{"product_id":"20374","title":"Carboxypeptidase B ELISA Kit _ 20374ES","description":"\u003cp\u003eCarboxypeptidase B (also known as peptidyl-L-lysine hydrolase, arginase carboxypeptidase, EC 3.4.17.2) specifically hydrolyzes C-terminal basic amino acids of proteins, such as lysine (Lys, K), arginine (Arg, R), and histidine (His, H). It is widely used in the precise post-processing of biological products such as recombinant insulin and glucagon-like peptide-1 (GLP-1) analogs.\n\u003cbr\u003eThis kit applies the double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) principle for the detection of carboxypeptidase B residue. The carboxypeptidase B standard (20374-B) and test samples are added to the pre-coated anti-carboxypeptidase B antibody plate (20374-A), followed by the addition of diluted biotin-labeled carboxypeptidase B detection antibody (20374-C), and finally Streptavidin-HRP (SA-HRP) (20374-D), forming an antibody + antigen + antibody-Biotin + SA-HRP complex. After plate washing, TMB chromogenic solution (20374-H) is added for color development. TMB is catalyzed by HRP enzyme from colorless to blue and finally converted to yellow under the action of stop solution (20374-I). The intensity of yellow color is positively correlated with the content of carboxypeptidase B detected in the sample. This kit can be used to determine trace residues of recombinant carboxypeptidase B in samples from recombinant protein drug or fusion expression processes using carboxypeptidase B for enzymatic cleavage.\u003c\/p\u003e","brand":"Yeasen","offers":[{"title":"96 T","offer_id":54250647945534,"sku":"20374ES96","price":1400.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.yeasenbio.com\/pt\/products\/20374","provider":"Yeasen - Leading Innovation in Molecular Enzymes and Reagents","version":"1.0","type":"link"}