{"product_id":"16945","title":"Hieff Unicon™ Robust Multiplex qPCR PreMix V1 (UDG Plus) _ 16945ES","description":"\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eThis product utilizes a next-generation dual-antibody blocked hot-start Taq enzyme that simultaneously blocks both the 5'→3' polymerase and 5'→3' exonuclease activities, enabling efficient blocking and precise release of the enzyme. Combined with an exclusively optimized buffer system, ultra-clean manufacturing environment, and stringent quality control standards, the amplification performance and stability of the reagent are further enhanced.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eProduct Information\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoNormalTable\" border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"289\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eCatalog No.\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"563\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e16945ES60\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e\/\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e16945ES80\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e\/\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e16945ES\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e92\/\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e16945ES\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e94\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"289\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSize\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"563\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e100 T \/ 1,000 T \/ 10,000 T \/ 20,000 T\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eComponent Information\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoNormalTable\" border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"164\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eComponent Code\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"179\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eComponent Name\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"149\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003e16945ES60 (100 T)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"164\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003e16945ES80 (1,000 T)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"164\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003e16945ES92 (10,000 T)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"164\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003e16945ES94 (20,000 T)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"164\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e16945\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"179\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e2×TaqMan qPCR Mix\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"149\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1.25 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"164\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e12.5 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"164\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e125 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"164\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e250 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003eFeature\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan\u003eSupports direct amplification from oral swabs: \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003eWhen used with nucleic acid release reagent, direct amplification performance is not affected.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan\u003eExcellent stability of fully premixed formulation: \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003ePremixed primers and probes remain stable after 7 days at 37°C and 20 freeze-thaw cycles.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan\u003eLow background: \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003eNo detection of 11 pathogens and human genomic DNA across 48 replicates.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan\u003eHigh sensitivity: \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003eStable detection at Ct 37–39, slightly superior to comparable market products.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan\u003eSupports fast protocols: \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003eResults available within 30 minutes.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan\u003edUTP\/UDG contamination prevention system: \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003eIncorporates a dUTP\/UDG system that effectively prevents aerosol contamination.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan\u003eMulti-platform and multi-system compatibility: \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003eCompatible with Bio-Rad CFX96, ABI Q5, 7500, SLAN, Tianlong, and other instruments.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003eFigure\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cb\u003e\u003cspan\u003eCase Study 1: Direct Amplification from Swab Samples\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eThroat swab samples were collected and treated with nucleic acid release reagent (Cat#16964) by vortexing. The resulting solution and TE buffer were used separately as template diluents to dilute three mixed plasmid templates to the same concentration. Amplification was performed using both 16945 and comparable market products. Results showed that 16945 exhibited superior tolerance to inhibitors and supports direct amplification from swab samples.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/4_b9e156af-ca96-4309-8b71-36dd98757edb_1024x1024.png?v=1786696957\" alt=\"Figure 1. Comparison of direct amplification performance from swab samples between 16945 and comparable market products.\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\" style=\"text-align: center;\"\u003e\u003cb\u003e\u003cspan\u003eFigure 1. Comparison of direct amplification performance from swab samples between 16945 and comparable market products.\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cb\u003e\u003cspan\u003eCase Study 2: Pathogen Nucleic Acid Detection\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eUsing the 16945 reagent with 11 pathogen assays and GAPDH primers\/probes, water was used as the template, with 48 replicate wells per target. Results showed no non-specific amplification curves, indicating the high cleanliness of the 16945 product.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/5_e6b88800-cb04-46b1-bd4a-4894c60a96d9_1024x1024.png?v=1786696927\" alt=\"\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cb\u003e\u003cspan\u003eCase Study 3: Amplification Efficiency Test\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eUsing the 16945 reagent, linear gradient testing was performed on 3 pathogens at concentrations ranging from 10 to 10⁷ copies\/μL. Results showed amplification efficiencies all above 96% with R²≥0.99, indicating that 16945 supports multiplex amplification with excellent efficiency.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/6_cd172b6c-bf2e-4364-95ae-14228653358e_1024x1024.png?v=1786696949\" alt=\"Figure 2. Linearity test of the 16945 product.\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003eFigure 2. Linearity test of the 16945 product.\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cb\u003e\u003cspan\u003eCase Study 4: Customer Cases\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eTesting was performed on client-provided primers, probes, and samples. Compared to competitor reagents, 16945 showed better stability of the fully premixed formulation at 37°C for 7 days. For 8 African swine fever (ASF) real samples, the detection Ct values were approximately 1 cycle earlier, indicating that 16945 exhibits superior overall performance compared to similar products.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/7_7016debf-3cd8-4376-96a6-8424297d730e_1024x1024.png?v=1786696959\" alt=\"Figure 3. Selected customer case demonstration.\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003eFigure 3. Selected customer case demonstration.\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eStore at -25°C to -15°C, valid for 18 months.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003e\u003cspan id=\"tabCBox1\"\u003eDocuments:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"cwrap\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"list\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"title\"\u003eSafety Data Sheet\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/16945-MSDS-HB260812.pdf?v=1786695991\"\u003e16945_MSDS_HB260812\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"list\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"title\"\u003eManuals\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/16945-Manual-Ver.EN20260811.pdf?v=1786695990\" title=\"download\" rel=\"noopener\" target=\"_blank\"\u003e16945_Manual_HB20260811\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen","offers":[{"title":"100 T","offer_id":54111240782142,"sku":"16945ES60","price":110.0,"currency_code":"USD","in_stock":true},{"title":"1,000 T","offer_id":54111240814910,"sku":"16945ES80","price":835.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/universe_b9e9ebcb-b5e9-497a-b61d-dc198ade0f05.jpg?v=1754453675","url":"https:\/\/www.yeasenbio.com\/pt\/products\/16945","provider":"Yeasen - Leading Innovation in Molecular Enzymes and Reagents","version":"1.0","type":"link"}