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FuniCut™ series endonucleases are fast restriction enzymes developed through genetic engineering that can precisely cut DNA within 5–15 minutes. These enzymes are suitable for rapid digestion of plasmid DNA, PCR products, genomic DNA, and more. All FuniCut™ fast restriction enzymes share the same digestion buffer, which simplifies reaction setup. Additionally, they have a high enzyme activity margin, making them capable of easily handling excess substrate or challenging templates.
Specifications
|
Cat. No. |
15003ES76 / 15003ES20 |
|
Size |
500 T / 20,000 T |
|
Recognition Site |
5'-G↓GATCC-3' 3'-CCTAG↑G-5' |
|
Recommended Reaction Conditions |
1× FuniCut™ Buffer; incubate at 37°C |
|
Enzyme activity |
20 U/μL |
|
Inactivation Conditions |
Not heat-inactivatable. Use phenol-chloroform extraction or column purification. |
|
Cohesive-end ligase |
None |
|
Effect of methylation |
Dam, Dcm, CpG, EcoKI, EcoBI: Not affected |
Components
|
Components No. |
Name |
15003ES76 |
15003ES20 |
|
15003-A |
FuniCut™ BamHI |
500 μL |
20 mL |
|
15003-B |
10×FuniCut™ Buffer |
3×1 mL |
120 mL |
|
15003-C |
10×FuniCut™ Color Buffer* |
3×1 mL |
120 mL |
【Note】: 10× FuniCut™ Color Buffer includes red and yellow tracking dyes, allowing direct loading of reaction products onto agarose gels without additional loading buffer. In 1% agarose gel electrophoresis, the red dye co-migrates with ~2500 bp double-stranded DNA fragments, while the yellow dye migrates similarly to ~10 bp double-stranded DNA fragments.
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