dsDNA HS Assay Kit is a fast, sensitive and accurate double-stranded DNA (dsDNA) fluorescence quantitative detection kit. The kits include concentrated assay reagents, dilution buffer, and DNA standards. This kit is highly selective for dsDNA over RNA and is accurate for initial sample concentration from 10 pg/μL to 100 ng/μL. It is an ideal kit for DNA sample quantification such as NGS input DNA quantification and DNA library quantification. The kit has good tolerance to conventional contaminants such as salts, free nucleotides, solvents, detergents, and proteins.

Features

High Sensitivity: Maintains strong linearity across 0.2–100 ng.

Excellent Stability: Consistent performance across production batches.

Contaminant Tolerance: Resistant to interference from common impurities.

Components

Application

dsDNA quantification; DNA concentration determination

Figures

1. Lot-to-lot Consistency: Stable over extended storage; valid for up to 2 years

Figure 1. Good batch-to-batch consistency

Real-time validity period: Batch 1-30 months, Batch 2- 27 months, Batch 3-22 months, all batches demonstrate long-term stability with minimal variation in shelf life.

2. Good linearity: 12640 has good linearity in the range of 0.2-100 ng.

Figure 2. Linear Relationship Test

Linear quantification of 11 dsDNA concentrations (0.5–10 ng/μL) using 12640. Fluorescence was measured with Qubit Fluorometer 3.0. Results show good linearity across 0.2–100 ng total dsDNA.

3. Dye binding specificity

Figure 3. Specificity test

Test Scheme: DNA standard S2 (1–100 ng gradient: 1, 5, 10, 20, 40, 60, 80, 100 ng), along with equal-mass samples of ssDNA, RNA, dsDNA+ssDNA mix, and dsDNA+RNA mix were tested.

Conclusion: Adding equal amounts of ssDNA slightly increases the 12640 reading; error <10%(left). Adding equal amounts of RNA also increases the 12640 reading; error <10%(right).

4. Efficient dye binding and stable fluorescence signal

Figure 4. Comparison of Fluorescence Signal Persistence

Test Scheme: DNA libraries of different concentrations were measured using 12640 on the Qubit 3.0 with a 1 μL sample volume.

Conclusion: Fluorescence from 12640 remains stable for up to 3 h, while Yeasen#12642, Supplier T*#Q32 and Q33* maintain stable signals for up to 1 h.

5. Strong tolerance to common contaminants

6. Ultra-high stability: Thermal Acceleration Stability

Figure 5. Thermal Acceleration Stability Test

Test Scheme: Four dsDNA concentrations were tested using 12640 working solution stored at 42°C, with 4°C storage as the control. Sample volume: 1 μL.

Conclusion: After 2 weeks at 42°C, the measurement error was less than 10% compared to the control, showing that 12640 remains stable under high-temperature conditions.