وصف
The NTP Set Solution is a convenient set of 100 mM aqueous solutions of each of ATP, CTP, UTP, GTP, supplied in separate vials. They can be used in a variety of molecular biology applications, such as in vitro transcription, RNA amplification, siRNA synthesis, etc, additionally, used as a reaction substrate or coenzyme for a variety of enzymes.
This product is a clear colorless solution prepared from trisodium salts of ATP, UTP, GTP and CTP with a purity of ≥99%, pH = 7.0±0.1 (25°C), concentration of 100 mM, and is DNase-free and RNase-free.
Feature
- Validated, product-specific process and analytical methods
- Product-specific stability
- Documentation follows applicable GMP guidelines
- AOF production process and raw materials (TSE & BSE)
- Nitrosamine statement
- Regulatory support documents available
- Large-scale production
- Nucleotide in the multiple salt form（Na+, Tris etc) always available to meet different downstream application needs
Application
- RNA synthesis and amplification
- Building block for in vitro transcription
Component
| Components No. | Name | 10133ES03 |
| 10133-A | ATP Solution (100 mM） | 1 mL |
| 10133-B | UTP Solution (100 mM） | 1 mL |
| 10133-C | CTP Solution (100 mM） | 1 mL |
| 10133-D | GTP Solution (100 mM） | 1 mL |
Shipping and Storage
The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for two years.
Figures
- Standard RNA Synthesis
Figure 1. Standard RNA was synthesized in vitro using T7 RNA synthesis kit.
The reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads (Cat#12602). The yield result was analyzed by NanoDrop spectrophotometer as shown in Figure 1.
- Capped RNA Synthesis
Figure 2. Synthesis of capped RNA in vitro.
The reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads (Cat#12602). The yield result was assayed by NanoDrop spectrophotometer as shown in Figure 2A. The integrity result was analyzed by capillary electrophoresis as shown in Figure 2B.
FAQ
Q: The instruction manual says it should be stored at -20°C. Is it okay to store it at -80°C?
A: -80ºC storage has not been tested. It is recommended to store at -20ºC instead.
Q: Can these two products be diluted with sterile water? And how long can they be stored after dilution?
A: Just dilute it with sterile water. How long it can be stored after dilution? I haven't tried it yet. It is recommended to use it up as soon as possible.
Documents:
Payment & Security
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FAQ
The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
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