{"product_id":"92140","title":"Recombinant Human NRG1-beta1 Protein _ 92140","description":"\u003cdiv class=\"Section0\"\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003eThe neuregulin family of structurally related glycoproteins comprises products from four distinct but related genes, Nrg-1, Nrg-2, Nrg-3, and Nrg-4. Through alternative splicing or the use of alternative promoters, Nrg-1 has been shown to encode more than 14 soluble or transmembrane proteins. The extracellular domain of the transmembrane NRG1 isoforms can be proteolytically cleaved to release soluble growth factors. All NRG1 isoforms contain an EGF-like domain ( alpha - or beta -splice variant that differ in their C-terminal region) that is required for their direct binding to the ErbB3 or ErbB4 receptor tyrosine kinases. The ErbB3 or ErbB4 subsequently recruits and heterodimerizes with ErbB2, resulting in tyrosine phosphorylation and NRG1 signaling. NRG1 isoforms can be classified into three major subtypes. Type I (Neu Differentiation Factor, NDF; Heregulin, HRG; Acetylcholine Receptor Inducing Activity, ARIA) and type II (Glial Growth Factor, GGF) NRG1s have an immunoglobulin (Ig)-like domain N-terminal to the EGF-like domain. Type I NRG1s differ from type II NRG1s by having a glycosylation-rich domain between the Ig-like and the EGF-like domains. Type III NRG1s (Sensory and Motor neuron-Derived Factor) lacks the Ig-like domain but has a cysteine rich domain (CRD) instead. NRG1 isoforms show distinct spatial and temporal expression patterns. These proteins play important roles during development of both the nervous system and the heart. They have been shown to regulate the selective expression of neurotransmitter receptors in neurons and at the neuromuscular junction, and promote the differentiation and development of Schwann cells from neural crest stem cells. NRG1s have also been shown to be involved in the establishment of the oligodendroglial lineage.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 align=\"justify\"\u003e\u003cspan\u003e\u003cb\u003eProduct \u003c\/b\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable width=\"100%\" class=\"Table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22.3074%;\" valign=\"center\"\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003e\u003cb\u003eSynonyms\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 77.4705%;\" valign=\"center\"\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003eARIA; GGF; GGF2; HGL; HRG; Neuregulin-1 beta 1; NRG1 beta 1\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22.3074%;\" valign=\"center\"\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003e\u003cb\u003eAccession\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 77.4705%;\" valign=\"center\"\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003eQ02297\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22.3074%;\" valign=\"center\"\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003e\u003cb\u003eGeneID\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 77.4705%;\" valign=\"center\"\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003e3084\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22.3074%;\" valign=\"center\"\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003e\u003cb\u003eSource\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 77.4705%;\" valign=\"center\"\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003eE.coli-derived Human NRG1-β1, Ser177-Glu241.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22.3074%;\" valign=\"center\"\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003e\u003cb\u003eMolecular Weight\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 77.4705%;\" valign=\"center\"\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003eApproximately 7.5 kDa.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22.3074%;\" valign=\"center\"\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003e\u003cb\u003eAA Sequence\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 77.4705%;\" valign=\"center\"\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003eSHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYKHLGIE FMEAE\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22.3074%;\" valign=\"center\"\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003e\u003cb\u003eTag\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 77.4705%;\" valign=\"center\"\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003eNone\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22.3074%;\" valign=\"center\"\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003e\u003cb\u003ePhysical Appearance\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 77.4705%;\" valign=\"center\"\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003eSterile Filtered White lyophilized (freeze-dried) powder.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22.3074%;\" valign=\"center\"\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003e\u003cb\u003ePurity\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 77.4705%;\" valign=\"center\"\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003e\u0026gt; 97 % by SDS-PAGE and HPLC analyses.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22.3074%;\" valign=\"center\"\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003e\u003cb\u003eBiological Activity\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 77.4705%;\" valign=\"center\"\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003eThe ED\u003csub\u003e50\u003c\/sub\u003e as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 0.5 ng\/mL, corresponding to a specific activity of \u0026gt; 2.0 × 10\u003csup\u003e6\u003c\/sup\u003e\u003csup\u003e \u003c\/sup\u003eIU\/mg. Fully biologically active when compared to standard. \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22.3074%;\" valign=\"center\"\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 77.4705%;\" valign=\"center\"\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003e\u0026lt; 0.1 EU per 1μg of the protein by the LAL method.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22.3074%;\" valign=\"center\"\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003e\u003cb\u003eFormulation\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 77.4705%;\" valign=\"center\"\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003eLyophilized from a 0.2 μm filtered solution in 1 × PBS, pH 7.4, with 5 % trehalose.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable width=\"100%\" class=\"Table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003e\u003cb\u003eReconstitution\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003eWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg\/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20\u003cspan face=\"Times New Roman, Times, serif\" style=\"font-family: 'Times New Roman', Times, serif;\"\u003e℃\u003c\/span\u003e. Further dilutions should be made in appropriate buffered solutions.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 align=\"justify\"\u003e\u003cspan\u003e\u003cb\u003eS\u003c\/b\u003e\u003cb\u003ehipping\u003c\/b\u003e\u003cb\u003e and S\u003c\/b\u003e\u003cb\u003etorage\u003c\/b\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003eThe products are shipped with ice pack and can be stored at -20\u003cspan face=\"Times New Roman, Times, serif\" style=\"font-family: 'Times New Roman', Times, serif;\"\u003e℃\u003c\/span\u003e for 1 year.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003e1 month, 2 to 8 °C under sterile conditions after reconstitution.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003e3 months, -20 to -70 °C under sterile conditions after reconstitution.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003eRecommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 align=\"justify\"\u003e\u003cspan\u003e\u003cb\u003eCaution\u003c\/b\u003e\u003cb\u003es\u003c\/b\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003e\u003cspan face=\"Times New Roman, Times, serif\" style=\"font-family: 'Times New Roman', Times, serif;\"\u003e1.\u003c\/span\u003eAvoid repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003e\u003cspan face=\"Times New Roman, Times, serif\" style=\"font-family: 'Times New Roman', Times, serif;\"\u003e2.\u003c\/span\u003eFor your safety and health, please wear lab coats and disposable gloves for operation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e\u003cspan\u003e\u003cspan face=\"Times New Roman, Times, serif\" style=\"font-family: 'Times New Roman', Times, serif;\"\u003e3.\u003c\/span\u003eFor research use only!\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 align=\"justify\"\u003e\u003cstrong\u003eDocuments\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Yeasen","offers":[{"title":"10 ug","offer_id":53302321414462,"sku":"92140ES10","price":75.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53302321447230,"sku":"92140ES60","price":390.0,"currency_code":"USD","in_stock":true},{"title":"500 ug","offer_id":53302321479998,"sku":"92140ES76","price":940.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/92140_06e8840e-75da-4f92-a9e4-110789677321.png?v=1776042824","url":"https:\/\/www.yeasenbio.com\/fa\/products\/92140","provider":"Yeasen - Leading Innovation in Molecular Enzymes and Reagents","version":"1.0","type":"link"}