{"product_id":"20375","title":"Rekombinant CPB, E.Coli ile eksprese edildi. GMP sınıfı _ 20417es","description":"\u003cp class=\"p\"\u003e\u003cspan\u003eKarboksipeptidaz B, peptidil-L-lizin (L-lizin) hidrolaz veya pankreatik karboksipeptidaz B olarak da bilinir, proteinlerdeki temel amino asitlerin C-terminusundaki amino grubunu hidrolize eden özel bir enzim (Lizin (Lys, K), Arginin (Arg, R) ve Histidin (His, H)). 33,8 kD'lik bir moleküler ağırlığa ve 6,0'lık bir izoelektrik noktaya sahiptir ve optimum pH aralığı 7,5 ila 9,0'dır. Karboksipeptidaz B'nin aktivitesi arginin ve lizin tarafından rekabetçi inhibisyona tabidir ve EDTA gibi metal iyon şelatlayıcıları tarafından inhibe edilir.\u003c\/span\u003e\u003c\/p\u003e  \u003cp class=\"p\"\u003e\u003cspan\u003eYeasen Rekombinant CPB, GMP düzenlemeleri altında üretilen, hayvansal kaynaklı bileşenlerden arındırılmış ve hayvansal kaynaklardan viral kontaminasyon riski olmadan E.coli'de ifade edilir. Amino asit dizisi, hayvansal kaynaklı enzimle aynı enzimatik özelliklere sahip olan sıçan pankreas karboksipeptidaz B'ninkiyle aynıdır ve çeşitli biyoteknolojik süreçlerde bir ikame olarak kullanılabilir.\u003c\/span\u003e\u003c\/p\u003e  \u003cp class=\"p\"\u003e  \u003c\/p\u003e  \u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eÖzellikler\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e  \u003cp\u003e\u003cspan\u003e1.Güçlü özgüllük\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003e—\u003c\/span\u003eA proteinlerdeki temel amino asitlerin C-terminusundaki amino grubunu hidrolize eden özel enzim (Lizin (Lys, K), Arginin (Arg, R) ve Histidin (His, H))\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e2. Yüksek saflık—Saflık\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003e≥%95\u003c\/span\u003e.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e3.Hayvan içermez\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003e—\u003c\/span\u003eRekombinant olarak üretilmiştir, ekzojen viral kontaminasyondan arındırılmıştır ve üretim sürecinde hiçbir hayvansal kaynaklı materyal kullanılmamıştır.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e  \u003c\/p\u003e  \u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eUygulamalar\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e  \u003cp\u003e\u003cspan\u003e1. Rekombinant insülin ve analoglarının üretimi.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e2.Proteinlerin C-terminal amino asitlerinin belirlenmesi.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e3.Proteinlerden C-terminal histidin etiketinin çıkarılması.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e4.Diğer rekombinant polipeptit maddelerin üretimi.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e5.Bazı özel bileşiklerin enzimatik sentezi.\u003c\/span\u003e\u003c\/p\u003e  \u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eÖzellikler\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e  \u003cdiv class=\"tableResponsive\"\u003e  \u003ctable class=\"MsoTableGrid\" style=\"width: 100%;\"\u003e  \u003ctbody\u003e  \u003ctr\u003e  \u003ctd valign=\"center\" style=\"width: 26.0971%;\"\u003e  \u003cp\u003e\u003cspan\u003eKaynak\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\" style=\"width: 73.6228%;\"\u003e  \u003cp\u003e\u003cspan\u003eE. coli bakterisi rekombinant ifade\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd valign=\"center\" style=\"width: 26.0971%;\"\u003e  \u003cp\u003e\u003cspan\u003eMoleküler Ağırlık\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\" style=\"width: 73.6228%;\"\u003e  \u003cp\u003e\u003cspan\u003eTeorik değer 33.8±3.4 kDa\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd valign=\"center\" style=\"width: 26.0971%;\"\u003e  \u003cp\u003e\u003cspan\u003eDış görünüş\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\" style=\"width: 73.6228%;\"\u003e  \u003cp\u003e\u003cspan\u003eBeyaz, kirli beyaz veya soluk sarı toz\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd valign=\"center\" style=\"width: 26.0971%;\"\u003e  \u003cp\u003e\u003cspan\u003eEnzim Konsantrasyonu\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\" style=\"width: 73.6228%;\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003e≥ 170 USP birimi\/mg\u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd valign=\"center\" style=\"width: 26.0971%;\"\u003e  \u003cp\u003e\u003cspan\u003eBirim Tanım\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\" style=\"width: 73.6228%;\"\u003e  \u003cp\u003e\u003cspan\u003e25℃, pH 7.6'da, 1 dakikada 1 μmoL hippuril-L-arginin hidrolizini katalize eden enzim miktarı, bir enzim aktivitesi birimi olarak tanımlanır.\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd valign=\"center\" style=\"width: 26.0971%;\"\u003e  \u003cp\u003e\u003cspan\u003eKalite Güvencesi\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\" style=\"width: 73.6228%;\"\u003e  \u003cp\u003e\u003cspan\u003eSDS-PAGE jel tespiti hedef proteinin net tek bir bandını göstermektedir; başka proteaz mevcut değildir, spesifik olmayan bir kesme yoktur.\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003c\/tbody\u003e  \u003c\/table\u003e  \u003c\/div\u003e  \u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eDepolamak\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e  \u003cp\u003e\u003cspan\u003eLiyofilize toz saklanabilir 2 ~ 8\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003e℃ için \u003c\/span\u003eiki Yıllar.\u003c\/span\u003e\u003c\/p\u003e","brand":"Yeasen","offers":[{"title":"1 mg","offer_id":54824562327870,"sku":"20375ES03","price":105.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/5abda97db9c371a240cf9285d74a3035_409959e3-c994-41a8-ab59-466f7096c8c1.jpg?v=1790646695","url":"https:\/\/www.yeasenbio.com\/tr\/products\/20375","provider":"Yeasen - Leading Innovation in Molecular Enzymes and Reagents","version":"1.0","type":"link"}