{"product_id":"10930","title":"Hieff™ Clone Quick T4 Ligation Mix _ 10930ES","description":"\u003cp\u003eHieff™ Clone Quick T4 Ligation Mix is a ready-to-use 2× premix for cloning. It is simple to operate, enabling ligation of insert fragments with vectors at 25°C for 5 minutes and saving experimental time. This kit is suitable not only for sticky-end ligation, but also compatible with blunt-end ligation and TA ligation. In addition, the reagent maintains stable reaction performance even after repeated freeze–thaw cycles.\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eProduct Information\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoNormalTable\" border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"481\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eCatalog No.\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"481\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSize\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"481\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10930ES20 \/ 10930ES60\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"481\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e20 T \/ 100 T\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eComponent Information\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoNormalTable\" border=\"0\" cellspacing=\"0\" style=\"width: 99.9376%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"353\" valign=\"center\" style=\"width: 54.0074%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eComponent Name\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"303\" valign=\"center\" style=\"width: 32.4762%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003e10930ES20\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"328\" valign=\"center\" style=\"width: 13.457%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003e10930ES60\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"353\" valign=\"center\" style=\"width: 54.0074%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e2× Hieff Clone™ Quick T4 Ligation Mix\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"303\" valign=\"center\" style=\"width: 32.4762%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e100 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"328\" valign=\"center\" style=\"width: 13.457%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e2×250 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eFeature\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eFast: 5-minute quick ligation\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eEfficient: high cloning positive rate\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eApplications\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e1. Size-fragment compatibility, 5 min efficient ligation\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/2_f86d6fc8-3abd-4d30-88bb-04ddcb82d270_1024x1024.png?v=1789024479\" alt=\"Figure 1: At 25°C for 5 min, 10930ES rapidly and efficiently ligated 100 bp, 1 kb, 4 kb, and 5 kb fragments, with a 100% positive rate for each fragment length. Top: transformation plate results; bottom: colony PCR identification electrophoresis.\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003eFigure 1: At 25°C for 5 min, 10930ES rapidly and efficiently ligated 100 bp, 1 kb, 4 kb, and 5 kb fragments, with a 100% positive rate for each fragment length. Top: transformation plate results; bottom: colony PCR identification electrophoresis.\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e2. Excellent stability\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/3_dfb78a1f-c96e-4ee4-ae3f-3114dac9a199_1024x1024.png?v=1789024431\" alt=\"Figure 2: After storage at 25°C for 28 days and 40 freeze–thaw cycles, 10930ES still stably ligated 600 bp fragments, with a 100% positive rate. Top: transformation plate results (left: 40 freeze–thaw cycles; right: 25°C for 28 days); bottom: colony PCR identification electrophoresis.\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\" style=\"text-align: center;\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003cb\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003eFigure 2: After storage at 25°C for 28 days and 40 freeze–thaw cycles, 10930ES still stably ligated 600 bp fragments, with a 100% positive rate. Top: transformation plate results (left: 40 freeze–thaw cycles; right: 25°C for 28 days); bottom: colony PCR identification electrophoresis.\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Yeasen","offers":[{"title":"20 T","offer_id":54427047100734,"sku":"10930ES20","price":60.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":54427047133502,"sku":"10930ES60","price":220.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/5abda97db9c371a240cf9285d74a3035_f023c276-6e82-49a0-8644-09181f809df8.jpg?v=1790645491","url":"https:\/\/www.yeasenbio.com\/da\/products\/10930","provider":"Yeasen - Leading Innovation in Molecular Enzymes and Reagents","version":"1.0","type":"link"}