{"product_id":"16929","title":"Hifair™ CoAmp Multiplex One Step RT-qPCR Probe Kit（UDG Plus）_ 16929ES","description":"\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eThe Hifair™\u003c\/span\u003e\u003cspan\u003e CoAmp Multiplex One Step RT-qPCR Probe Kit (UDG Plus) is a multiplex one-step fluorescent quantitative PCR master mix designed for the efficient simultaneous detection of DNA and RNA targets within the same reaction system. By carefully integrating high-performance thermostable reverse transcriptase and hot-start DNA polymerase, along with a proprietary reaction buffer system, this kit achieves excellent amplification efficiency and balanced detection performance for both DNA and RNA templates. It effectively overcomes the amplification bias issues commonly encountered with traditional reagents during DNA\/RNA co-detection.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eThe core components of the kit include the deeply optimized 5× Hifair™\u003c\/span\u003e\u003cspan\u003e MP Buffer and a master mix enzyme system. The buffer contains sufficient Mg²⁺, dNTPs, and other essential components for amplification, and incorporates specific amplification enhancers and multiplex reaction stabilizers. This ensures high amplification efficiency while supporting robust multiplex detection of up to four targets. Additionally, the system incorporates dUTP and Uracil-DNA Glycosylase (UDG), which efficiently degrades carryover PCR products, fundamentally preventing amplification product contamination and ensuring the reliability and reproducibility of experimental results.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eFeature\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003es\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003eDual-Detection, Doubled Throughput\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e: Simultaneous DNA \u0026amp; RNA amplification in a single tube saves 50% of reaction wells and doubles testing capacity.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e\u003cb\u003e\u003cspan\u003eRapid Results:\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e Delivers both DNA and RNA pathogen results in a single reaction, accelerating clinical and veterinary decision-making.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e\u003cb\u003e\u003cspan\u003eVersatile \u0026amp; Cost-Effective: \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003eCompatible with both DNA and RNA assays, streamlining production and reducing costs for large-scale manufacturing.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e\u003cb\u003e\u003cspan\u003eHigh Performance \u0026amp; Stability:\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e Achieves a sensitivity of 250 copies\/mL, remains stable after 14 days at 37°C, supports rapid cycling, and is compatible with all major real-time PCR instruments.\u003c\/span\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"19.3600%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"23.1800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"13.8200%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e16929ES50\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e（\u003c\/span\u003e50 T\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e）\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"13.8800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e16929ES60\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e（\u003c\/span\u003e100 T\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e）\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"14.1800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e16929ES80\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e（\u003c\/span\u003e1,000 T\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e）\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"15.5200%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e16929ES92\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e（\u003c\/span\u003e10,000 T\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e）\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"19.3600%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e16929-A\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"23.1800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e5×Hifair™\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eMP Buffer \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"13.8200%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e250 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"13.8800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e500 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"14.1800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e5 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"15.5200%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e50 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"19.3600%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e16929-\u003c\/span\u003e\u003cspan\u003eB\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"23.1800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHifair™\u003c\/span\u003e\u003cspan\u003e Enzyme Mix\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"13.8200%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e50 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"13.8800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e100 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"14.1800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"15.5200%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cb\u003e\u003cspan\u003e[Note]\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e:\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e1. 1.5× Hifair™\u003c\/span\u003e\u003cspan\u003e MP Buffer is an abbreviation for Hifair™\u003c\/span\u003e\u003cspan\u003e Multiplex One Step RT-qPCR Probe Kit (UDG Plus).\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e2. Hifair™\u003c\/span\u003e\u003cspan\u003e Enzyme Mix mainly contains thermostable Reverse Transcriptase and HotStart Taq DNA Polymerase, RNase inhibitor, UDGase, etc.\u003c\/span\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eStorage\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThis product should be stored at -25~-15℃ for 1 year.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eApplication\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003eCo-detection of DNA \u0026amp; RNA Pathogen. Pathogen Multiplex Assay\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eFigures\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e1. \u003c!--[endif]--\u003eEfficient Single-Tube DNA\/RNA Co-amplification  \u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\n\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/1_88b4ec08-0b40-4dc1-86da-02b9e6554cb2_1024x1024.png?v=1781225730\" alt=\"Figure 1. Co-detection of DNA and RNA pathogens.\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003cspan\u003e \u003c\/span\u003eFigure 1. Co-detection of DNA and RNA pathogens.\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/div\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cbr\u003eUsing Reagent 16929, the simultaneous detection of African Swine Fever Virus (DNA) and Porcine Reproductive and Respiratory Syndrome Virus (RNA) was performed in a single reaction well. Results demonstrate that both targets achieved amplification efficiencies exceeding 90%, with no amplification bias, ensuring balanced and reliable outcomes.\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003c!-- [if !supportLists]--\u003e2. \u003c!--[endif]--\u003eStrong Stability\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/2_04a36714-aa65-4cd7-85ec-4f1376b2c27f_1024x1024.png?v=1781225730\" alt=\"Figure 2. Stability assessment\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp class=\"p\" style=\"text-align: center;\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003eFigure 2. Stability assessment\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan class=\"15\"\u003e(A) Accelerated Stability: After incubation at 37°C for 14 days, Reagent 16929 showed no changes in detection rate, Ct values, or peak shape compared to the -20°C control, demonstrating excellent thermal stability.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan class=\"15\"\u003e(B) Freeze-Thaw Stability: The reagent maintained consistent amplification performance for both DNA-HPIV and RNA-HCOV after 50 dry ice freeze-thaw cycles.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"p\"\u003e\n\u003cspan\u003eDocuments:\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp\u003eSafety Data Sheet\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/16929-MSDS-HB260609.pdf?v=1781225081\"\u003e16929_MSDS_HB260609\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eManuals\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/16929-Manual-Ver.EN20260609.pdf?v=1781225081\"\u003e16929_Manual_HB20260609\u003c\/a\u003e\u003c\/p\u003e","brand":"Yeasen","offers":[{"title":"50 T","offer_id":53732737155390,"sku":"16929ES50","price":85.0,"currency_code":"USD","in_stock":true},{"title":"100 T","offer_id":53732737188158,"sku":"16929ES60","price":125.0,"currency_code":"USD","in_stock":true},{"title":"1000 T","offer_id":53732737220926,"sku":"16929ES80","price":1095.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/universe_b9e9ebcb-b5e9-497a-b61d-dc198ade0f05.jpg?v=1754453675","url":"https:\/\/www.yeasenbio.com\/fa\/products\/16929","provider":"Yeasen - Leading Innovation in Molecular Enzymes and Reagents","version":"1.0","type":"link"}