The Enhanced ECL Chemiluminescent Substrate Kit is designed for the detection of horseradish peroxidase (HRP)-labeled primary or secondary antibodies and their associated antigens. The principle involves separating proteins or nucleic acids by electrophoresis, transferring them onto a blotting membrane, and then incubating with a primary antibody followed by an HRP-conjugated secondary antibody, or using an HRP-labeled probe to directly or indirectly bind target nucleic acids on the membrane. After washing, the membrane is incubated at room temperature for several minutes with freshly prepared ECL working solution from this kit. The membrane is then wrapped in plastic wrap, placed face-up into an X-ray film cassette, and exposed to X-ray film in a darkroom for seconds to hours. Following development and fixation, protein or nucleic acid bands are clearly visualized on the X-ray film.

This kit features a unique luminescent substrate system that reduces background signal while incorporating a novel oxidant to significantly enhance stability, allowing the reagent to remain stable for one year at room temperature. In addition to X-ray film detection, the chemiluminescent signal can be directly captured using a fluorescence CCD imager. This product is primarily intended for Western blot (WB) applications and chemiluminescent immunoassay systems.

Features

Superb Sensitivity: Detects antigens at picogram to low femtogram levels for ultra-sensitive Western blot results.

High Signal-to-Noise Ratio: Advanced luminescent substrates deliver strong signals with minimal background noise.

Antibody-Efficient: Optimized formulation enhances antibody binding, reducing antibody consumption.

Excellent Value: Delivers top-tier performance at a competitive price.

Outstanding Stability: Improved oxidant system ensures stable storage at 4°C for up to 12 months.

Specifications

Components

Storage

For frequent use, store at room temperature where it remains stable for up to one year.

For long-term storage, storing at 4 °C is recommended to extend valid life.

[Note]: Solution A (36222-A) must be protected from light.

Application

Chemiluminescence ELISA

Western Blot

Dot Blot-DNA/RNA

Southern blot-DNA

Northern blot-RNA

Documents

Safety Data Sheet

36222_MSDS_HB250923_EN.PDF

Manuals

36222_Manual_Ver.EN20250923.pdf

Rerference

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