{"product_id":"10410","title":"Proteinase K Solution (20 mg\/mL, Low DNA,  mNGS， RNase-Free, DNase-Free) _ 10410ES","description":"\u003cp class=\"MsoNormal\"\u003e\u003ca name=\"OLE_LINK23\"\u003e\u003c\/a\u003e\u003cmeta charset=\"utf-8\"\u003e\u003c\/p\u003e\n\u003cp dir=\"auto\"\u003eProteinase K is a broad-specificity serine protease with a relative molecular weight of approximately 29.3 kDa. It cleaves peptide bonds at the carboxyl side of aliphatic and aromatic amino acids. Proteinase K has wide applications, including preparation of high-molecular-weight chromosomal DNA for pulsed-field gel electrophoresis, Western blotting (protein immunoblotting), and removal of nucleases during DNA and RNA preparations. Denaturants such as SDS (1%) can enhance its activity. The typical working concentration is 50–100 µg\/mL, with the exact concentration determined based on factors such as the presence of SDS or urea in the buffer, pH, and temperature.\u003c\/p\u003e\n\u003cp dir=\"auto\"\u003eThis product is a \u003cspan style=\"color: rgb(255, 42, 0);\"\u003e\u003cstrong\u003elow-residual Proteinase K\u003c\/strong\u003e\u003c\/span\u003e, which maintains the enzyme's inherent properties while featuring significantly reduced background microbial residues, ensuring greater accuracy in downstream detection applications.\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eDerived from \u003cem\u003eTritirachium album\u003c\/em\u003e (also known as \u003cem\u003eParengyodontium album\u003c\/em\u003e or \u003cem\u003eLimber\u003c\/em\u003e strain in some references);\u003c\/li\u003e\n\u003cli\u003eSpecific activity: ≥800 U\/mL;\u003c\/li\u003e\n\u003cli\u003eProtein concentration: ≥20 mg\/mL;\u003c\/li\u003e\n\u003cli\u003eFree of RNase and DNase contamination;\u003c\/li\u003e\n\u003cli\u003eStable activity in the presence of urea and SDS;\u003c\/li\u003e\n\u003cli\u003eActive over a broad pH range (pH 4.5–12.5), with optimal activity between pH 7.0–12.0;\u003c\/li\u003e\n\u003cli\u003eReaction conditions: Incubate in reaction buffer at 37°C–65°C for 1 hour. Recommended temperature: 65°C;\u003c\/li\u003e\n\u003cli\u003eUnit definition: One unit is the amount of enzyme required to hydrolyze casein to release 1 µmol of L-tyrosine per minute under defined conditions;\u003c\/li\u003e\n\u003cli\u003eInactivation method: Add PMSF or DFIP inhibitor to the reaction system for complete inactivation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003eApplications\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eNucleic Acid Extraction \u0026amp; Purification\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eRemove endogenous nucleases during the preparation of DNA and RNA\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003ePrepare tissue sections for in - situ hybridization.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003eSpecifications\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"259\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eCat.No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"542\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10410ES01 \/ 10410ES05 \/ 10410ES60 \/10410ES70\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"259\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSize\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"542\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1 mL \/5 mL\/ 100 mL \/1000 mL \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003eShipping and Storage\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThis product should be stored at 2~8℃ for 2 years.For longer storage, it can be stored at -25~-15℃.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003eDocuments:\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10410-MSDS-HB2603181.pdf?v=1773822890\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10410-Manual-Ver.EN20250617_9c254a29-2a76-4ba0-9825-3e1b829669e1.pdf?v=1773822899\"\u003eManual\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10410-P0531031_CoA-EN.pdf?v=1774334828\"\u003eCOA\u003c\/a\u003e\u003c\/p\u003e\n\u003ch3\u003eRelated Blog\u003c\/h3\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeasenbio.com\/blogs\/molecular-biology\/low-dna-proteinase-k-eliminate-background-noise-in-pathogen-detection\"\u003eLow-DNA Proteinase K: Eliminate Background Noise in Pathogen Detection\u003c\/a\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10412-Manual-Ver.EN20250617.pdf?v=1750321200\"\u003e\u003cspan\u003e\u003cu\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/u\u003e\u003c\/span\u003e\u003c\/a\u003e\u003ca name=\"OLE_LINK24\"\u003e\u003c\/a\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Yeasen","offers":[{"title":"1 mL","offer_id":53186760933694,"sku":"10410ES01","price":65.0,"currency_code":"USD","in_stock":true},{"title":"5 mL","offer_id":53186760966462,"sku":"10410ES05","price":105.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":53186760999230,"sku":"10410ES60","price":1055.0,"currency_code":"USD","in_stock":true},{"title":"1000 mL","offer_id":53186761031998,"sku":"10410ES70","price":8405.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/12316_788b5de8-bdeb-4cce-854a-38ace1cb6c15.jpg?v=1739429378","url":"https:\/\/www.yeasenbio.com\/fa\/products\/10410","provider":"Yeasen - Leading Innovation in Molecular Enzymes and Reagents","version":"1.0","type":"link"}