{"product_id":"11157","title":"Hifair™ ColorGPS All-in-One RT-gDNA Digestion SuperMix for qPCR _ 11157ES","description":"\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eHifair™\u003c\/span\u003e\u003cspan\u003e colorGPS All-in-one RT-gDNA Digestion SuperMix for qPCR is a newly upgraded, one-tube, trackable reverse transcription reagent specifically designed for qPCR. This product integrates all components required for genomic DNA removal and reverse transcription into a single tube. Only the RNA template and RNase-free water need to be added to perform the reaction. The reagent exhibits excellent tolerance to sample inhibitors, highly efficient genomic DNA removal, and high-yield reverse transcription performance. Additionally, the system contains a red tracking dye that enables visual monitoring during pipetting without affecting reverse transcription or gDNA digestion performance, nor interfering with downstream qPCR experiments, thereby reducing the risk of missed samples.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter;\"\u003eFeatures\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter;\"\u003eEasy Operation: \u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter;\"\u003eOne-tube premix completes gDNA removal and RT in just 15 minutes.\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter;\"\u003eVisual Tracking:\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cspan class=\"15\"\u003e \u003cspan style=\"font-family: Inter;\"\u003eRed dye enables visible pipetting to prevent missed samples.\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter;\"\u003eHigh Efficiency:\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cspan class=\"15\"\u003e \u003cspan style=\"font-family: Inter;\"\u003eDelivers higher RT efficiency and lower Ct values than comparable products.\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"20.5200%\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"42.4800%\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"18.4800%\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e11157\u003c\/span\u003e\u003cspan\u003eES\u003c\/span\u003e\u003cspan\u003e01\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e(10 T)\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"18.4800%\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e11157\u003c\/span\u003e\u003cspan\u003eES\u003c\/span\u003e\u003cspan\u003e60\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e(100 T)\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"20.5200%\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e11157-A\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"42.4800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHifair™\u003c\/span\u003e\u003cspan\u003e ColorGPS All-in-One RT -gDNA Digestion SuperMix for qPCR\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"182\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e60\u003c\/span\u003e\u003cspan\u003e \u003cspan style=\"font-family: Inter;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"182\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e600\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"20.5200%\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e11157-B\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"42.4800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eRNase-free \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eH\u003c\/span\u003e\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e2\u003c\/span\u003e\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eO\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"182\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e200\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"182\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e2X1\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eSpecification\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"174\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eEasy Operation\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"677\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eOne-tube gDNA removal and RT; 15-min single-step protocol.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"174\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eColor Tracking\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"677\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eColorGPS All-in-One RT-gDNA Digestion SuperMix (Red) + qPCR mix (11188-11190ES, Blue) → Purple.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"174\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHigh RT Efficiency\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"677\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eDelivers highly efficient reverse transcription.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"174\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eStrong Digestion\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"677\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u0026gt;99% digestion efficiency for 200 ng gDNA.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"174\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHigh Sensitivity\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"677\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eDetects inputs as low as ≥ 10 pg.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"174\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eStrong Tolerance\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"677\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eTolerates 5% ethanol, 5% isopropanol, and 1.6 mM guanidine thiocyanate.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"174\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eDye Compatibility\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"677\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eTracking dye does not affect RT performance.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"174\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHigh Stability\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"677\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e7 days at 30°C; 4 weeks at 25°C\/4°C; withstands 30 freeze-thaw cycles.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eStorage\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eThis product should be stored at -\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e2\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e5~-\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e1\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e5℃ for \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eone\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e year.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter;\"\u003eFigure\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e\u003cstrong\u003e1.\u003c\/strong\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eWorkflow\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10_b7d86f48-c115-4880-bcb1-fc0911b5effb_1024x1024.png?v=1784712189\" alt=\"Figure 1. Experimental workflow using the Hifair™ ColorGPS All-in-One RT-gDNA Digestion SuperMix\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eFigure 1. Experimental workflow using the Hifair\u003c\/span\u003e\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e™ \u003c\/span\u003e\u003cspan style=\"font-family: Inter;\"\u003eColorGPS All-in-One RT-gDNA Digestion SuperMix\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e\u003cstrong\u003e2.\u003c\/strong\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan\u003eColorGPS™ Visual Tracking: Eliminate Pipetting Errors\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/11_3f2d8739-768e-4c22-8e45-53a963763997_1024x1024.png?v=1784712208\" alt=\"Figure 2. Visual Tracking: Enables clear visualization during pipetting, significantly reducing the risk of missed samples.\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eFigure 2. Visual Tracking: Enables clear visualization during pipetting, significantly reducing the risk of missed samples.\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e11157ES features a red tracking dye for visible pipetting during reverse transcription. The resulting red cDNA turns purple when added to the blue Hieff UNICON™\u003c\/span\u003e\u003cspan\u003e ColorGPS qPCR SYBR Green Master Mix (Cat# 11188ES-11190ES), providing visual tracking throughout the entire RT-qPCR workflow.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e\u003cstrong\u003e3.\u003c\/strong\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan\u003eHigher Reverse Transcription Efficiency\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/5_6d221201-0505-4cec-9a79-1e0b5ad4b3ce_1024x1024.png?v=1784709141\" alt=\"Figure 3. Comparison of Reverse Transcription Efficiency.\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\" style=\"text-align: center;\"\u003e\u003cb\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003eFigure \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e3. Comparison of Reverse Transcription Efficiency.\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e100 ng of RNA from human, Arabidopsis, and mouse samples were used as templates for reverse transcription using Yeasen 11157ES and a leading competitor's product (Supplier N*), following their respective recommended protocols. The resulting cDNA was subjected to qPCR quantification for multiple genes. The results demonstrate that Yeasen 11157ES outperforms Supplier N*, with particularly significant advantages observed when using mouse RNA templates.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e\u003cstrong\u003e4.\u003c\/strong\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan\u003eStrong gDNA Digestion Capacity\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/13_5f7da52e-ebf6-490b-9a9a-4b31e72d4314_1024x1024.png?v=1784712209\" alt=\"Figure 4. gDNA Digestion Efficiency Comparison.\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eFigure 4. \u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003egDNA Digestion Efficiency Comparison\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e. \u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e11157ES exhibits highly efficient gDNA digestion, achieving a digestion rate of \u0026gt;99.8%.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e\u003cstrong\u003e5.\u003c\/strong\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan\u003eHigh Sensitivity\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/14_fd488758-fe1f-4f4b-ac2f-4214b0809e26_1024x1024.png?v=1784712201\" alt=\"Figure 5. High Sensitivity Detection\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eFigure 5. \u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003eHigh Sensitivity Detection\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e11157ES enables highly sensitive detection, capable of reliably detecting RNA inputs as low as 10 pg.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e\u003cstrong\u003e6.\u003c\/strong\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan\u003eStrong Tolerance\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/15_4f4cc1c9-d2e6-4825-9071-73c3563e72ec_1024x1024.png?v=1784712207\" alt=\"Figure 6. Tolerance to Common Residual Substances.\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eFigure 6. \u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003eTolerance to Common Residual Substances\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e.\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e11157ES exhibits strong tolerance to major residual substances commonly found after RNA extraction, including 5% absolute ethanol, 5% isopropanol, and 1.6 mM guanidine salts.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eThis high tolerance ensures robust and reliable reverse transcription performance, even when RNA samples contain trace amounts of extraction reagents, thereby minimizing the need for additional purification steps.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e7. \u003c\/span\u003e\u003c\/strong\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan\u003eExcellent Stability (Freeze-Thaw \u0026amp; Accelerated Stability)\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/16_4ea68d04-3bf4-4c58-ba50-82b9945bb306_1024x1024.png?v=1784712204\" alt=\"Figure 7. Stability assessment of 11157ES under various stress conditions.\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eFigure 7. Stability assessment of 11157ES under various stress conditions.\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e(A). Reverse transcription stability:** Performance was evaluated by measuring ΔCt (Treatment – Control). The product maintained high stability (ΔCt \u0026lt; 0.5) after 30 freeze-thaw cycles and incubation at 37°C for 7 days.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e(B). gDNA removal efficiency:** Under rigorous stress conditions—including 40 freeze-thaw cycles, 7 days at 30°C, and 28 days at 4°C and 25°C—the gDNA removal efficiency of 11157ES consistently remained \u0026gt;99% for 200 ng of input gDNA.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\" align=\"justify\"\u003e\n\u003cb\u003e\u003cspan\u003eFAQ\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eQ1: Can this product be used with qPCR Master Mixes from other manufacturers for downstream qPCR experiments?\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eA1: Theoretically, yes. However, performance with third-party reagents has not been validated. If you intend to use other brands, we recommend conducting a preliminary test to evaluate performance.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eQ2: Can the cDNA product be used for standard PCR experiments?\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eA2: No. 11157ES is a reverse transcription reagent specifically designed for qPCR and is not recommended for standard PCR amplification. If you require standard PCR, we recommend using the Hifair™\u003c\/span\u003e\u003cspan\u003e Advance Fast 1st Strand cDNA Synthesis Kit (Cat# 11149ES, Cat# 11150ES) for efficient reverse transcription.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eQ3: Is the reaction program strictly required to be 37°C for 10 min and 60°C for 5 min?\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eA3: The recommended conditions of 37°C for 10 min and 60°C for 5 min represent the optimal parameters for gDNA removal and reverse transcription, ensuring maximum RT efficiency. If necessary, you may try an alternative protocol of 50°C for 5–15 min to determine whether the reverse transcription efficiency meets your experimental requirements.\u003c\/span\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ch3 class=\"p\"\u003e\n\u003cb\u003e\u003cspan\u003eDocuments:\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSafety Data Sheet\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/11157-MSDS-HB260722.pdf?v=1784712694\"\u003e11157_MSDS_HB260722\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eManuals\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/11157-Manual-Ver.EN20260722.pdf?v=1784712682\"\u003e11157_Manual_HB20260722\u003c\/a\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eRelated Blogs:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeasenbio.com\/blogs\/molecular-biology\/visible-reverse-transcription-simplified-meet-the-new-hifair%E2%84%A2-colorgps-one-tube-rt-kit\"\u003eVisible Reverse Transcription, Simplified: Meet the New Hifair™ ColorGPS One-Tube RT Kit\u003c\/a\u003e\u003c\/p\u003e","brand":"Yeasen","offers":[{"title":"10 T","offer_id":53946237878590,"sku":"11157ES01","price":65.0,"currency_code":"USD","in_stock":true},{"title":"100 T","offer_id":53946237911358,"sku":"11157ES60","price":305.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/65d29ecb9a6148d1b93fe4a0f9201f8f.png?v=1784767321","url":"https:\/\/www.yeasenbio.com\/fr\/products\/11157","provider":"Yeasen - Leading Innovation in Molecular Enzymes and Reagents","version":"1.0","type":"link"}