NKG2A/B, C, or E, and these complexes function as receptors for the nonclassical MHC class I molecule, HLA-E. Ligation of CD94-NKG2A or CD94-NKG2C on NK cells triggers inhibitory or activating signals, respectively.
Specifications
|
Synonyms |
CD94; KLRD1; KP43; NK cell receptor |
|
Uniprot No. |
Q13241.1 |
|
Source |
Recombinant Human CD94 Protein is expressed from HEK293 Cells with His tag and Avi tag at the N-terminal. It contains Ser34-Ile179. |
|
Molecular Weight |
Approximately 19.8 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Tris-Bis PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
|
Reconstitution |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
Storage
Transported with ice packs. Store at -20℃ to -80℃, valid for one year.
After reconstitution, store unopened at -20 to -80°C for 3 to 6 months. After reconstitution, store at 2 to 8°C for 2 to 7 days.。
It is recommended to store in aliquots and freeze for the first use to avoid repeated freezing and thawing.
Note
1. Avoid repeated freezing and thawing.
2. For your safety and health, please wear a lab coat and disposable gloves when operating.
3. This product is for scientific research purposes only.
Product Data
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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