{"product_id":"18565","title":"MolPure™ Magnetic Bacterial\/Fungal DNA Kit _ 18565ES","description":"\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eEffortless DNA extraction from Gram-negative bacteria, Gram-positive bacteria, and fungi.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eThe MolPure\u003c\/span\u003e™\u003cspan\u003e Magnetic Bacterial DNA Kit provides a simple and rapid solution for extracting genomic DNA from Gram-negative bacteria, Gram-positive bacteria, and fungi. The kit employs super-paramagnetic bead-based purification technology and requires no toxic phenol\/chloroform extraction. The workflow is straightforward, and the purified DNA is directly compatible with restriction digestion, PCR, sequencing, and labeling applications.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e• High yield and excellent purity: \u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003especific lysis beads provide more complete cell-wall disruption, yielding DNA of high purity and high recovery.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e• High efficiency:\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e lysozyme incubation is not required, and high-quality DNA can be obtained within a short hands-on time, enabling seamless integration with liquid-handling workstations or fully automated nucleic-acid extractors for rapid, large-scale processing.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e• Excellent safety: \u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003ethe workflow avoids toxic organic solvents including phenol, chloroform, and β-mercaptoethanol.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eApplications\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/1_a7cf2ed2-8cbd-4c14-b3f4-770df954a650_1024x1024.png?v=1787910200\" alt=\"Figure 1. DNA extraction from eight 7–8 ml bacterial cultures (centrifuged cell pellets), processed with 350 μl lysis buffer, 20 μl Proteinase K, and 10 μl RNase A, homogenized on a Retsch MM400 at 30 Hz for 5 min, incubated at 65°C for 10 min, and purified on a 48-channel automated extractor (70 μl elution). Gram-negative species (E. coli, Salmonella, Shigella) yielded Qubit concentrations \u0026amp;gt;120 ng\/μl; Gram-positive species (S. aureus, L. monocytogenes) were also successfully detected.\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eFigure 1. DNA extraction from eight 7–8 ml bacterial cultures (centrifuged cell pellets), processed with 350 μl lysis buffer, 20 μl Proteinase K, and 10 μl RNase A, homogenized on a Retsch MM400 at 30 Hz for 5 min, incubated at 65°C for 10 min, and purified on a 48-channel automated extractor (70 μl elution). Gram-negative species (E. coli, Salmonella, Shigella) yielded Qubit concentrations \u0026gt;120 ng\/μl; Gram-positive species (S. aureus, L. monocytogenes) were also successfully detected.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/2_be3c3486-595f-4f05-9256-07888ef781e5_1024x1024.png?v=1787910246\" alt=\"Figure 2. DNA extraction from six yeast cultures (350 μl lysis buffer, 20 μl Proteinase K, 10 μl RNase A; Retsch MM400 at 30 Hz for 5 min; 65°C for 10 min; 70 μL elution). The kit (18565ES) yields high concentrations with excellent purity, no RNA carry-over, and bright, sharp electrophoresis bands. Nanodrop A260\/A280 ratios: 1.79–1.95; A260\/A230 ratios: 1.71–1.84.\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003eFigure 2. DNA extraction from six yeast cultures (350 μl lysis buffer, 20 μl Proteinase K, 10 μl RNase A; Retsch MM400 at 30 Hz for 5 min; 65°C for 10 min; 70 μL elution). The kit (18565ES) yields high concentrations with excellent purity, no RNA carry-over, and bright, sharp electrophoresis bands. Nanodrop A260\/A280 ratios: 1.79–1.95; A260\/A230 ratios: 1.71–1.84.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003ePart I: shipped at room temperature and stored at 2 to 8°C; shelf life 18 months.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003ePart II: shipped at room temperature and stored at room temperature protected from light; shelf life 18 months.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Yeasen","offers":[{"title":"Default Title","offer_id":54345359360318,"sku":null,"price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/universe_b9e9ebcb-b5e9-497a-b61d-dc198ade0f05.jpg?v=1754453675","url":"https:\/\/www.yeasenbio.com\/fa\/products\/18565","provider":"Yeasen - Leading Innovation in Molecular Enzymes and Reagents","version":"1.0","type":"link"}