وصف
This product is a phage T7 RNA polymerase derived from recombinant expression in E. coli. It is an optimized mutant of wild-type T7, formulated as a mixture of T7 RNA polymerase variants that significantly reduce dsRNA content. Using double-stranded DNA containing the T7 promoter sequence as a template and NTPs as substrates, it synthesizes RNA complementary to the strand downstream of the promoter. Double-stranded linear blunt-ended or 5'-overhanging DNA can serve as substrate templates for T7 RNA polymerase; therefore, linearized plasmids and PCR products can both be used as templates for in vitro RNA synthesis. Modified nucleotides can be added to the substrates during transcription to prepare biotin- or dye-labeled RNA.
Specifications
|
Catalog No. |
10632ES20 / 10632ES60 / 10632ES70 / 10632ES80 |
|
Size |
20 μL / 100 μL / 200 μL / 1 mL |
Component Information
|
Component |
10632ES20 |
10632ES60 |
10632ES70 |
10632ES80 |
|
T7 RNA Polymerase Mix (low dsRNA) |
20 μL |
100 μL |
200 μL |
1 mL |
Storage
Store at -25°C to -15°C. Valid for 1 year.
Documents:
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.