Product Alias

HLA G antigen, HLA G, b2 microglobulin, HLA-G

Species

Cynomolgus

Expression sequence

Gly25-Thr305(HLA-G),Ile21-Met119(B2M) and RIIPRHLQL peptide

Accession

E0WKX9(HLA-G)&Q8SPW0(B2M)&RIIPRHLQL

Label

C-His-Avi

Expression system

HEK293

Molecular weight

The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

preparation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【SPR Data】 Cynomolgus LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus HLA-G Complex Tetramer, His Tag with an affinity constant of 42.50 nM as determined in SPR assay .

Reconstitution method

Centrifuge before opening the lid. Reconstitute the lyophilized protein with distilled water to a concentration of more than 100 μg/mL. It is recommended to aliquot the protein into the amount required for each experiment when using it for the first time to avoid repeated freezing and thawing.