{"product_id":"12193","title":"Hieff NGS ™ OnePot Pro DNA Biblioteca Prep Kit V3 (Enzimática) -12194es","description":"\u003cdiv data-v-444b0c7e=\"\" class=\"top_tabc proDetail\"\u003e  \u003cdiv data-v-444b0c7e=\"\"\u003e  \u003cdiv data-v-444b0c7e=\"\"\u003e  \u003cp\u003e\u003cspan\u003eHieff NGS\u003csup\u003eTM\u003c\/sup\u003e O OnePot Pro DNA Library Prep Kit V3 é um kit de preparação de biblioteca baseado em fragmentação enzimática de nova geração \u003c\/span\u003e\u003cspan\u003eespecialmente desenvolvido e projetado para Iluminar Plataforma de sequenciamento \u0026amp;MGI. Comparado aos métodos tradicionais de construção de bibliotecas, este produto emprega enzimas de fragmentação de alta qualidade, eliminando o incômodo processo ultrassônico. Ele simplifica a operação combinando os módulos de fragmentação e reparo final em um. Além disso, as enzimas e o tampão para o módulo de ligação são pré-misturados, reduzindo significativamente o tempo e o custo da construção da biblioteca. Isso o torna mais adequado para a construção automatizada de bibliotecas. Este kit de preparação de biblioteca tem uma excelente taxa de conversão de biblioteca e é aplicável para amostras de todos os animais, plantas, microrganismos comuns, etc., e também amostras FFPE. Com base na geração anterior do kit de construção de biblioteca, este produto apresenta maior eficiência na fragmentação, end repair, dA-tailing e adapter ligation do que as versões anteriores. A enzima de alta fidelidade melhora significativamente a uniformidade e a fidelidade da amplificação.\u003c\/span\u003e\u003c\/p\u003e  \u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eEspecificações\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e  \u003cdiv class=\"tableResponsive\"\u003e  \u003ctable class=\"MsoTableGrid\"\u003e  \u003ctbody\u003e  \u003ctr\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003eCat.No.\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e12194ES08 \/ 12194ES24 \/ 12194ES96\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003eTamanho\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e8 T\/24 E \/ 96 E\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003c\/tbody\u003e  \u003c\/table\u003e  \u003c\/div\u003e  \u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eComponentes\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e  \u003ctable class=\"Table\"\u003e  \u003ctbody\u003e  \u003ctr\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e\u003cb\u003eComponentes No.\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e\u003cb\u003eNome\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e\u003cb\u003e1\u003c\/b\u003e\u003cb\u003e2194\u003c\/b\u003e\u003cb\u003eES08\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e\u003cb\u003e1\u003c\/b\u003e\u003cb\u003e2194\u003c\/b\u003e\u003cb\u003eES24\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e\u003cb\u003e1\u003c\/b\u003e\u003cb\u003e2194\u003c\/b\u003e\u003cb\u003eES96\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e12194-A\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003eEsfregaço\u003csup\u003eTM \u003c\/sup\u003eAmortecedor 3.0\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e80 μL\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e240 μL\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e960 μL\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e12194-B\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003eEsfregaço\u003csup\u003eTM\u003c\/sup\u003e Enzima 3.0\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e80 μL\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e240 μL\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e960 μL\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e12194-C\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003eMistura pronta para ligação \u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e200 μL\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e600 μL\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e3\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003e×\u003c\/span\u003e800 μL\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e12194-D\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e2× Mix de amplificação Ultima HF\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e200 μL\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e600 μL\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e3\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003e×\u003c\/span\u003e800 μL\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003c\/tbody\u003e  \u003c\/table\u003e  \u003cp\u003e\u003cspan\u003e[\u003cb\u003eObservação\u003c\/b\u003e]: Os componentes do kit são compatíveis com ambos Iluminar \u0026amp;MGI plataforma de sequenciamento, se o adaptador completo foi usado, Hieff NGS\u003csup\u003eTM\u003c\/sup\u003e\u003csup\u003e  \u003c\/sup\u003eMistura de primer (\u003ctc\u003eYeasen\u003c\/tc\u003e Cat#12190 ou Cat#12191) se necessário.\u003c\/span\u003e\u003c\/p\u003e  \u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eArmazenar\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e  \u003cp\u003e\u003cspan\u003eEste produto deve ser armazenado a -25~-15℃ por 1 ano.\u003c\/span\u003e\u003c\/p\u003e  \u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eNotas\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e  \u003ch3\u003e\u003cspan\u003e\u003cspan style=\"font-family: 等线;\"\u003e1. \u003c\/span\u003eSobre a operação\u003c\/span\u003e\u003c\/h3\u003e  \u003cp\u003e\u003cspan\u003e1. Por favor, opere com jalecos e luvas descartáveis\u003cspan style=\"font-family: 等线;\"\u003e，\u003c\/span\u003e\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003epara sua segurança.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e2. Descongele os componentes em temperatura ambiente. Após o descongelamento, misture bem no vórtex, gire o tubo brevemente e coloque-o no gelo para uso posterior.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e3. Ao preparar a solução de reação de cada etapa, é recomendado usar uma pipeta para misturar bem ou agitar suavemente. Agitação vigorosa pode causar uma diminuição na saída da biblioteca.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e4. É altamente recomendado usar pontas de pipeta filtradas para evitar contaminação cruzada. Certifique-se de trocar as pontas de pipeta ao processar amostras diferentes.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e5. Operações impróprias podem muito provavelmente causar contaminações por aerossol, impactando a precisão do resultado. O isolamento físico obrigatório das regiões de mistura de reação de PCR e regiões de ensaio de purificação de produto de PCR é recomendado. Equipado com equipamentos como pipetas especializadas para construção de biblioteca. Realize a limpeza de rotina para cada área limpando as superfícies com 0,5% de hipoclorito de sódio ou 10% de alvejante\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e6. Este produto é somente para uso em pesquisa.\u003c\/span\u003e\u003c\/p\u003e  \u003ch3\u003e\u003cspan\u003e\u003cspan style=\"font-family: 等线;\"\u003e2. \u003c\/span\u003eFragmentação de DNA\u003c\/span\u003e\u003c\/h3\u003e  \u003cp\u003e\u003cspan\u003e1. O kit é compatível com 100 pg - 1000 ng de DNA de entrada. É altamente recomendado usar DNA de entrada de alta qualidade com A260\/A280 = 1,8-2,0.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e2. Os experimentos a seguir podem ser impactados se altas concentrações de sais como o agente quelante de metal forem introduzidas com o DNA de entrada. Recomendamos eluir a amostra de DNA em ddH\u003csub\u003e2\u003c\/sub\u003eO para fragmentação.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e3. Consulte a tabela 6 para o tempo de fragmentação de amostras de DNA padrão. O kit tem baixo viés de fragmentação e fornece cobertura GC uniforme para amostras de DNA com uma ampla gama de composições GC. Ajuste o tempo de fragmentação com base nos seus requisitos experimentais.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e4. Para uma fragmentação precisa, prepare a reação no gelo.\u003c\/span\u003e\u003c\/p\u003e  \u003ch3\u003e\u003cspan\u003e\u003cspan style=\"font-family: 等线;\"\u003e3. \u003c\/span\u003eLigação do adaptador\u003c\/span\u003e\u003c\/h3\u003e  \u003cp\u003e\u003cspan\u003e1. Os kits de adaptador longo (adaptador com código de barras) e kits de adaptador curto da Illumina ou MGI estão disponíveis para os clientes escolherem de acordo com suas necessidades experimentais.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e2. Foi recomendado selecionar adaptadores comerciais de alta qualidade. Se forem selecionados adaptadores feitos por você mesmo, confie a uma empresa com experiência em síntese de primers NGS e observe a necessidade de controle rigoroso de contaminação. Além disso, é recomendado preparar a solução de recozimento de DNA em uma bancada limpa e operar apenas um tipo de adaptador por vez para evitar contaminação cruzada.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e3. Descongele os adaptadores no gelo ou a 4 °C; ao operar em temperatura ambiente, a temperatura do laboratório não deve exceder 25 °C para evitar que os adaptadores desnaturem.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e4. A qualidade e a concentração dos adaptadores afetarão diretamente a eficiência da ligação e o rendimento da biblioteca. Concentração muito alta de adaptadores favorece a formação do dímero do adaptador, enquanto adaptador muito pequeno reduz a taxa de ligação e o rendimento da biblioteca. Diluições correspondentes com tampão TE de acordo com a quantidade de DNA de entrada ao usar o adaptador.Tabela 1-2 lista os métodos de diluição recomendados para adaptadores convencionais e UMI para diferentes quantidades de DNA de entrada usando este kit para as plataformas de sequenciamento Illumina ou MGI.\u003c\/span\u003e\u003c\/p\u003e  \u003cp align=\"center\"\u003e\u003cspan\u003eMesa 1 O Illumina recomendado quantidade de adaptador para entrada diferente ADN\u003c\/span\u003e\u003c\/p\u003e  \u003cdiv class=\"tableResponsive\"\u003e  \u003ctable align=\"center\" class=\"Table\"\u003e  \u003ctbody\u003e  \u003ctr\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e\u003cb\u003eEntrada \u003c\/b\u003e\u003cb\u003eADN\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e\u003cb\u003eC\u003c\/b\u003e\u003cb\u003etaxa de diluição do adaptador convencional\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e\u003cb\u003eConcentração\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e\u003cb\u003eProporção de diluição do adaptador UMI\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e\u003cb\u003eConcentração\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e\u003cspan style=\"font-family: 等线;\"\u003e＜\u003c\/span\u003e1 ng\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e7,5 vezes\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e2 \u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003eμM\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e15 vezes\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e1 \u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003eμM\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e1 ng ~ 10 ng\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e3 vezes\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e5 \u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003eμM\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e3 vezes\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e5 μM\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e10 ng ~ 200 ng\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e1,5 vezes\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e10 \u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003eμM\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e2 vezes\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e7,5 μM\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e\u003cspan style=\"font-family: 等线;\"\u003e＞\u003c\/span\u003e200 ng\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e0-Fold\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e15 μM\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e0-Fold\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e15 μM\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003c\/tbody\u003e  \u003c\/table\u003e  \u003c\/div\u003e  \u003cp align=\"center\"\u003e\u003cspan\u003eMesa 2 O recomendado MGI quantidade de adaptador para entrada diferente ADN\u003c\/span\u003e\u003c\/p\u003e  \u003cdiv class=\"tableResponsive\"\u003e  \u003ctable align=\"center\" class=\"Table\"\u003e  \u003ctbody\u003e  \u003ctr\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e\u003cb\u003eEntrada \u003c\/b\u003e\u003cb\u003eADN\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e\u003cb\u003eC\u003c\/b\u003e\u003cb\u003etaxa de diluição do adaptador convencional\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e\u003cb\u003eConcentração\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e\u003cb\u003eProporção de diluição do adaptador UMI\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e\u003cb\u003eConcentração\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e\u003cspan style=\"font-family: 等线;\"\u003e＜\u003c\/span\u003e1 ng\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e5 vezes\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e2 \u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003eμM\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e10 vezes\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e1 \u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003eμM\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e1 ng ~ 10 ng\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e2-Dobrado\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e5 \u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003eμM\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e2-Dobrado\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e5 μM\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e10 ng ~ 200 ng\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e0-Fold\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e10 \u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003eμM\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e1.25 vezes\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e8 \u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003eμM\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e\u003cspan style=\"font-family: 等线;\"\u003e＞\u003c\/span\u003e200 ng\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e0-Fold\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e10 \u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003eμM\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e0-Fold\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"center\"\u003e  \u003cp\u003e\u003cspan\u003e10 \u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003eμM\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003c\/tbody\u003e  \u003c\/table\u003e  \u003c\/div\u003e  \u003ch3\u003e\u003cspan\u003e\u003cspan style=\"font-family: 等线;\"\u003e4. \u003c\/span\u003eLimpeza de DNA baseada em esferas e seleção de tamanho\u003c\/span\u003e\u003c\/h3\u003e  \u003cp\u003e\u003cspan\u003e1. A seleção do tamanho do DNA pode ser realizada antes do reparo final\/cauda dA, após a ligação do adaptador ou após a amplificação.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e2. É recomendável realizar a seleção do tamanho logo após a ligação do adaptador se a quantidade de DNA de entrada for maior que 50 ng; caso contrário, execute a seleção do tamanho após a amplificação.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e3. O Ligation Enhancer contém uma alta concentração de PEG, o que pode causar um impacto significativo na seleção precisa do tamanho. Portanto, se a seleção do tamanho for realizada logo após a ligação do adaptador, é altamente recomendável adicionar uma etapa de limpeza de esferas antes da seleção do tamanho. A etapa de seleção do tamanho pode ser realizada diretamente se for realizada antes do reparo final\/remoção de dA ou após a amplificação da biblioteca.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e4. As esferas magnéticas devem ser equilibradas em temperatura ambiente antes do uso, caso contrário o rendimento diminuirá e o efeito de seleção do tamanho será afetado.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e5. As esferas magnéticas devem ser bem misturadas por vórtice ou pipetagem antes do uso.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e6. Não aspire as esferas ao transferir o sobrenadante, mesmo pequenas quantidades das esferas podem afetar as reações seguintes.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e7. O etanol 80% deve ser preparado recentemente, caso contrário afetará a eficiência da recuperação.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e8. Para uma seleção precisa do tamanho, é recomendado começar com um volume maior que 100 μL. Se for menor, é recomendado aumentar o volume para 100 μL com água ultrapura.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e9. As esferas magnéticas devem ser secas à temperatura ambiente antes da eluição do produto. Secagem insuficiente facilmente fará com que o resíduo de etanol afete as reações subsequentes; secagem excessiva fará com que as esferas magnéticas quebrem e reduzam o rendimento da purificação. Normalmente, a secagem à temperatura ambiente por 3-5 minutos é suficiente para permitir que as esferas sequem completamente.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e10. Se necessário, as amostras de DNA purificadas ou selecionadas por tamanho são eluídas em 0,1× O tampão TE pode ser armazenado a 4°C por 1-2 semanas ou a -20°C por um mês.\u003c\/span\u003e\u003c\/p\u003e  \u003ch3\u003e\u003cspan\u003e\u003cspan style=\"font-family: 等线;\"\u003e5. \u003c\/span\u003eAmplificação de biblioteca\u003c\/span\u003e\u003c\/h3\u003e  \u003cp\u003e\u003cspan\u003e1. Se deve ou não realizar a amplificação da biblioteca depende da quantidade de entrada de DNA, tipos de adaptadores, aplicações de dados de sequenciamento, etc. A etapa de amplificação é necessária se usar adaptadores parciais. Ao usar adaptadores de comprimento total, se o DNA de entrada \u0026lt; 200 ng, é recomendado realizar a amplificação; caso contrário, a amplificação não é necessária.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e2. Os números do ciclo de amplificação devem ser rigorosamente controlados. A amplificação insuficiente pode levar a um baixo rendimento da biblioteca; A superamplificação pode introduzir aumento de viés, erros, leitura duplicada e produtos quiméricos. Tabela 3 lista os números de ciclos recomendados visando o rendimento da biblioteca de 1 μg.\u003c\/span\u003e\u003c\/p\u003e  \u003cp align=\"center\"\u003e\u003cspan\u003eMesa 3 O número recomendado de ciclos para gerar 1.000 ng de rendimento da biblioteca\u003c\/span\u003e\u003c\/p\u003e  \u003cdiv class=\"tableResponsive\"\u003e  \u003ctable align=\"center\" class=\"Table\"\u003e  \u003ctbody\u003e  \u003ctr\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e\u003cb\u003eDNA de entrada\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e\u003cb\u003eNúmero de ciclos necessários para gerar 1 μg de rendimento de biblioteca\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e1000-2000 ng\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e2 - 4\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e500 ng\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e2 - 4\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e250 ng\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e4 - 6\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e100 ng\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e5 - 7\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e50 ng\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e7 - 9\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e10 ng\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e9 - 11\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e5 ng\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e10 - 12\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e1 ng\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e12 - 15\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e100 pg\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd valign=\"top\"\u003e  \u003cp\u003e\u003cspan\u003e16 - 18\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003c\/tbody\u003e  \u003c\/table\u003e  \u003c\/div\u003e  \u003ch3 class=\"H3_backgro\"\u003e  \u003cspan\u003e\u003cstrong\u003eObservação\u003c\/strong\u003e\u003c\/span\u003e  \u003c\/h3\u003e  \u003cp\u003e\u003cspan\u003e1.Tabela 3 mostra o número de parâmetros de loop usando testes de DNA de entrada de alta qualidade de cerca de 200 pb. A qualidade do DNA FFPE varia muito e, quando a qualidade do DNA é ruim ou o comprimento da biblioteca é longo, o número de ciclos precisa ser aumentado adequadamente para obter bibliotecas suficientes.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e2. Se a seleção do tamanho for necessária durante o processo de construção da biblioteca, recomenda-se um número maior de ciclos para a Ampliação da Biblioteca; caso contrário, recomenda-se um número menor de ciclos.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e3. Se forem usados ​​adaptadores incompletos, pelo menos 2 ciclos precisam ser amplificados para formar um adaptador completo.\u003c\/span\u003e\u003c\/p\u003e  \u003ch3\u003e\u003cspan\u003e\u003cspan style=\"font-family: 等线;\"\u003e6. \u003c\/span\u003eAnálise de qualidade da biblioteca\u003c\/span\u003e\u003c\/h3\u003e  \u003cp\u003e\u003cspan\u003e1. A qualidade das bibliotecas construídas é geralmente analisada pela medição das concentrações e distribuições de tamanho.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e2. As concentrações das bibliotecas podem ser medidas por métodos baseados em fluorescência, como Qubit e PicoGreen ou qPCR.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e3. NÃO é recomendado usar métodos de quantificação baseados em absorbância, como o NanoDrop.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e4. É recomendado usar o método qPCR para quantificação de biblioteca: métodos baseados em fluorescência como Qubit e PicoGreen não conseguem diferenciar as estruturas de dsDNA incompletas (inserções sem adaptador ou com apenas uma das extremidades ligadas com adaptador) das bibliotecas completas. O método qPCR amplificará e medirá apenas as bibliotecas completas com ambas as extremidades ligadas com adaptadores (as bibliotecas sequenciáveis), fornecendo assim uma medição mais precisa para carregamento.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e5. A distribuição de tamanho das bibliotecas pode ser analisada usando o Agilent Bioanalyzer ou outros dispositivos baseados nos princípios de eletroforese capilar ou microfluídica.\u003c\/span\u003e\u003c\/p\u003e  \u003ch3\u003e\u003cspan\u003e\u003cspan style=\"font-family: 等线;\"\u003e7. \u003c\/span\u003eOutros materiais\u003c\/span\u003e\u003c\/h3\u003e  \u003cp\u003e\u003cspan\u003e1. Esferas magnéticas de purificação de DNA: Hieff NGS\u003csup\u003eTM\u003c\/sup\u003e Contas de seleção de DNA (\u003ctc\u003eYeasen\u003c\/tc\u003e Cat#12601) ou AMPure\u003csup\u003e®\u003c\/sup\u003e XP Beads (A63880) ou outros produtos equivalentes.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e2.Adaptadores: Adaptador completo para Illumina: \u003ctc\u003eYeasen\u003c\/tc\u003e Cat#13519-13520; 384 Primers CDI duplos: \u003ctc\u003eYeasen\u003c\/tc\u003e Gato#12412~Gato#12413; 384 Primers de Índice Duplo Único (UDI): \u003ctc\u003eYeasen\u003c\/tc\u003e Cat#12312~Cat#12315; Adaptadores UMI UDI: \u003ctc\u003eYeasen\u003c\/tc\u003e Cat#13370~Cat#13371; Adaptador completo para MGI: \u003ctc\u003eYeasen\u003c\/tc\u003e Cat#13360-13362. Mistura de primer de DNA\u003cspan style=\"font-family: 等线;\"\u003e：\u003c\/span\u003eGato#12190 ou Cat#12191.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e3. Análise de qualidade da biblioteca: Agilent 2100 Bioanalyzer DNA 1000 Chip\/High Sensitivity Chip ou outros produtos equivalentes; reagentes quantitativos da biblioteca.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003e4. Outros materiais: etanol absoluto, água ultrapura estéril, pontas de pipeta de baixa retenção, tubo de PCR, suportes magnéticos, termociclador, etc.\u003c\/span\u003e\u003c\/p\u003e  \u003ch3\u003e\u003cspan\u003e\u003cspan style=\"font-family: 等线;\"\u003e8. \u003c\/span\u003eFluxo de trabalho\u003c\/span\u003e\u003c\/h3\u003e  \u003ch3\u003e\u003cimg height=\"445\" src=\"https:\/\/seas.ysbuy.com\/file\/38991\/2888-171998603683335022.png\" width=\"279\" style=\"font-size: 0.875rem; display: block; margin-left: auto; margin-right: auto;\"\u003e\u003c\/h3\u003e  \u003cp align=\"center\"\u003e  \u003c\/p\u003e  \u003cdiv class=\"tableResponsive\"\u003e\u003cbr\u003e\u003c\/div\u003e  \u003cp\u003e\u003cspan\u003eFigura 1. O fluxo de trabalho de \u003c\/span\u003e\u003cspan\u003eUm Pote \u003c\/span\u003e\u003cspan\u003ePró \u003c\/span\u003e\u003cspan\u003eADN\u003c\/span\u003e  \u003cspan\u003eKit de preparação para biblioteca\u003c\/span\u003e\u003c\/p\u003e  \u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cb\u003eFiguras\u003c\/b\u003e\u003c\/span\u003e\u003c\/h3\u003e  \u003cdiv class=\"Section0\"\u003e  \u003cp\u003e\u003cspan\u003eOs tamanhos dos fragmentos de inserção obtidos sob diferentes condições de fragmentação\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan\u003eUsando 500 ng de gDNA padrão como molde, bibliotecas foram construídas com este kit. As condições de fragmentação foram digestão enzimática a 32°C, 35°C e 37°C por 5, 10, 15, 20 e 30 minutos, respectivamente. Os produtos fragmentados foram purificados com esferas magnéticas de 1,2x e eluídos com 21 μL de ddH\u003csub\u003e2\u003c\/sub\u003eO. A concentração foi medida usando Qubit, e a distribuição dos fragmentos de inserção recuperados é mostrada na figura a seguir.\u003c\/span\u003e\u003c\/p\u003e  \u003cdiv class=\"tableResponsive\"\u003e  \u003ctable\u003e  \u003ctbody\u003e  \u003ctr\u003e  \u003ctd\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd\u003e  \u003c\/td\u003e  \u003ctd\u003e\u003cimg height=\"705\" src=\"https:\/\/seas.ysbuy.com\/file\/88493\/8b0f-171998608667666329.png\" width=\"1029\"\u003e\u003c\/td\u003e  \u003c\/tr\u003e  \u003c\/tbody\u003e  \u003c\/table\u003e  \u003c\/div\u003e  \u003cp align=\"center\"\u003e\u003cspan\u003eFigura 2. Perfis de biblioteca a 32°C para diferentes tempos de digestão enzimática\u003c\/span\u003e\u003c\/p\u003e  \u003cp align=\"center\"\u003e  \u003c\/p\u003e  \u003cdiv class=\"tableResponsive\"\u003e  \u003ctable\u003e  \u003ctbody\u003e  \u003ctr\u003e  \u003ctd\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd\u003e  \u003c\/td\u003e  \u003ctd\u003e\u003cimg height=\"809\" src=\"https:\/\/seas.ysbuy.com\/file\/94008\/8aef-171998608668199304.png\" width=\"1046\"\u003e\u003c\/td\u003e  \u003c\/tr\u003e  \u003c\/tbody\u003e  \u003c\/table\u003e  \u003c\/div\u003e  \u003cspan\u003eFigura 3. Perfis de biblioteca a 35 °C para diferentes tempos de digestão enzimática\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003e  \u003c\/span\u003e\u003c\/span\u003e  \u003cp align=\"center\"\u003e  \u003c\/p\u003e  \u003cdiv class=\"tableResponsive\"\u003e  \u003ctable\u003e  \u003ctbody\u003e  \u003ctr\u003e  \u003ctd\u003e  \u003c\/td\u003e  \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd\u003e  \u003c\/td\u003e  \u003ctd\u003e\u003cimg height=\"809\" src=\"https:\/\/seas.ysbuy.com\/file\/90912\/6666-171998608668416170.png\" width=\"1046\"\u003e\u003c\/td\u003e  \u003c\/tr\u003e  \u003c\/tbody\u003e  \u003c\/table\u003e  \u003c\/div\u003e  \u003cspan\u003eFigura 4. Perfis de biblioteca a 37 °C para diferentes tempos de digestão enzimática\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003e  \u003c\/span\u003e\u003c\/span\u003e  \u003c\/div\u003e  \u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e  \u003cp\u003e  \u003c\/p\u003e  \u003c\/div\u003e  \u003c\/div\u003e  \u003c\/div\u003e  \u003cdiv data-v-444b0c7e=\"\" class=\"top_tabc\"\u003e  \u003cspan\u003e\u003c\/span\u003e\u003cbr\u003e  \u003c\/div\u003e  \u003c!----\u003e","brand":"Yeasen","offers":[{"title":"8 t","offer_id":53391587115326,"sku":"12193ES08","price":135.0,"currency_code":"USD","in_stock":true},{"title":"24 t","offer_id":53391587148094,"sku":"12193ES24","price":485.0,"currency_code":"USD","in_stock":true},{"title":"96 t","offer_id":53391587180862,"sku":"12193ES96","price":1865.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/4_27f53046-b886-4b2a-9b4b-fab5ecf79523.png?v=1774344966","url":"https:\/\/www.yeasenbio.com\/pt\/products\/12193","provider":"Yeasen - Leading Innovation in Molecular Enzymes and Reagents","version":"1.0","type":"link"}