Hieff Unicon™ Hotstart Direct Taq DNA Polymerase is a hot-start DNA polymerase that tolerates blood and other inhibitors. This product uses antibody blocking and has good amplification sensitivity and specificity. This product can be completely inactivated by heating the blocking antibody at the pre-denaturation temperature for 30 sec, releasing the DNA polymerase activity. The use of this hot-start Taq enzyme can effectively inhibit amplification caused by nonspecific annealing of primers.

Features

Enhanced inhibitor tolerance: The qPCR Mix prepared with D-taq exhibits superior resistance to common PCR inhibitors such as EDTA, sodium citrate, and whole blood.

Nuclease-free: Free from residual exonucleases, endonucleases, and RNase activity.

High sensitivity and specificity: Ideal for PCR applications requiring precise and sensitive detection.

Excellent stability: Maintains consistent performance after storage at 4°C or 37°C for 7 days.

Components

Applications

hot-start PCR and qPCR; RT-qPCR Detection;

Diagnostic pathogen detection (respiratory tract, intestinal tract, etc.); animal pathogen detection (non-swine fever, etc.); food testing (microorganisms, etc.)

Figures

1. Enhanced inhibitor tolerance:

Figure 1. Comparison of Amplification Efficiency with Various Inhibitors

(a) blood-derived inhibitors (EDTA, sodium citrate):

(b) 2% plasma with pseudovirus or standard template (5 μL in 25 μL reaction).

(c) 2% plasma with pseudovirus or standard template (5 μL in 25 μL reaction).

2. Ultra-high sensitivity: down to 3 copies per reaction.

Figure 2. Sensitivity test

Using ASFV primer probe, 20 replicate wells were tested on ASFV single plasmid (3 copies of the final system), and the detection rates of reagents of various brands were compared. The results showed that Yeasen products had a higher detection rate.

3. Stability Test

Figure 3. Real-time stability testing(A) and Freeze-thaw stability(B)

qPCR premix prepared with 10717 DNA polymerase was tested for accelerated stability (37°C for 7 and 14 days) and freeze-thaw stability (-20°C, repeated cycles) using ASFV plasmid as template. Ct and fluorescence values remained consistent, indicating stable performance.