{"product_id":"41218","title":"Hieff™ Exosome Purification Column Kit _ 41218ES","description":"\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eThe Exosome Purification Column Kit is suitable for the isolation and purification of exosome vesicles from clarified, non-complex biological samples (such as cell culture supernatant, clarified milk solution, etc.). The purification column utilizes size exclusion chromatography (SEC) technology to separate particles based on their size as they pass through a column filled with porous polysaccharide resin. When the sample passes through the purification column via gravity flow, larger particles elute first, while smaller particles enter the pores of the resin during their descent, causing delayed elution. The column volume is composed of solid resin (stationary phase) and liquid buffer (mobile phase). Particles do not chemically bind to the resin, ensuring separation stability and reproducibility. This kit is characterized by rapidity, high efficiency, and high purity of the obtained exosomes, making it suitable for downstream applications such as electron microscopy analysis, NTA particle size analysis, nucleic acid analysis, protein analysis, cell-based experiments, and animal experiments.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eSpecial Note: This kit is solely a purification kit, not a concentration kit. Users must purchase an exosome extraction kit or use other methods to perform a concentration pre-treatment on the sample.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eProduct Information\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"0\" cellspacing=\"0\" style=\"width: 99.9672%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"51.2800%\" valign=\"center\" style=\"width: 53.8554%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eCat. No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"48.7000%\" valign=\"center\" style=\"width: 46.0843%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSize\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"51.2800%\" valign=\"center\" style=\"width: 53.8554%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e41218ES05\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"48.7000%\" valign=\"center\" style=\"width: 46.0843%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e5 T\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"27.9600%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eComponent No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"44.4400%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eComponent Name\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"27.5800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e41218ES05\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"27.9600%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e412\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e1\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e8-\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eA\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"44.4400%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003ePurification Column\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"27.5800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e5 T\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"27.9600%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e412\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e1\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e8-\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eB\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"44.4400%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eAdapter\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"27.5800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e5 T\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"27.9600%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e412\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e1\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e8-\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eC\u003c\/span\u003e\u003c\/span\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"44.4400%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eRegeneration Solution\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"27.5800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e300 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"27.9600%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e412\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e1\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e8-\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eD\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"44.4400%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eExosome Purification Column\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"27.5800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e25 T\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eShip at room temperature. Store the kit at room temperature for up to 12 months.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eInstructions\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e1. \u003c\/span\u003e\u003c\/strong\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan\u003eSample Preparation\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eLoad volume per run: 0.5 mL. Equilibrate the sample to room temperature before use.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e1) \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eConcentration: It is recommended to use the Cell Culture Supernatant Exosome Rapid Extraction Kit or the Milk\/Emulsion Exosome Rapid Extraction Kit to concentrate the sample more than 100-fold. Alternatively, use ultracentrifugation, tangential flow filtration, or 100 kD ultrafiltration to concentrate the sample more than 50-fold.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e[Note]: \u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003eUse ultracentrifugation with caution, as it may cause protein aggregation affecting resolution.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e2) \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eRecommended BCA Concentration: Crude exosome BCA concentration from cell culture supernatant containing 10% exosome-depleted serum should be greater than 3 μg\/μL; crude exosome BCA concentration from serum-free cell culture supernatant should be greater than 1 μg\/μL; crude exosome BCA concentration from milk\/emulsion should be greater than 3 μg\/μL.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e[Note]: \u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003eThe primary function of the purification column is to remove small particles such as proteins and free nucleic acids. The purification process will dilute the sample to some extent; therefore, ensure the sample contains a sufficient number of vesicles. For electron microscopy, the particle concentration of the original sample is recommended to be greater than 8.0E+10 particles\/mL.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e\u003cstrong\u003e2.\u003c\/strong\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan\u003eSample Pretreatment\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e1) \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eConcentrate the sample using the recommended method.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e2) \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eFiltration: Use a 0.22 μm filter to remove large particles from the crude exosome preparation.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e[Note]: \u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eThe sample purified by the Exosome Purification Column (EP Column) does not need to be filtered again with a 0.22 \u003c\/span\u003e\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family: Inter;\"\u003em filter.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e3) \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003ePrepare PBS: Use freshly filtered PBS (0.22 μm) to avoid contamination and introduction of large particles. Ensure the PBS temperature is the same as the column temperature (18 - 25°C).\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e[Note]: \u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003eUse freshly prepared 0.22 μm filtered PBS or commercially available filtered PBS to avoid microbial and particulate contamination. If using PBS stored at 4°C, it must be warmed to room temperature before use; otherwise, excessive air bubbles may form in the column, affecting separation.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e3. \u003c\/span\u003e\u003c\/strong\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan\u003eColumn Equilibration and Washing\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e1) \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eEnsure the purification column is within the operating temperature range of 18 - 25°C before the experiment. Do not remove the cap until this temperature is reached.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e2) \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eRemove the black rubber stopper from the top port to balance air pressure, then remove the top cap.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e3) \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eInstall the purification column vertically onto a laboratory stand. Place a waste collection tube (centrifuge tube or beaker) beneath the column for use.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e4) \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eDiscard the storage solution above the frit (remove the bottom cap and let the storage solution drain naturally, or aspirate it).\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e5) \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eAfter the storage solution has drained, wash the upper chamber of the column with PBS 2-3 times. Then, add 20 mL of PBS to wash the resin bed (can be added in batches or connected to the adapter; flow rate approx. 1.5 - 2.5 min\/mL). The liquid will stop flowing once all PBS has entered the column.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e[Note]: \u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003eAdapter usage: Connect the green adapter to a 20 mL syringe barrel..\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e6) \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eAfter the PBS has drained, load the sample immediately, or add 2 mL of PBS, cap the bottom port, and store for later use.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e[Note]:\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eIf using the adapter, once the PBS in the syringe has flowed through, approx. 2-3 mL buffer remains in the column. Remove the adapter and wait for the remaining PBS to drain before loading the sample or adding 2 mL PBS to store.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e\u003cstrong\u003e4.\u003c\/strong\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan\u003eExosome Collection\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e1) \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003ePreparation: Concentrate and filter the sample using the recommended method. Prepare labeled 1.5 mL microcentrifuge tubes.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e2) \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eLoad 0.5 mL Sample: Immediately after the PBS stops flowing, load the equilibrated 0.5 mL sample onto the frit of the purification column (if the sample volume is less than 0.5 mL, adjust to volume with PBS).\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e[Note]: \u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eAvoid stopping the column flow for long periods to ensure optimal vesicle separation.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e3) \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eFlow-through 2.87 mL Void Volume: After all the sample has entered the frit and no liquid is flowing from the bottom, add 2.37 mL of PBS and wait for it to flow through until the flow stops. During this process, a total of 2.87 mL of eluent (0.5 mL sample volume + 2.37 mL buffer) will have flowed through.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e[Note]: \u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003eTo accurately determine the void volume, add a fixed volume of solution and wait for it to flow through completely.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e4) \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eCollect Exosome Fractions: Immediately prepare new microcentrifuge tubes to collect the purified fractions. Add 0.5 mL of PBS to the top of the frit in batches and collect 2-3 fractions (0.5 mL each). The highest purity is found in the first 2 fractions (1 mL total), but it is recommended to merge the first 3 fractions (1.5 mL total). Wait for each volume to flow through until the flow stops.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e5) \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eFilter Exosomes: Transfer the collected exosome fractions to the upper chamber of an Exosome Purification Column (EP Column). Centrifuge at 4°C, 3,000×g for 10 min. The liquid collected at the bottom of the EP column tube is the filtered exosome (Note: The EP column is for single use only).\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e6) \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eMeasure the particle concentration and protein concentration of the collected product. If necessary, use a 100 kD ultrafiltration unit to further concentrate the collected product (optional).\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e\u003cstrong\u003e5.\u003c\/strong\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan\u003eColumn Regeneration and Storage\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e1) \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eColumn Regeneration: After collecting the desired volume, wash the column with 10 mL of Regeneration Solution, followed by 20 mL of PBS. The column is then ready for a second use.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e2) \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eColumn Storage: If storing for future use, wash the column with 10 mL of Regeneration Solution, followed sequentially by 20 mL of PBS, 20 mL of deionized water, and 20 mL of 20% ethanol. After washing, cap the bottom port, pour in the storage solution (20% ethanol), and seal with the top cap.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e[Note]: \u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp align=\"justify\"\u003eThe regeneration solution is alkaline. Do not add deionized water or 20% ethanol directly after washing with regeneration solution to prevent salt precipitation within the resin bed. The deionized water wash removes residual salts, preventing crystallization when ethanol is added.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003eEstimate cleaning times at approx. 1.5 - 2.5 min per mL of liquid flow-through to prevent the resin bed from drying out for extended periods.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eNote\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003es\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e1. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eWhen exosome fractions are intended for downstream high-throughput sequencing or other omics analyses, it is recommended to use a new column to avoid cross-contamination.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e2. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eSalts are inevitably generated during regeneration. Salt precipitation affects resin performance; it is recommended to reuse each column no more than 5 times.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e3. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eHandle the column with care to avoid dropping or impacting it, which could fracture the resin bed.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e4. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eThe regeneration solution is alkaline and corrosive. Handle with extreme care!\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e5. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eFor your safety and health, please wear a lab coat and disposable gloves during operation.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e6. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eThis product is for scientific research use only!\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003e\u003cspan\u003eDocuments:\u003c\/span\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003eSafety Data Sheet\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/41218-MSDS-HB260525.pdf?v=1779759791\"\u003e41218_MSDS_HB260525\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eManuals:\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/41218-Manual-Ver.EN20260518.pdf?v=1779759792\"\u003e41218_Manual_HB20260518\u003c\/a\u003e\u003c\/p\u003e","brand":"Yeasen","offers":[{"title":"5 T","offer_id":53586795594046,"sku":"41218ES05","price":625.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/universe_b9e9ebcb-b5e9-497a-b61d-dc198ade0f05.jpg?v=1754453675","url":"https:\/\/www.yeasenbio.com\/it\/products\/41218","provider":"Yeasen - Leading Innovation in Molecular Enzymes and Reagents","version":"1.0","type":"link"}