Components No.

Name

20543ES10

20543ES60

20543

RNase A  (10 mg/mL)

10 mg

100  mg

Synonyms

Recombinant protein A alkali-tolerant

Source

E.coli

Molecular Weight

Approximately 26.7 kDa, a single non-glycosylated polypeptide chain containing 237amino acids

Amino Acid Sequence

AQGTVDAKFDKEQQNAFYEILHLPNLTEEQRNAFIQSLKDDPSQSANLLAEAKKLND

AQAPKVDAKFDKEQQNAFYEILHLPNLTEEQRNAFIQSLKDDPSQSANLLAEAKKLN  DAQAPKVDAKFDKEQQNAFYEILHLPNLTEEQRNAFIQSLKDDPSQSANLLAEAKKL  NDAQAPKVDAKFDKEQQNAFYEILHLPNLTEEQRNAFIQSLKDDPSQSANLLAEAKK LNDAQAPKC

Concentration for  1  unity  OD at 280 nm

4.4 mg/mL

Physical Appearance

> 97 % by SDS-PAGE and HPLC analyses.

Purity

> 97% by SDS-PAGE and HPLC analyses.

Endotoxin Level

<0.1 EU /1 μg of the protein by the LAL method.

Formulation

A 0.2 µm filtered solution in 20 mM PB,400 mM NaCl, pH7.4 or lyophilized from a 0.2 µm filtered solution in 20 mM PB,400 mM NaCl, pH7.4

Reconstitution

Dissolve in distilled water or saline.