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FuniCut™ enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All FuniCut™ enzymes show superior activity in the universal FuniCut™ Buffer and are able to digest DNA in 5~15 minutes.This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. FuniCut™ enzymes have passed multiple strict quality control steps, and can be used to digest plasmid, genomic and viral DNA as well as PCR products.
Specifications
|
Enzyme |
Xbal |
|
Cutting Site |
5'-T↓CTAGA-3' 3'-AGATC↑T-5' |
|
Optimal Reaction Temperature |
37℃ |
|
Sensitive to Heat Inactivation |
YES |
|
Type lls RE |
NO |
|
Methylation Sensitivity |
Not CpG Methylation-Sensitive, Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive,Not EcoKl Methylation-Sensitive, Not EcoBI Methylation-Sensitive |
|
Star Activity |
3 h incubation did not show star activity, and delayed enzyme digestion might show star activity |
|
Isoschizomers |
NO |
Components
|
Components No. |
Name |
15033ES76 |
|
15033-A |
FuniCut™ Xbal |
500 μL |
|
15033-B |
10xFuniCut™ Buffer |
3 x 1 mL |
|
15033-C |
10xFuniCut™ Color Buffer* |
3 x 1 mL |
【Note】: 10× FuniCut™ Color Buffer includes red and yellow tracking dyes, allowing direct loading of reaction products onto agarose gels without additional loading buffer. In 1% agarose gel electrophoresis, the red dye co-migrates with ~2500 bp double-stranded DNA fragments, while the yellow dye migrates similarly to ~10 bp double-stranded DNA fragments.
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.

