Nature name

Nature Details

Product Alias

HLA0201, MHC I, MAGE-A4, CT1.4, MAGE4A, MAGE4B, MAGE-X2, member 4

Species

Human

Expression sequence

Gly25-Thr305(HLA-A*02:01),Ile21-Met119,(B2M) and GVYDGREHTV peptide

Accession

A0A140T913(HLA-A*02:01)&P61769(B2M)&GVYDGREHTV

Label

C-His-Avi

Expression system

HEK293

Molecular weight

The protein has a predicted MW of 50.5 kDa. Due to glycosylation,the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.

purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

preparation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】Immobilized Human MAGE-A4 (HLA-A*02:01) Complex, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-MAGE-A4 (HLA-A*02:01) Antibody, hFc Tag with the EC50of 12.7ng/ml determined by ELISA.

Reconstitution method

Centrifuge before opening the lid. Reconstitute the lyophilized protein with distilled water to a concentration of more than 100 μg/mL. It is recommended to aliquot the protein into the amount required for each experiment when using it for the first time to avoid repeated freezing and thawing.。