Yeasen - Leading Innovation in Molecular Enzymes and Reagents

Hieff Trans™ 부스터 DNA/RNA 트랜스펙션 시약 _ 40801ES

Hieff Trans™ 부스터 DNA/RNA 트랜스펙션 시약 _ 40801ES

5.0

부스터 DNA/RNA 트랜스펙션 시약 는 새로운 고분자 재료를 기반으로 개발된 고급 핵산 형질감염 시약입니다. 독특한 분자 설계는 세포 독성을 크게 감소시키고, 전달 과정에서 핵산을 세포 내 효소 분해로부터 보호하는 스마트 항효소 분해 메커니즘을 특징으로 하여 형질감염 효율을 극대화합니다. 이 시약은 DNA, siRNA, miRNA, mRNA, ASO를 포함한 다양한 유형의 핵산에 적합하며, 293T, HeLa, MCF7, HepG2, A549, NIH3T3, RAW267.4, HCT116과 같은 세포주 및 일차 세포에서 효율적인 형질감염이 검증되어 유전자 연구에 신뢰할 수 있는 도구를 제공합니다.

특징

낮은 세포 독성 : 새로운 폴리머 는 기존 리포좀을 대체하여 세포독성 을 줄이고 세포 생존력을 향상시킵니다 .

빠른 방출 및 더 높은 효율성 : 빠른 방출은 세포 내 핵산 분해를 최소화하여 90% 이상의 형질 전환 효율을 달성합니다(유세포 분석법으로 측정).

형질전환이 어려운 세포주를 위한 프리미엄 옵션 : 민감 세포와 일차 세포.

검증된 성능, 광범위한 세포주 적용: 293T, HeLa, MCF7, HepG2, A549, NIH3T3, RAW264.7, HCT116 및 일차 세포를 포함한 200개 이상의 다양한 세포주 에서 일관되고 재현 가능한 결과를 제공하며 높은 효율성을 달성합니다 .

하나의 시약 , 모든 핵산 : 최대 15kb 단편까지도 높은 효율로 DNA, siRNA, miRNA, mRNA 및 ASO를 전달합니다.

구성 요소

구성품 번호	이름	40801ES01	40801ES04
40801-A	부스터 DNA/RNA 트랜스펙션 시약	100 μL	1.5 mL
40801-B	트랜스펙션 인핸서	100 μL	1.5 mL

배송 및 보관

본 제품은 2~8℃에서 12개월간 보관 가능합니다 .

애플리케이션

세포 형질감염; DNA 형질감염; mRNA/SiRNA 형질감염;

수치

1) 고효율 일차세포 BMDM 형질전환

그림 1. Booster DNA&RNA Transfection Reagent를 L *3000 과 비교 하여 골수 유래 대식세포(BMDM)에 플라스미드 DNA를 형질감염시킨 결과입니다 . 이 결과 는 Booster 의 우수한 일차 세포 형질감염 효율을 보여줍니다 .

2) 고효율 DNA 형질 감염

그림 2. DNA transfection Booster DNA&RNA Transfection Reagent를 사용하여 다양한 세포에서 L *3000 대비 DNA transfection 효율을 비교 한 결과, Booster 의 DNA transfection 효율이 더 우수함 을 확인했습니다 .

3) 고효율 mRNA 형질감염

그림 3. mRNA 형질 감염 Booster DNA&RNA Transfection Reagent를 사용하여 다양한 세포에서 L *3000 대비 mRNA transfection 효율을 비교 한 결과, Booster 의 mRNA transfection 효율이 더 우수함 을 확인했습니다 .

4) 고효율 siRNA 형질감염

그림 4. siRNA transfection Booster DNA&RNA Transfection Reagent를 L *3000 과 비교하여 다양한 세포에 적용한 결과, Booster 의 siRNA transfection 효율이 더 우수함을 확인 했습니다 .

고객 피드백

1) 고효율 1차 피부 섬유아세포 형질감염

그림 5. Booster DNA&RNA Transfection Reagent를 사용한 일차 진피 섬유아세포 에서의 플라스미드 EGFR DNA 형질감염 과 L * 3000을 비교한 결과 . 이 결과 는 Booster 의 일차 세포 형질감염 효율이 우수함을 보여줍니다 .

2) 고효율 돼지 폐포 대식세포 형질감염

그림 6. Booster DNA&RNA Transfection Reagent를 사용한 돼지 폐포 대식세포(PAM) 의 1차 세포 DNA 형질감염 과 L * 3000 비교 . 이 결과 는 Booster 의 1차 세포 형질 감염 효율이 더 우수함 을 보여줍니다 .

서류:

안전 데이터 시트

40801_MSDS_HB250715_KO.PDF

매뉴얼

40801_매뉴얼_버전 EN20250807.pdf

무료로 체험해보세요!

Hieff Trans™ 부스터의 무료 샘플을 받아 귀하의 실험실에서 성능을 검증해보세요.
👉 [ 지금 샘플 요청 ]

View Details

Beyond Liposomes: The Next Generation Booster DNA/RNA Transfection

High toxicity, low efficiency, and poor compatibility have long been the three major challenges in cell transfection. Traditional transfey. For primary or sensitive cells, they often require tedious optimization of experimental conditionction reagents, such as lipid-based reagents, offer good efficiency but exhibit high toxicits. PEI-based transfection reagents, while low in toxicity, suffer from poor compatibility; apart from 293 cells, their transfection efficiency in other cell types tends to be weak.
Based on in-depth biological and materials science research, Yeasen has newly developed a next-generation polymeric transfection reagent——Hieff Trans^TM Booster DNA&RNA Transfection Reagent.

Free Samples, Worldwide Shipping

Scan the QR code to submit your information or Click the Link here .
Response within 24 hours.

二维码.png__PID:a9b5f7bf-eedd-4c61-b952-62516bcdf3ed

Key Highlights

Low Cytotoxicity:

The novel polymer replaces traditional liposomes to reduce cytotoxicity and improve cell viability.

Fast Release and Higher Efficiency:

Fast release minimizes intracellular nucleic acid degradation, achieving >90% transfection efficiency (measured by flow cytometry).

Premium Options for Difficult-to-transfect cell lines:

Sensitive and primary cells.

Proven Performance, Broad Cell Line Coverage:

Achieves high efficiency in 80+ various cell lines including 293T, HeLa, MCF7, HepG2, A549, NIH3T3, RAW264.7, and HCT116 and primary cells, with consistent and reproducible results.

One Reagent, All Nucleic Acids:

Delivers DNA, siRNA, miRNA, mRNA, and ASO with high efficiency—even up to 15 kb fragments.

Case Studies and Protocols

Click Cell Name to View Detailed Transfection Protocols

Cell Type	Abbreviation	Nucleic Acid Type
Primary mouse skin fibroblasts	MSFs	DNA
Primary human skin fibroblasts	HSFs	DNA
Primary mouse hepatocytes	PMHs	DNA
Primary mouse neural stem cells	mNSCs	mRNA
Primary human pulmonary artery endothelial cells	HPAECs	DNA
Primary bone marrow–derived macrophages	BMDMs	siRNA
Primary chicken embryo fibroblasts	CEFs	DNA
Primary porcine skeletal muscle satellite cells	PSCs(or PMSCs)	DNA
Primary glioblastoma cells	GBMs	siRNA
Primary porcine alveolar macrophages	PAMs	DNA
Human umbilical vein endothelial cells	HUVEC	DNA
Primary cardiac fibroblasts from neonatal mice	PMCF	siRNA

Cell Type	Abbreviation	Nucleic Acid Type
Human T-lymphoblastic leukemia cells	Jurkat	DNA
Human cervical cancer cells	Hela	DNA
Mouse colon cancer cells	MC38	DNA
Human gastric adenocarcinoma cells	AGS	DNA
Human hepatocellular carcinoma cells	HepG2	DNA
Human colon cancer cells	HCT-116	siRNA
Human gastric cancer cells	HGC-27	siRNA
Human bladder transitional cell carcinoma cells	T24	DNA
Human non-small cell lung carcinoma cells	A549	DNA
Mouse Lewis lung carcinoma cells	LLC	siRNA
Rat adrenal pheochromocytoma cells	PC-12	DNA
Human gastric carcinoma cells	SNU-719	siRNA
Human monocytic leukemia cells	THP-1	siRNA
Human osteosarcoma cells	U2OS	DNA
Human glioblastoma cells	U251	DNA
Human colorectal carcinoma cells	HCT-116	DNA
Human T-Lymphocyte leukemia cells	Jurkat	DNA
Human breast cancer cells	MCF-7	siRNA
Human pancreatic cancer cells	MIA PaCa2	DNA
Human lung squamous carcinoma cells	NCI-H520	siRNA
Human nasopharyngeal carcinoma cells	S18	DNA
Human monocytic leukemia cells	THP-1	DNA
Human glioblastoma cells	U251	siRNA

Cell Type	Abbreviation	Nucleic Acid Type
Mouse monocyte–macrophage cells	RAW264.7	DNA
Mouse monocyte–macrophage cells	RAW264.7	siRNA
Insect ovarian cells	Sf9	DNA
Silkworm (Bombyx mori) ovarian cells	BmN	DNA
Human cardiomyocyte cells	AC16	DNA
Mouse embryonic pre-osteoblast cells	MC3T3-E1	DNA
Mouse embryonic pre-osteoblast cells	MC3T3-E1	siRNA
Human embryonic kidney cells	HEK293	DNA
Human embryonic kidney cells	HEK293	mRNA
Mouse embryonic fibroblast cells	NIH-3T3	DNA
Bovine mammary epithelial cells	MACT	DNA
Human keratinocyte cells	HaCaT	DNA
Chicken embryonic fibroblast cells	DF-1	DNA
Mandarin fish (Siniperca chuatsi) cells	Mandarin fish (Siniperca chuatsi) cells	DNA + mRNA co-transfection
Human cardiomyocytes	AC16	siRNA
Mouse myoblasts	C2C12	DNA
Human umbilical vein endothelial hybrid cells	EAhy926	DNA
Rat cardiomyocytes	H9C2	DNA
Mouse monocyte–macrophages	RAW264.7	DNA
Mouse normal hepatocytes cells	Aml12	siRNA
Mouse bone marrow–derived dendritic cells	DC2.4	DNA
H9C2 rat cardiomyoblasts	H9C2	siRNA
HK-2 human renal proximal tubular epithelial cells	HK-2	siRNA
Neural progenitor cells	NPC	DNA
Mouse monocyte–macrophage cells	RAW264.7	siRNA

Unlock Creativity with Booster's Superior Performance

AI-powered Novel Transfection Reagent Development Platform

Leveraging an AI-powered and high-throughput screening platform for transfection reagent development, Yeasen Biotechnology has successfully launched a series of transfection reagents covering three major fields: basic scientific research, recombinant protein/antibody production, and viral vector manufacturing.

This platform provides researchers with high-quality foundational transfection reagents. It's high-performance products support large-scale antibody and protein production; and it also offers GMP-grade transfection reagents for viral packaging, meeting the demands of large-scale AAV and lentiviral (LV) vector production, thereby robustly supporting drug development and manufacturing applications.

Unique Mechanism Enhances Transfection Efficiency at Every Step.

Hieff Trans^TMBooster is a next-generation polymeric transfection reagent. Its unique mechanism enhances transfection efficiency at every step, delivering higher success rates for your transfection experiments!

Hieff Trans Booster Customer Cases.jpg__PID:72320aa7-e683-4950-8193-4595da362244

1) High Loading Capacity → Dual mechanism: charge interaction + polymer chain entanglement; Higher loading capacity than traditional charge-interaction methods.

2) Efficient Cellular Uptake → Membrane translocation; Faster than conventional endocytosis pathways.

3) Non-Damaging Release → Rapid polymer degradation by intracellular enzymes/reducing GSH; Zero-damage release of nucleic acids.

4) Enhanced Safety → Rapid polymer degradation property; Lower toxicity than traditional lipid-based transfection reagents.

Cell Transfection FAQs

How do I mix multiple plasmids for co-transfection?

Keep total DNA within kit maximum. Each plasmid ≥10 % of total. Adjust molar ratio, reporter plasmid can be reduced 1:5 to 1:10 to avoid "squelching".

When should I assay knock-down after siRNA transfection?

mRNA: 24–48 h.
Protein: 48–72 h.
FAM-siRNA fluorescence visible as cytoplasmic puncta within 6 h.

What’s different about DNA, mRNA, and siRNA transfection?

DNA: Requires nuclear entry for transcription. Timing may be cell cycle-dependent.
mRNA: Only needs to reach the cytoplasm. Faster expression onset, no risk of genomic integration. expression starts 1–4 h, lasts 1–4 days. Ideal for hard-to-transfect cells, short-term expression, promoter-independent studies.
siRNA: Small duplex RNA that mediates sequence-specific mRNA degradation. Expression knockdown can appear within hours. Shorter incubation (4–24 hours), requires specific targeting sequences, used for gene silencing.

How should I improve transfection in primary cells?

Primary cells are often sensitive and have lower uptake efficiency.
Tips:
Use reagents validated for primary cells.
Optimize cell confluency (often 60–80%).
Minimize reagent toxicity (lower doses, shorter exposure).
educe serum concentration during transfection (or use serum - compatible reagents).
Minimize exposure time to the transfection complex (4–6 hours).
Consider electroporation for hard-to-transfect types.

What are the characteristics of common commercial transfection reagents?

Lipid-based:
Lipofectamine^TM, FuGENE^TM, jetPEI^TM. Strengths: High efficiency, broad applicability. Limitations: Can be costly

Polymer-based:
PEI, TurboFect^TM, JetPEI. Strengths: Cost-effective, scalable. Limitations: Higher cytotoxicity

Electroporation:
Lonza Nucleofector^TM, NeonTM. Strengths: Suitable for hard-to-transfect cells. Limitations: Requires specialized equipment

Calcium phosphate:
Classic method. Strengths: Low cost. Limitations: Variable reproducibility

Yeasen Booster is a next-generation polymeric transfection reagent, which has lower toxicity, higher efficiency.