GTP, Guanosine-5'-triphosphate, can be used in various of molecular biology applications, such as in vitro transcription, RNA amplification, siRNA synthesis, etc. In addition, GTP plays an important role in signal transduction as a phosphate or pyrophosphate messenger: GTP can activate G proteins, which can induce multiple protein kinase mediated cascade reactions, causing a variety of cytological behaviors, such as cell proliferation, differentiation, etc. GTP can also be used as a high-energy precursor of single nucleotides to participate in the synthesis of DNase and RNase.
This product is a transparent colorless aqueous solution prepared with GTP tris solution, and free of DNase and RNase contamination.
This product is produced in accordance with GMP process requirements and provided in liquid form.

Feature

Validated, product-specific processes and analytical methods

Product-specific stability

Documentation follows applicable GMP guidelines

AOF production process and raw materials (TSE & BSE)

Nitrosamine statement

Regulatory support documents available

Large-scale production

The increased IVT yield and the decreased dsRNA content under the optimized Tris NTP reaction system

Application

RNA synthesis and amplification

Building block for in vitro transcription

Specification

CAS No	86-01-1 (free acid)
Formula	C10H16N5O14P3 (free acid)
Molecular weight	523.18 g/moL (free acid)
Purity(HPLC)	≥ 99%
Content	200 mM ± 3 mM
Structure

Component

Figures

• Increased the IVT yield

Figure 1. The IVT yield was significantly increased under the optimized Tris NTP reaction system, compared with the sodium NTP reaction system.

• Decreased the dsRNA content

Figure 2. The content of dsRNA was significantly decreased under the optimized Tris NTP reaction system, compared with the sodium NTP reaction system. The content of dsRNA was detected by the Dot Blot method.