{"product_id":"16914","title":"Hifair™ SmartApt Ⅰ One Step RT-qPCR Probe Master Mix (One-tube, 3G) _ 16914ES","description":"\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThis product is a single-tube, probe-based RT-qPCR master mix specifically formulated for one-step quantitative PCR assays using RNA templates, suitable for both singleplex and multiplex detection. Reverse transcription and qPCR are performed in the same reaction tube, simplifying workflow and minimizing the risk of contamination.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThe kit incorporates a next-generation antibody-mediated hot-start Taq DNA polymerase, aptamer-modified reverse transcriptase, and a specialized buffer system optimized for one-step, fully pre-mixed RT-qPCR. It supports pre-mixing of primers and probes; users only need to add template RNA during setup, thereby reducing handling steps and reagent loss. Additionally, the formulation includes a dUTP\/UDG system that remains active at room temperature, effectively mitigating the risk of aerosol-mediated carryover contamination.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThe aptamer-modified reverse transcriptase ensures near-complete suppression of enzymatic activity at low or ambient temperatures while enhancing overall enzyme stability. Validation studies demonstrate that primer\/probe pre-mixed reactions remain stable—showing no performance degradation—after storage at 25°C for 2 weeks or at 4°C for 4 weeks. Therefore, accelerated stability testing is recommended at temperatures such as 25°C, 16°C, and 4°C to best reflect real-world product performance.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eExceptional Premix Stability\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e: Support prime\u0026amp;probe premixing, with proven stability at 37°C for up to 5 days.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eSupports fast protocols: \u003c\/span\u003e\u003c\/b\u003eResults available in as little as \u003cspan style=\"font-family: Inter Medium;\"\u003e3\u003c\/span\u003e0 minutes.\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eUltra-High Sensitivity and Specificity:\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e Features a genetically engineered antibody-based hot-start Taq polymerase for enhanced sensitivity and specificity.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003edUTP\/UDG Anti-Contamination System\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e: Incorporates a dUTP\/UDG system to effectively prevent aerosol contamination.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eBroad Instrument Compatibility\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e: Compatible with a wide range of real-time PCR platforms, including Bio-Rad CFX96, ABI Q5, 7500, and SLAN.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"40.4400%\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"14.8400%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003e16914ES50 \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003e(50 T)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"14.9000%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003e16914ES60 \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003e(100 T)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"14.9000%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003e16914ES80\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003e (1,000 T)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"14.8400%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003e16914ES92 \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003e(10,000 T)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"40.4400%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e2× Hifair\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eTM\u003c\/span\u003e\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e SmartApt I Master Mix\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"14.8400%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e625 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"14.9000%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1.25 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"14.9000%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e12.5 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"14.8400%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e125 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cb style=\"font-size: 0.875rem;\"\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e[\u003c\/span\u003e\u003c\/b\u003e\u003cb style=\"font-size: 0.875rem;\"\u003eNote\u003c\/b\u003e\u003cb style=\"font-size: 0.875rem;\"\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e]\u003c\/span\u003e\u003c\/b\u003e\u003cb style=\"font-size: 0.875rem;\"\u003e: \u003c\/b\u003e“2× HifairTM SmartApt I Master Mix” is the abbreviated name for HifairTM SmartApt I One Step RT-qPCR Probe Master Mix, which contains dNTPs, dUTP, Mg²⁺, stabilizers, enhancers, reverse transcriptase, hot-start Taq DNA polymerase, and UDGase.\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eStorage\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp\u003eThis product should be stored at -25~-15℃ for \u003cspan style=\"font-family: Inter Medium;\"\u003e1\u003c\/span\u003e year.\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cstrong\u003eApplication\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan class=\"15\"\u003eUrgent testing (e.g., Infectious disease detection, Critical care diagnostics);\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan class=\"15\"\u003eHigh-throughput testing(e.g., Disease screening, Pathogen screening, Virus detection);\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan class=\"15\"\u003eRoutine testin(e.g., Food microbiology detection ).\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eFigures\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e\u003cstrong\u003e1.\u003c\/strong\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eHigh Seneitivity \u0026amp; Superior fully premixed stability \u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4\u003e\u003cspan class=\"15\"\u003e \u003c\/span\u003e\u003c\/h4\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/29ec6dc6c7d6ded07b927fb15c6d5ed1_1024x1024.png?v=1769751601\" alt=\"Figure 1. Low-concentration detection performance of fully premixed 16914ES.\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eFig\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eure 1\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e. Low-concentration detection performance of fully premixed 1691\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e4\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eES.\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cspan class=\"15\"\u003eHPIV and RSV pseudovirus templates (1 copy\/μL) were tested using a 25 μL respiratory assay. The 1691\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e4\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"15\"\u003eES mix premixed with primers and probes was stored at 37°C for 5 days and compared with –20°C and freshly prepared controls. The amplification curves, fluorescence signals, Ct values, and detection rates showed no noticeable changes, confirming the strong stability and sensitivity of the fully premixed 1691\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e4\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"15\"\u003eES.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"p\"\u003e\n\u003cstrong\u003eDocuments:\u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSafety Data Sheet\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/16914-MSDS-HB251211.pdf?v=1769750567\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e16914\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e_MSDS_HB\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e60130\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e_EN.PDF\u003c\/span\u003e\u003c\/a\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eManuals\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/16914ES-Manual-Ver.EN20251210.pdf?v=1769750564\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e16914\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e_Manual_Ver.E\u003c\/span\u003e\u003cspan\u003eN\u003c\/span\u003e\u003cspan\u003e202\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e60130\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e.pdf\u003c\/span\u003e\u003c\/a\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Yeasen","offers":[{"title":"50 T","offer_id":52946609242430,"sku":"16914ES50","price":205.0,"currency_code":"USD","in_stock":true},{"title":"100 T","offer_id":52946609275198,"sku":"16914ES60","price":335.0,"currency_code":"USD","in_stock":true},{"title":"1000 T","offer_id":52946609307966,"sku":"16914ES80","price":2345.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/5f6f2605b13c7160f2b137d6dd332492_748fef5e-3bfc-4574-bcad-37af5d459745.webp?v=1769752724","url":"https:\/\/www.yeasenbio.com\/es\/products\/16914","provider":"Yeasen - Leading Innovation in Molecular Enzymes and Reagents","version":"1.0","type":"link"}