The Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase is one of the commonly used reverse transcriptases in molecular biology laboratory procedures. To achieve higher reverse transcription efficiency, better thermal stability, and an increased rate of cDNA synthesis, the original reverse transcriptase is often genetically engineered. Currently, the reverse transcription reagents available on the market typically require reaction times ranging from 15 to 30 minutes, which is relatively long. When the number of reaction samples is large, the overall time expenditure can increase dramatically. YESSEN existing 5-minute reverse transcription series products can complete the reverse transcription process within just 5 minutes, saving time and being highly efficient.

Boutique Recommendation One

Rapid, Efficient, and High-Yield Reverse Transcription Premix (with gDNA Removal)(Cat #11155ES)

High reverse transcription yield from different templates

Figure. Reverse transcription was performed using RNA templates from human 293T cells, Arabidopsis thaliana, mouse liver, and fish liver. Brands A, B, C, and D are all two-step digestion reverse transcription reagents with varying reverse transcription times from 15 to 20 minutes. The test results show that the Hifair 11155ES 5-minute reverse transcription kit excels with higher yields compared to other brands, and the Ct values are lower.

Boutique Recommendation Two

5-Minute One-Step Digestion & Reverse Transcription Premix(Cat #11151ES)

High reverse transcription yield

Figure. Using human 293T cell RNA as the template, three genes were tested respectively. Brands A, B, and C are all one-step gDNA removal & reverse transcription reagents. The test results show that with an input of 1 μg and 10 ng of Total RNA, the yield of reverse transcription with the 11151ES kit is superior to other brands, with smaller Ct values.

Figure. Using Arabidopsis thaliana RNA as the template, Brands A, B, and C are all one-step gDNA removal & reverse transcription reagents. The test results show that with an input of 1 μg and 10 ng of Total RNA, the yield of reverse transcription with the 11151ES kit is superior to other brands, with smaller Ct values.

Excellent sensitivity

Figure. Using human 293T cell RNA as the template, genes with 30% GC, 50% GC, 60% GC, and 70% GC content were amplified. Total RNA inputs of 100 pg, 1 ng, 10 ng, 100 ng, and 1 μg were used for reverse transcription followed by fluorescent quantitative detection. The results show that 11151ES can accommodate reverse transcription for RNA inputs ranging from 100 pg to 1 μg.

Boutique Recommendation Three

5-Minute Rapid Reverse Transcription Kit(Cat #11149/11150ES)

High sensitivity, with a minimum detection limit down to 10 pg

Figure. Using total RNA from 293T cells as the template, reverse transcription was performed with 11149ES, and the resulting cDNA was subjected to qPCR quantitative analysis. The results show that 11149ES can successfully reverse transcribe RNA inputs ranging from 10 pg to 1 μg, ensuring accurate quantitative results.

Good amplification performance, with the longest amplification of a 14 kb fragment

Figure. Using total RNA from 293T cells as the template, reverse transcription was performed with 11149ES, and the resulting cDNA was subjected to PCR detection. The results indicate that 11149ES is capable of synthesizing cDNA fragments ranging from 1 kb to 14 kb in length, with subsequent PCR having certain applicable scenarios. If direct reverse transcription of fragments over 10 kb is required, it is recommended to extend the reverse transcription time to 20-30 minutes to ensure the acquisition of the final fragments.

Comparison of reverse transcription efficiency

Figure. Using total RNA from 293T cells as the template, reverse transcription was performed with 11149ES and Brand A, with 11149ES not undergoing the gDNA removal step. The resulting cDNA was subjected to qPCR quantitative analysis. The results show that the reverse transcription time for Brand A was 13 minutes, while for 11149ES it was 5 minutes. The quantitative analysis of different products reveals that the Ct values for 11149ES are smaller or similar to those of the competitive brand, indicating that the 5-minute reverse transcription efficiency of 11149ES is superior to or approximately equivalent to that of Brand A.

Figure. Using total RNA from 293T cells as the template, reverse transcription was performed with 11149ES and Brand B, with 11149ES not undergoing the gDNA removal step. The resulting cDNA was subjected to qPCR quantitative analysis. The results show that the reverse transcription time for Brand B was 20 minutes, while for 11149ES it was 5 minutes. The quantitative analysis of different substrate quantities reveals that the Ct values for 11149ES are smaller or similar to those of Brand B, indicating that the 5-minute reverse transcription efficiency of 11149ES is superior to or approximately equivalent to that of Brand B.

Recommended related products