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Hieff UNICON™ Hotstart E-Taq DNA Polymerase features an enhanced hot start Taq DNA polymerase, incorporating dual blocking antibodies. Both the 5'→3' polymerase and 5'→3' exonuclease activities of Taq DNA polymerase are effectively inhibited. A 30-second heating step at the pre-denaturation temperature serves to completely deactivate the antibodies, restoring DNA polymerase and exonuclease activities. This dual blocking capability not only safeguards against nonspecific amplification due to mismatch or primer dimer but also proficiently restrains the reduction in fluorescence signal caused by probe degradation. This ensures enhanced stability of the in vitro detection reagent during transportation or room temperature usage.
Features
High sensitivity
effectively inhibit the non-specific amplification
Applications
Routine PCR amplification of DNA fragments
Hotstart PCR
Specifications
| Polymerase | Taq DNA Polymerase |
| Purity | ≥ 95% (SDS-PAGE) |
| Hot Start | Built-In Hot Start |
| Reaction Speed | Standard |
| Exonuclease Activity | 5' - 3' |
Components
| Components No. | Name | 13824ES72 (250 U) |
13824ES76 (500 U) |
13824ES80 (1000 U) |
13824ES92 (10000 U) |
13824ES93 (25000 U) |
| 13824 | Hotstart E-Taq DNA Polymerase, 20 U/μL | 12.5 μL | 25 μL | 50 μL | 500 μL | 1.25 mL |
Storage
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