Hifair™ V Reverse Transcriptase is an updated version of Hieff™M-MLV (H-) Reverse Transcriptase obtained by genetic engineering technology. It has higher cDNA synthesis ability, thermal stability, and reaction temperature limit (up to 60°C) than Hieff™ M-MLV (H-) Reverse Transcriptase. The synthesized cDNA product is up to 10kb. Hifair™ V Reverse Transcriptase enhances the affinity of the templates and is suitable for reverse transcription of RNA templates with complex secondary structures or low-copy genes.

Features 

Outstanding thermal resistance.

Suitable for RT-PCR/RT-qPCR applications.

Glycerol-free, more suitable for lyophilization.

Performance demonstration

1. Capable of withstanding a reaction temperature of 58°C, it is suitable for the reverse transcription of complex RNA templates.

Figure 1. The Reverse Transcriptase of Hifair™  V Reverse Transcriptase and the brand T* were incubated at 55℃ and 58℃ respectively for 30min to detect the residual enzyme activity. The results showed that Hifair™ V Reverse Transcriptase could still maintain 70% activity after incubation at 58℃ for 30min, which was much higher than that of the brand T*.

2. The detection rate of RT-qPCR was better than that of other brands, and the detection range was 1 pg-1 μg.

Figure 2. Using 1 pg-1 μg (7 gradients) total RNA of 293T cells as template, reverse transcription was performed using Hifair™  V one-step RT-gDNA digestion SuperMix for qPCR(Cat#11142), V*, T*. The obtained cDNA was quantitatively analyzed by qPCR. The results showed that the sensitivity of 11142ES could reach 1 pg, which was better than that of other brands.

FAQ

Q: What are the features of this product?

A: M-MLV (H-) Reverse Transcriptase is produced by modifying the wild-type M-MLV reverse transcriptase gene and expressing and purifying it in Escherichia coli. It has no RNaseH activity and possesses the characteristics of high synthesis ability, good thermal stability and long half-life.