{"product_id":"41205","title":"Hieff™ Quick Exosome Isolation Kit Plus(for Cell Culture Media) _ 41205ES","description":"\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan\u003eExosomes are small vesicles (30-150 nm in diameter) secreted by living cells, characterized by a typical lipid bilayer structure. They exist in cell culture supernatants, serum, plasma, saliva, urine, amniotic fluid, and other biological fluids. Exosomes carry various important biomolecules such as proteins, lipids, DNA, and RNA. They play a crucial role in intercellular material and information transfer and hold promise as early diagnostic biomarkers for various diseases.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan\u003eThis kit employs a unique isolation technology to rapidly obtain a large quantity of intact exosome particles from cell culture supernatants. It is suitable for downstream applications including cell co-culture, electron microscopy analysis, Western Blot, quantitative PCR (qPCR), and high-throughput sequencing. The maximum processing volume is 200 mL of cell culture supernatant.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eProduct Information\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable cellspacing=\"0\" border=\"0\" class=\"MsoTableGrid\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"55.5800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eProduct Information\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"21.7600%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eCatalog Number\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"22.6200%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSize\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" valign=\"center\" width=\"55.5800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHieff™\u003c\/span\u003e\u003cspan\u003e Quick Exosome Isolation Kit Plus(for Cell Culture Media)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"21.7600%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e41205ES02\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"22.6200%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e2 T\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"21.7600%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e41205ES10\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"22.6200%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e10 T\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"21.7600%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e41205ES20\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"22.6200%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e20 T\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable cellspacing=\"0\" border=\"0\" class=\"MsoTableGrid\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"17.7800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eComponent No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"35.4800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eComponent Name\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"15.5600%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e41205ES02\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"15.5600%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e41205ES10\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"15.5600%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e41205ES20\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"17.7800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e41205-A\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"35.4800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eExosome Concentration Solution*\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"15.5600%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"15.5600%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e25 mL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"15.5600%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e50 mL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"17.7800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e41205-B\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"35.4800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eExosome Purification Filter*\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"15.5600%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e2 Tubes\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"15.5600%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e10 Tubes\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"15.5600%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e20 Tubes\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e[Note]:\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e* Nuclease-free, Sterile\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan\u003eTransport at room temperature. Store at room temperature.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eShelf life:\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e2 years.\u003c\/span\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eInstructions\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ch4 align=\"justify\" class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e\u003cstrong\u003eI.\u003c\/strong\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eSample Pre-processing\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h4\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e1. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eSampling: Collect fresh samples and place them on ice for use. For frozen samples, thaw them in a 25°C water bath, then place them on ice after complete melting.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e2. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eInitial Sample Volume: The recommended volume for a single extraction is no less than 20 mL of cell culture supernatant.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e3. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eRemove Cell Debris: Transfer the sample to a centrifuge tube and centrifuge at 4°C, 3,000×g for 10 min to remove cell debris. (If there is a large amount of precipitate, repeat centrifugation at 3,000×g for 10 min until no obvious precipitate remains, collecting the supernatant each time).\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e4. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eRemove Impurity Debris: Transfer the supernatant to a new centrifuge tube and centrifuge at 4°C, 10,000×g for 10 min to remove impurity debris.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e5. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eSupernatant Transfer: Transfer the supernatant (free of impurity debris) to a new centrifuge tube.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4 align=\"justify\" class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e\u003cspan style=\"mso-list: Ignore;\"\u003eII. \u003c\/span\u003e\u003c\/strong\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan\u003eExosome Precipitation\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h4\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e1. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eSupernatant Pre-treatment: Add 41205-A Reagent to the clarified supernatant. The specific volume is as follows (adjust proportionally for other sample volumes):\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable cellspacing=\"0\" border=\"0\" class=\"MsoTableGrid\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"33.5600%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSample Name\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"32.5000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSample Volume\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"33.9000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e41205-A Reagent Volume\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"33.5600%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eCell Culture Supernatant\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"32.5000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e20 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"33.9000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e5 \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e\u003cbr\u003e2. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eMixing: After adding the reagent, tighten the cap and mix thoroughly using a vortex mixer for 1 min. Incubate at 4°C for at least 8 hours (Note: Extending the incubation time can improve exosome yield, but do not exceed 24 hours).\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e3. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003ePrecipitate Exosomes: Centrifuge the mixture at 4°C, 10,000×g for 60 min. Discard the supernatant (Note: Aspirate as much supernatant as possible).\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e4. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eSecondary Centrifugation: Centrifuge the tube containing the pellet again at 4°C, 10,000×g for 2 min. Discard the supernatant (Note: Aspirate as much supernatant as possible).\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e5. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eResuspend Pellet: Resuspend the pellet by pipetting up and down with an appropriate volume of 1×PBS. Transfer the resuspended solution to a new 1.5 mL centrifuge tube (Recommended: ~200 μL of 1×PBS for every 20 mL of cell culture supernatant).\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e6. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eHarvest Exosome Particles: Centrifuge the 1.5 mL tube at 4°C, 12,000×g for 2 min. Retain the supernatant, which is enriched with exosome particles (If there is significant precipitate, repeat centrifugation at 12,000×g for 2 min until no obvious precipitate remains, collecting the supernatant each time).\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4 align=\"justify\" class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e\u003cspan style=\"mso-list: Ignore;\"\u003eIII. \u003c\/span\u003e\u003c\/strong\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan\u003eExosome Purification\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h4\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e1. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003ePurify Exosomes: Transfer the crude exosome particles (supernatant from step 2.6) to the upper chamber of the 41205-B (EPF Column). Centrifuge at 4°C, 3,000×g for 10 min. Collect the liquid at the bottom of the collection tube; this liquid contains purified exosome particles (Note: The EPF Column is for single use only).\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e2. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eStorage: Aliquot the purified exosomes into suitable volumes and store at -80°C for subsequent experiments.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eNote\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003es\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e1. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eTo ensure the exosomes are derived from your cells, it is best to culture cells using exosome-depleted serum.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e2. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003eFor further purification,\u003cspan\u003e affinity purification using antibody-coated magnetic beads is recommended.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e3. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003eThis product is specifically \u003cspan\u003edesigned for exosome isolation from cell culture supernatants. It is not suitable for exosome extraction from serum or plasma. For serum\/plasma exosome isolation, please select the Serum\/Plasma Exosome Rapid Extraction Kit.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e4. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eFor your safety and health, please wear a lab coat and disposable gloves when handling this product.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e5. \u003c!--[endif]--\u003eFor research use only!\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003e\u003cspan\u003eDocuments:\u003c\/span\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003eSafety Data Sheet\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/41205-MSDS-HB260525.pdf?v=1779759791\"\u003e41205_MSDS_HB260525\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eManuals:\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/41205-Manual-Ver.EN20260515.pdf?v=1779759791\"\u003e41205_Manual_HB20260515\u003c\/a\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/40801ES-Manual-Ver.EN20250807.pdf?v=1772413651\"\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Yeasen","offers":[{"title":"2 T","offer_id":53544355987774,"sku":"41205ES02","price":105.0,"currency_code":"USD","in_stock":true},{"title":"10 T","offer_id":53544356020542,"sku":"41205ES10","price":405.0,"currency_code":"USD","in_stock":true},{"title":"20 T","offer_id":53544356053310,"sku":"41205ES20","price":745.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/universe_b9e9ebcb-b5e9-497a-b61d-dc198ade0f05.jpg?v=1754453675","url":"https:\/\/www.yeasenbio.com\/fa\/products\/41205","provider":"Yeasen - Leading Innovation in Molecular Enzymes and Reagents","version":"1.0","type":"link"}