{"product_id":"20394","title":"Enterokinase ELISA Kit _ 20394ES","description":"\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eEnterokinase (also known as enteropeptidase, EC 3.4.21.9) is a key enzyme in the intestine that initiates protein digestion. It specifically recognizes the Asp-Asp-Asp-Asp-Lys (DDDDK) sequence and hydrolyzes the peptide bond at the C-terminus of Lys to activate trypsinogen, thereby initiating the zymogen cascade of the digestive system. Its catalytic activity is effective within the pH range of 4.5-9.5 and temperature range of 4-45°C. Recombinant Enterokinase (Cat#20395ES\/20396ES) is a highly purified recombinant bovine enterokinase light chain fragment. Its amino acid sequence is identical to that of the bovine enterokinase light chain, and it possesses the same specific cleavage site as naturally extracted enterokinase. It can be used to remove fusion proteins located at the N-terminus of proteins, thereby eliminating unwanted fusion tags and ensuring the accurate N-terminal sequence of the recombinant fusion protein.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eThis kit uses the double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) principle for the detection of enterokinase residues. Enterokinase standards (20394-B) and test samples are added to a microtiter plate pre-coated with anti-enterokinase antibody (20394-A). Then, diluted biotin-labeled enterokinase detection antibody (20394-C) is added, followed by Streptavidin-HRP (SA-HRP) (20394-D), forming an antibody + antigen + antibody-Biotin + SA-HRP complex. After washing the plate, TMB Substrate Solution (20394-H) is added for color development. TMB is catalyzed by HRP enzyme, changing from colorless to blue, and finally turns yellow under the action of Stop Solution (20394-I). The intensity of the yellow color is positively correlated with the amount of enterokinase detected in the sample. This kit can be used to determine trace residues of recombinant enterokinase in samples from recombinant protein drugs or fusion expression processes where enterokinase cleavage is used.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eFeatures\u003c\/h3\u003e\n\u003cp align=\"justify\"\u003eThe standard curve range is 0.5 ng\/mL to 32 ng\/mL, with intra-assay precision \u0026lt;10% and inter-assay precision \u0026lt;15%.\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" style=\"width: 100.04%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"31.5400%\" valign=\"center\" style=\"width: 36.5795%;\"\u003e\n\u003cp\u003eComponentsNo.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"55.5800%\" valign=\"center\" style=\"width: 43.0655%;\"\u003e\n\u003cp\u003eName\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"12.8400%\" valign=\"center\" style=\"width: 20.3148%;\"\u003e\n\u003cp\u003e20394ES96\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"31.5400%\" valign=\"center\" style=\"width: 36.5795%;\"\u003e\n\u003cp\u003e20394-A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"55.5800%\" valign=\"center\" style=\"width: 43.0655%;\"\u003e\n\u003cp\u003eAnti-Enterokinase coated microtiter strips\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"12.8400%\" valign=\"center\" style=\"width: 20.3148%;\"\u003e\n\u003cp\u003e96 T\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"31.5400%\" valign=\"center\" style=\"width: 36.5795%;\"\u003e\n\u003cp\u003e20394-B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"55.5800%\" valign=\"center\" style=\"width: 43.0655%;\"\u003e\n\u003cp\u003eStandard: Enterokinase\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"12.8400%\" valign=\"center\" style=\"width: 20.3148%;\"\u003e\n\u003cp\u003e2 vials\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"31.5400%\" valign=\"center\" style=\"width: 36.5795%;\"\u003e\n\u003cp\u003e20394-C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"55.5800%\" valign=\"center\" style=\"width: 43.0655%;\"\u003e\n\u003cp\u003eDetection Antibody: Biotin-conjugated Antibodies (50×)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"12.8400%\" valign=\"center\" style=\"width: 20.3148%;\"\u003e\n\u003cp\u003e300 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"31.5400%\" valign=\"center\" style=\"width: 36.5795%;\"\u003e\n\u003cp\u003e20394-D\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"55.5800%\" valign=\"center\" style=\"width: 43.0655%;\"\u003e\n\u003cp\u003eStreptavidin-HRP (100×)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"12.8400%\" valign=\"center\" style=\"width: 20.3148%;\"\u003e\n\u003cp\u003e150 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"31.5400%\" valign=\"center\" style=\"width: 36.5795%;\"\u003e\n\u003cp\u003e20394-E\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"55.5800%\" valign=\"center\" style=\"width: 43.0655%;\"\u003e\n\u003cp\u003eDilution Buffer 1\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"12.8400%\" valign=\"center\" style=\"width: 20.3148%;\"\u003e\n\u003cp\u003e50 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"31.5400%\" valign=\"center\" style=\"width: 36.5795%;\"\u003e\n\u003cp\u003e20394-F\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"55.5800%\" valign=\"center\" style=\"width: 43.0655%;\"\u003e\n\u003cp\u003eWash Buffer Concentrate (20×)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"12.8400%\" valign=\"center\" style=\"width: 20.3148%;\"\u003e\n\u003cp\u003e50 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"31.5400%\" valign=\"center\" style=\"width: 36.5795%;\"\u003e\n\u003cp\u003e20394-G\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"55.5800%\" valign=\"center\" style=\"width: 43.0655%;\"\u003e\n\u003cp\u003eDilution Buffer 2\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"12.8400%\" valign=\"center\" style=\"width: 20.3148%;\"\u003e\n\u003cp\u003e30 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"31.5400%\" valign=\"center\" style=\"width: 36.5795%;\"\u003e\n\u003cp\u003e20394-H\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"55.5800%\" valign=\"center\" style=\"width: 43.0655%;\"\u003e\n\u003cp\u003eTMB Substrate\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"12.8400%\" valign=\"center\" style=\"width: 20.3148%;\"\u003e\n\u003cp\u003e12 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"31.5400%\" valign=\"center\" style=\"width: 36.5795%;\"\u003e\n\u003cp\u003e20394-I\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"55.5800%\" valign=\"center\" style=\"width: 43.0655%;\"\u003e\n\u003cp\u003eStop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"12.8400%\" valign=\"center\" style=\"width: 20.3148%;\"\u003e\n\u003cp\u003e10 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"31.5400%\" valign=\"center\" style=\"width: 36.5795%;\"\u003e\n\u003cp\u003e20394-J\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"55.5800%\" valign=\"center\" style=\"width: 43.0655%;\"\u003e\n\u003cp\u003ePlate Sealer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"12.8400%\" valign=\"center\" style=\"width: 20.3148%;\"\u003e\n\u003cp\u003e5 each\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eShipping and Storage\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eStore at 2~8℃. Valid for 1 year if unopened, and for 6 months after opening. Please use within the valid period.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e*Upon receipt, please check if all components are complete and store them immediately at the corresponding storage temperatures.\u003c\/span\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eFigures\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e\u003cstrong\u003e1.\u003c\/strong\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eGood linearity\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ch3 style=\"text-align: left;\"\u003e\n\u003cimg style=\"margin-bottom: 16px; float: none;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/81cc2027a97c41356260c2af1b634d8c_1024x1024.png?v=1780993496\"\u003e\u003cb\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003eDocuments:\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp\u003eSafety Data Sheet\u003c\/p\u003e\n\u003cp\u003e20394_MSDS_HB260605\u003c\/p\u003e\n\u003cp\u003eManuals\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/20394-Manual-Ver.EN20260521.pdf?v=1780889107\"\u003e20394_Manual_HB20260521\u003c\/a\u003e\u003c\/p\u003e","brand":"Yeasen","offers":[{"title":"96 T","offer_id":53712740712766,"sku":"20394ES96","price":565.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/universe_b9e9ebcb-b5e9-497a-b61d-dc198ade0f05.jpg?v=1754453675","url":"https:\/\/www.yeasenbio.com\/ko\/products\/20394","provider":"Yeasen - Leading Innovation in Molecular Enzymes and Reagents","version":"1.0","type":"link"}