This kit is a DNA enzyme digestion library preparation reagent designed for the third-generation Nanopore sequencing platform. This product uses high-quality fragmentation enzymes, gets rid of the cumbersome ultrasonic process, simplifies the operation process, and combines the fragmentation module with the end repair/addition A module, which greatly reduces the time and cost of library preparation. It can be applied to 50 ng-1 μg of conventional animal and plant genomes, microbial genomes and other samples, and realize DNA fragmentation, end repair and A-tail addition reactions in a single tube.

Features

• Easy to operate: One-tube reaction, DNA fragmentation, end repair and A-tail addition reactions can be performed in a single tube
• Wide compatibility: Applicable to conventional animal and plant genomes, microbial genomes, FFPE and other samples
• Easier operation process: The fragmentation module and the end repair/A-tail addition module are combined into one, reducing the time and cost of library construction
• Compatible with automated equipment: The reagents are in Mix form and are compatible with automated library construction equipment

Specifications

Components

 Shipping and Storage

This product should be stored at -25~-15℃ for one year.

Figures

A. DNA fragment test 

Figure 1. DNA fragment detection of different reaction time

Template: 293 gDNA, input DNA 500 ng. According to the recommended fragmentation time and magnetic bead purification ratio, 4-20 Kb fragments can be obtained.

B. higher long fragment read rate

Documents:

Safety Data Sheet

13309_MSDS_HB250714_EN.PDF

Manuals

13309_Manual_Ver.EN20250512.pdf

Citations & References: