Validated protocols optimized for a wide range of cell culture and in vivo applications.
We provide validated, high-efficiency transfection protocols tailored for a wide range of primary and tissue-derived cells. These optimized solutions are designed to help you achieve ideal transfection results on the first attempt, minimizing the need for time-consuming optimization.
Specifically developed for common tissue-origin cell types, our protocols cover every critical step—from cell culture and expansion to passaging and high-efficiency transfection—with detailed, step-by-step instructions. By balancing high transfection efficiency with low cytotoxicity, these workflows ensure robust performance under physiologically relevant conditions.
All protocols have been rigorously tested and validated by numerous universities and research institutions worldwide, ensuring reliability and practicality for real-world applications. With our Tissue-Derived Cell Transfection Solutions, you can accelerate your research and achieve consistent, publication-quality results—faster and more efficiently.
Optimized Transfection Protocols for Tissue Cell
|
Cell Line |
Species / Cell Type |
Transfection Type |
|
RAW264.7 |
DNA |
|
|
RAW264.7 |
siRNA |
|
|
Sf9 |
DNA |
|
|
BmN |
DNA |
|
|
AC16 |
DNA |
|
|
MC3T3-E1 |
DNA |
|
|
MC3T3-E1 |
siRNA |
|
|
HEK293 |
DNA |
|
|
HEK293 |
mRNA |
|
|
NIH-3T3 |
DNA |
|
|
MACT |
DNA |
|
|
HaCaT |
DNA |
|
|
DF-1 |
DNA |
|
|
Mandarin fish (Siniperca chuatsi) cells |
DNA + mRNA co-transfectionA |
|
|
AC16 |
siRNA |
|
|
C2C12 |
DNA |
|
|
EAhy926 |
DNA |
|
|
H9c2 |
DNA |
|
|
RAW264.7 |
DNA |
|
|
Aml12 |
siRNA |
|
|
DC2.4 |
DNA |
|
|
H9C2 |
siRNA |
|
|
HK-2 |
siRNA |
|
|
NPC |
DNA |
|
|
R264.7 |
siRNA |