{"product_id":"14325","title":"Hieff UNICON™ Robust Hotstart Taq DNA Polymerase (20 U\/μL）_ 14325ES","description":"\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eThis product is a hot-start DNA polymerase that utilizes two proprietary antibodies for dual blocking. It effectively inhibits both the 5'→3' polymerase activity and the 5'→3' exonuclease activity of Taq DNA polymerase. Upon heating at the pre-denaturation temperature for 30 seconds, the blocking antibodies are completely inactivated, releasing both the DNA polymerase and exonuclease activities.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eThis dual-blocking feature offers a dual advantage: it not only prevents non-specific amplification caused by mismatches or primer-dimers but also inhibits the degradation of probes that can lead to a decrease in fluorescent signal. This dual protection ensures greater stability of in vitro diagnostic reagents during transportation or when used at room temperature.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eFeature\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003es\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli align=\"justify\"\u003e\n\u003cstrong\u003eAntibody-mediated hot start: \u003c\/strong\u003eActivated by a brief 95 \u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e°\u003c\/span\u003e\u003cspan style=\"font-family: Inter;\"\u003eC incubation (30\u003c\/span\u003e\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e–\u003c\/span\u003e\u003cspan style=\"font-family: Inter;\"\u003e60 s) for precise reaction control.\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli align=\"justify\"\u003e\n\u003cstrong\u003eHigh sensitivity \u0026amp; specificity:\u003c\/strong\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter;\"\u003e \u003c\/span\u003e\u003c\/span\u003eReliable target detection with minimal non-specific amplification.\u003cspan class=\"15\"\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli align=\"justify\"\u003e\n\u003cstrong\u003eInhibitor tolerant: \u003c\/strong\u003ePerforms well in the presence of common inhibitors from blood, swabs, and residual prep reagents (e.g., phenol, alcohol).\u003cspan class=\"15\"\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"139\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"207\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"125\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e14325\u003c\/span\u003e\u003cspan\u003eES\u003c\/span\u003e\u003cspan\u003e76\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e(1,00\u003c\/span\u003e\u003cspan\u003e0 \u003c\/span\u003e\u003cspan\u003eU)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"126\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e14325\u003c\/span\u003e\u003cspan\u003eES\u003c\/span\u003e\u003cspan\u003e80\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e(10,000\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eU)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"126\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e14325\u003c\/span\u003e\u003cspan\u003eES\u003c\/span\u003e\u003cspan\u003e92\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e(25,000\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eU)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"126\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e14325\u003c\/span\u003e\u003cspan\u003eES\u003c\/span\u003e\u003cspan\u003e93\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e(100,000 U)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"139\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e14325\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"207\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eRobust \u003c\/span\u003e\u003cspan\u003eHotstart Taq (\u003c\/span\u003e\u003cspan\u003e20 U\u003c\/span\u003e\u003cspan\u003e\/μL)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"125\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e50 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"126\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e500 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"126\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1.25 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"126\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e5 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cbr\u003e\n\u003c\/h3\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eStorage\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThis product should be stored at -25~-15℃ for 2 years.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"p\"\u003e\n\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eFigure\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cb\u003e\u003cspan\u003e1. Direct Amplification from Swab Samples\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cspan\u003eNasopharyngeal swabs were vortexed in a direct lysis buffer or diluted in TE buffer to prepare templates at equivalent concentrations. Both were tested using the 14325 qPCR master mix and a leading commercial alternative. Results showed that the 14325 formulation exhibits superior inhibitor tolerance, enabling reliable direct amplification from swab samples without purification.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/1_e78fa180-369f-47cd-b0ba-d877cf85ee3f_1024x1024.png?v=1773107755\" alt=\"Figure 1. Comparison of direct swab amplification performance: 14325 vs. commercial reagents.\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp class=\"p\" align=\"justify\" style=\"text-align: center;\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003cb\u003e\u003cspan\u003eFigure 1. Comparison of direct swab amplification performance: 14325 vs. commercial \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003ereagents\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cb\u003e\u003cspan\u003e2. Direct Amplification from Plasma Samples\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cspan\u003eClinical plasma samples were diluted in either plasma itself or TE buffer to match template concentrations. When tested with the 14325 qPCR mix and a commercial \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003ereagent\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e, the 14325 system demonstrated significantly better resistance to inhibitors—supporting direct amplification with up to 30% final plasma concentration.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/2_d9b2f1ab-d5cd-455b-a8c5-db40d705ff01_1024x1024.png?v=1773107765\" alt=\"Figure 2. Comparison of direct plasma amplification performance: 14325 vs. commercial reagent.\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp class=\"p\" align=\"justify\" style=\"text-align: center;\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003cb\u003e\u003cspan\u003eFigure 2. Comparison of direct plasma amplification performance: 14325 vs. commercial \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003ereagent\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ch3 class=\"p\"\u003e\n\u003cspan\u003eDocuments:\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp\u003eSafety Data Sheet\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/14325-MSDS-HB260304.pdf?v=1773107583\"\u003e14325_MSDS_HB260304_EN.PDF\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eManuals\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/14325-Manual-Ver.EN20260302.pdf?v=1773107585\"\u003e14325_Manual_Ver.EN20260304.pdf\u003c\/a\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e","brand":"Yeasen","offers":[{"title":"1000U","offer_id":53114289815870,"sku":"14325ES76","price":205.0,"currency_code":"USD","in_stock":true},{"title":"10000U","offer_id":53114289848638,"sku":"14325ES80","price":1765.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/14325ES.jpg?v=1773108093","url":"https:\/\/www.yeasenbio.com\/fa\/products\/14325","provider":"Yeasen - Leading Innovation in Molecular Enzymes and Reagents","version":"1.0","type":"link"}