Molecular biology
Don’t Know How Much cDNA to Use? Still Measuring cDNA Concentration?
Quantitative PCR (qPCR) is a widely used technique in molecular biology, often perceived as straightforward. However, successful qPCR experiments h...
Common Issues and Troubleshooting in RNA Extraction Using TRIzol
TRIzol reagent mainly contains phenol and guanidine isothiocyanate. These components rapidly lyse cells while inhibiting the activity of RNases rel...
Are You Choosing the Right Method for Nucleic Acid Quantification?
Nucleic acid extraction is a routine procedure in most molecular biology labs. The extracted DNA or RNA is often used in downstream applications su...
Troubleshooting Nucleic Acid Gel Electrophoresis—Solved in One Go!
Introduction
Agarose gel electrophoresis is likely one of the first experiments you performed in the lab. Today, let's take a deep dive into this...
GMP-Grade Recombinant Human bFGF/FGF2 — A Key Factor in Stem Cell Culture
01 Overview
Basic Fibroblast Growth Factor (bFGF), also known as Fibroblast Growth Factor-2 (FGF-2), is a member of the FGF family, which include...
Why Does Your Melt Curve Look So Strange?
Troubleshooting Abnormal Melt Curves in qPCR
In qPCR experiments, melt curves are essential for ensuring product specificity and data accuracy. Abn...
Why Do Amplification Curves Show Spikes?
Perfect amplification curves follow a predictable pattern, but anomalies can appear in all sorts of strange shapes. One common issue, especially wi...
Free Guide: Top 5 PCR Optimization Tips
PCR (Polymerase Chain Reaction) is a cornerstone technique in molecular biology, widely used for gene cloning, diagnostic testing, and research. Wh...
PCR, Nucleic Acid Electrophoresis, Laboratory Techniques Handbook
Chapter 1: PCR Technology
1.1 Principles of PCR
PCR (Polymerase Chain Reaction), or polymerase chain reaction, refers to the DNA polymerase cat...
Hieff Clone Universal II One Step Cloning Kit ——1–7 fragment one-step cloning
Are you still bothered by the low efficiency of homologous recombination of multi-fragment or super-long fragment and the small number of colonies?...
YEASEN Hieff UNICON™ Series Dye-based Fluorescent Quantitative Reagents Aid in Beautiful Curves
Fluorescent quantitative PCR is a highly sensitive experiment that requires a high degree of precision in the operation by laboratory personnel. Th...
Hieff Canace™ Series High-fidelity - Rapid High-fidelity, 5 seconds/kb, ensures fidelity and speed!
When using traditional high-fidelity enzymes for PCR, it's inevitable to encounter some thorny issues. For instance, the fidelity may not be suffic...