Cat NO.

15217ES84

Specification

2000 U

Recognition Site

5'-GAGTCNNNN↓N-3'
3'-CTCAGNNNNN-5'

Enzyme Activity

10 U/μL

Reaction Conditions

1× Nt.BstNBI Buffer; incubation at 55°C

Inactivation Conditions

Incubation at 80°C for 20 min

Unit Definition

One unit (U) is defined as the amount of enzyme required to completely convert 1 µg of supercoiled pUC19 DNA into open circular form in a 50 μL reaction volume within 1 hour at 55°C.

Components No.

Name

15217ES84(2000 U)

15217-A

Nt.BstNBI (10 U/μL)

200 μL

15217-B

10×Nt.BstNBI Buffer

2×1 mL