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Cell Transfection | Experimental Protocol for Primary Extraction, Culture, and Transfection of Bone Marrow-Derived Macrophages (BMDM)
I. Cell Extraction and Culture
1. Preparation:
1) Mouse Handling: Sacrifice mice by cervical dislocation, then immerse in 75% ethanol for approxi...
Clodronate Liposomes | Lesson 6: C57BL/6 Mice
C57BL/6 (popularly “C57 black 6”, or simply B6) is a black inbred strain established in 1921 by geneticist C. C. Little through continuous brother–...
Clodronate Liposomes | Lesson 5: Brain
Traditionally regarded as an “immune-privileged” organ, the brain is now known to harbor two complementary macrophage systems that jointly maintain...
Clodronate Liposomes | Lesson 4: Intestine
The gastrointestinal tract is responsible for nutrient absorption, a complex task that demands an intact barrier able to let nutrients pass while s...
Deciphering the Hematopoietic Stem Cells Niche: The "Dual Regulatory Role" of Cytokines in Hematopoietic Stem Cells Differentiation
What are Hematopoietic Stem Cells
Hematopoietic Stem Cells (HSCs) are "seed cells" capable of self-renewal and differentiation into all types of ...
Troubleshooting Cell Culture Contamination: A Practical Solution Guide
In molecular biology, even the smallest breakthroughs can dramatically expand our understanding of life. When it
Where Does Contamination Come Fr...
Cytokines: The Code to Unlock the Fate of Neural Stem Cells
What are Neural Stem Cells?
Neural Stem Cells (NSCs) are a type of stem cells present in the nervous system, possessing self-renewal capability a...
From adherent growth to functional regulation: insights into the central role of cytokines in mesenchymal stem cell culture
What are stem cells?
Stem cells are a kind of cells with self-renewal ability and multipotent differentiation potential. Stem cells can maintain ...
From “suspension” to “adhesion”: complete operational guidelines for THP-1 Macrophage induction
Introduction of THP-1 cells
THP-1 cells are a peripheral blood cell line derived from a patient with human acute monocytic leukemia. Since its es...
One-Step Cell Identity Assurance: Species Authentication Made Simple
In the world of biopharmaceutical manufacturing, animal cell cultures serve as the essential “seeds” for life-saving biologics. Yet, one critical t...
New Arrival: ATP Vitality Detection Kit with More Stable Signal
In the vast field of biomedical research and application, cell viability detection has always been a focal point for researchers and clinical worke...
Th17 cell "deforming" guidelines: interesting tips for differentiation experiments that novices can also understand
I. Introduction of Th17 cells
Th17 cells are an important subset of CD4+ helper T cells, named for their predominant secretion of IL-17, regulate...