Taq DNA Ligase (40 U/µL) _ 11051ES
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Taq DNA Ligase (40 U/µL) _ 11051ES
Taq DNA Ligase (40 U/µL) _ 11051ES
Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between the 5'-phosphate and 3'-hydroxyl groups of two adjacent oligonucleotides hybridized to the same complementary target DNA strand. This catalytic reaction occurs only when the two oligonucleotides are perfectly paired with the complementary target DNA and there is no gap between the two oligonucleotides. Therefore, it can be used to detect single-base substitutions. Taq DNA Ligase uses NAD+ as a cofactor and is active in the temperature range of 45°C to 65°C. Applications Specific detection...

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Taq DNA Ligase (40 U/µL) _ 11051ES

YeasenSKU: 11051ES80
Size: 1000 U
سعر:
$55.00
مخزون:
في الأوراق المالية

Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between the 5'-phosphate and 3'-hydroxyl groups of two adjacent oligonucleotides hybridized to the same complementary target DNA strand. This catalytic reaction occurs only when the two oligonucleotides are perfectly paired with the complementary target DNA and there is no gap between the two oligonucleotides. Therefore, it can be used to detect single-base substitutions. Taq DNA Ligase uses NAD+ as a cofactor and is active in the temperature range of 45°C to 65°C.

Applications

Specific detection of alleles using ligase detection reaction and ligase chain reaction

Mutagenesis by PCR amplification incorporating phosphorylated oligonucleotides

Homologous recombination

Specifications

Concentration

40 U/μL

Source

Recombinant E. coli strain containing the ligase gene cloned from Thermus aquaticus HB8.

Unit Definition

One unit of activity is defined as the amount of enzyme required to ligate 50% of 1 µg of λDNA fragments digested with BstEII (12 bp sticky ends) within 15 minutes at 45°C in a 50 µL reaction volume

Inactivation conditions

Incubate at 45°C for 15 minutes. Add stop dye (50% glycerol, 50 mM EDTA, and bromophenol blue) to terminate the reaction

Components

Components No.

Name

11051ES80

11051ES84

11051ES92

11051-A

Taq DNA Ligase (40 U/µL)

25 μL

50 μL

5×50 μL

11051-B

10×Taq DNA Ligase Buffer（NAD+）

250 μL

500 μL

5×500 μL

Storage

This product should be stored at - 25 ~ -15 ℃ for 1 year.

FAQ

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Applications

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