{"product_id":"41211","title":"Lysis buffer for Exosome _ 41211ES","description":"\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eExosome is a small vesicle with a diameter of about 30-150 nm secreted by living cells, which has a typical lipid bilayer structure and exists in cell culture supernatant, serum, plasma, saliva, urine, amniotic fluid and other biological fluids. Exosome carries a variety of important information such as protein, lipids, DNA and RNA, which not only plays an important role in the material and information transmission between cells, but also is expected to become an early diagnostic markers for a variety of diseases.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eThe lysis buffer is developed for exosome samples, which can efficiently lyse exosome proteins. The lysis buffer contains protease inhibitor, which can effectively inhibit protein degradation, and is suitable for BCA detection, Western Blot and other experiments after sample lysis. This reagent contains a high concentration of detergent, which is not suitable for Bradford method to determine protein concentration.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eSpecifications\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"0\" cellspacing=\"0\" style=\"width: 99.9672%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"311\" valign=\"top\" style=\"width: 56.0241%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eCat.N\u003c\/span\u003e\u003cspan\u003eo\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"651\" valign=\"top\" style=\"width: 43.9157%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e211\u003c\/span\u003e\u003cspan\u003eES20\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"311\" valign=\"top\" style=\"width: 56.0241%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSize\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"651\" valign=\"top\" style=\"width: 43.9157%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"0\" cellspacing=\"0\" style=\"width: 99.9672%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"25.7000%\" valign=\"top\" style=\"width: 35.4217%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"50.0800%\" valign=\"top\" style=\"width: 31.2651%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"24.2000%\" valign=\"top\" style=\"width: 33.253%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e211\u003c\/span\u003e\u003cspan\u003eES\u003c\/span\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"25.7000%\" valign=\"center\" style=\"width: 35.4217%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e41211\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"50.0800%\" valign=\"center\" style=\"width: 31.2651%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eLysis buffer for exosome\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"24.2000%\" valign=\"center\" style=\"width: 33.253%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003e m\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eTransportation in ice bag, storage at -25~-15℃, valid for one year.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eNotes\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e1. This product contains protease inhibitor, so please store it separately to avoid repeated freezing and thawing.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e2. Lysis samples need to be performed on ice or at 4°C.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e3. The protein concentration after lysis should be less than 2ug\/uL to ensure sufficient lysis.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e4.\u003c\/span\u003e\u003cspan\u003e Please operate with lab coats and disposable gloves\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e，\u003c\/span\u003e\u003cspan style=\"font-family: Inter;\"\u003efor your safety.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e5. \u003c\/span\u003e\u003cspan\u003eThis product is for research use only.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\" align=\"justify\"\u003e\n\u003cb\u003e\u003cspan\u003eInstructions\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e1. Before the experiment, please melt the lys\u003c\/span\u003e\u003cspan\u003eis\u003c\/span\u003e\u003cspan\u003e at 2 ~ 8℃ and put it on ice for later use.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e2. Add the lysis buffer to the exosome sample at a volume ratio of 1:1, gently blow and mix, and place on ice for lysis for 10 minutes.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e. \u003c\/span\u003e\u003cspan\u003eCentrifuge at 4℃, 12000×g for 5 minutes, take the supernatant, and determine the protein concentration by BCA method or perform subsequent experiments such as Western Blot.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003e\u003cspan\u003eDocuments:\u003c\/span\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003eSafety Data Sheet\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/41211-MSDS-HB260525.pdf?v=1779759791\"\u003e41211_MSDS_HB260525\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eManuals:\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/41211ES-Manual-Ver.EN20250525.pdf?v=1779759792\"\u003e41211_Manual_HB20260525\u003c\/a\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e","brand":"Yeasen","offers":[{"title":"20 mL","offer_id":53585544610110,"sku":"41211ES20","price":75.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/universe_b9e9ebcb-b5e9-497a-b61d-dc198ade0f05.jpg?v=1754453675","url":"https:\/\/www.yeasenbio.com\/fa\/products\/41211","provider":"Yeasen - Leading Innovation in Molecular Enzymes and Reagents","version":"1.0","type":"link"}