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Mouse Bone Marrow-Derived Macrophages (BMDMs) are a primary macrophage model obtained through in vitro induced differentiation. They are generated by extracting bone marrow cells from the femurs and tibias of mice and culturing them for approximately 7 days in the presence of specific cytokines, allowing hematopoietic progenitor cells in the bone marrow to proliferate and differentiate into mature, functional macrophages. Due to their ease of access, ability to be expanded in large quantities, consistent genetic background (allowing the use of genetically modified mice), and their capacity to mimic key characteristics of in vivo macrophages, BMDMs have become one of the most important and widely used primary cell models in fields such as immunology, inflammatory diseases, infection immunity, and oncology.
This kit is used to differentiate mouse bone marrow cells into macrophages. This kit contains four reagents: recombinant mouse M-CSF, recombinant mouse IL-4, recombinant mouse IL-13, and recombinant mouse IFN-γ. M-CSF is the essential and uniquely critical cytokine that drives bone marrow precursor cells to differentiate, proliferate, and survive along the macrophage lineage; a concentration range of 10 - 50 ng/mL is typically used. This concentration is sufficient to support cell proliferation and differentiation without over-activating the cells. Macrophages are classified into two main types: M1-type macrophages and M2-type macrophages. LPS/IFN-γ can stimulate cells to polarize towards the M1 macrophage phenotype. IL-4 and IL-13 act synergistically to drive cells to polarize towards the M2 phenotype.
Product Information
|
Product Information. |
Catalog Number |
Size |
|
Mouse Macrophage BMDM Cytokine Set |
92643ES10 |
10 μg |
|
92643ES50 |
50 μg |
Components
|
Component Cat No. |
Component Name |
92643ES10 |
92643ES50 |
Quantity |
|
92643ES-A |
Mouse M-CSF Protein |
10 μg |
50 μg(5×10 μg) |
1 |
|
92643ES-B |
Mouse IL-4 Protein |
10 μg(2×5 μg) |
50 μg |
1 |
|
92643ES-C |
Mouse IL-13 Protein |
10 μg |
50 μg |
1 |
|
92643ES-D |
Mouse IFN-γ Protein |
50 μg |
100 μg |
1 |
|
92643ES-E |
Lipopolysaccharide (LPS) |
5 mg |
5 mg |
1 |
Product Includes
|
Component Cat No. |
Product Name |
Source |
Appearance |
Working Concentration (For Reference Only) |
|
91114ES |
Mouse M-CSF Protein |
E. coli |
Lyophilized powder |
20 ng/mL |
|
90144ES |
Mouse IL-4 Protein |
E. coli |
Lyophilized powder |
40 ng/mL |
|
90151ES |
Mouse IL-13 Protein |
E. coli |
Lyophilized powder |
40 ng/mL |
|
91212ES |
Mouse IFN-γ Protein |
E. coli |
Lyophilized powder |
50 ng/mL |
|
60747ES |
Lipopolysaccharide (LPS) |
E. coli |
Lyophilized powder |
100 ng/mL |
The above reagent usage concentrations are for reference only. The concentrations need to be optimized based on specific experimental conditions, purposes, and actual induction effects.
Reconstitution Method
|
Component Name |
Reconstitution Method |
|
Mouse IL-4 Protein Mouse IL-13 Protein Mouse IFN-γ Protein |
Centrifuge the vial prior to opening to pellet contents at the bottom. Reconstitute with sterile water to a concentration of 0.1–1.0 mg/mL, and further dilute according to subsequent experiments. For long-term storage, it is recommended to add carrier protein (0.1% BSA) into the dilution buffer. Aliquot into single-use experimental volumes and store at -80°C; avoid repeated freeze-thaw cycles. |
|
Lipopolysaccharide (LPS) |
Reconstitute with water or cell culture medium to a concentration of 1 mg/mL. |
Notes
1. For your safety and health, please wear a lab coat and disposable gloves during operation.
2. This product is for research use only.
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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