Timentin - A New Type of Plant Tissue Culture Antibiotic as an Agrobacterium Inhibitor

1 Application of Timentin

Plant tissue culture is widely used in plant research, such as genetic engineering, model plants, and crop breeding. Contamination of seedlings during tissue culture is a very troublesome issue, among which Agrobacterium is a common Gram-negative bacterium found in soil. It can infect the injured parts of most dicotyledonous and gymnosperm plants under natural conditions. Cells at the injured sites secrete a large amount of phenolic compounds, which attract Agrobacterium to these cells and induce the formation of crown galls or hairy roots.

Timentin is a new type of antibiotic that effectively inhibits Agrobacterium.

2 Principle of Action of Timentin

Timentin has a broad spectrum of antibacterial activity against Gram-positive and negative bacteria, as well as aerobic and anaerobic bacteria. Its components are Ticarcillin Sodium and Clavulanate Potassium, with a ratio of 15:1 based on the effective acid content. Ticarcillin is a penicillin-like bactericidal agent, while Clavulanate is an irreversible and highly effective β-lactamase inhibitor.

Many Gram-positive (G+) and negative (G-) bacteria can produce β-lactamase, an enzyme that can destroy penicillin before it acts on the pathogen. Clavulanate blocks the action of β-lactamase, breaking down the bacterial defense barrier and restoring the sensitivity of Ticarcillin. Clavulanate Potassium has minimal antibacterial activity on its own, but when combined with Ticarcillin, it makes the product a broad-spectrum bactericidal antibiotic suitable for empirical treatment of a wide range of bacterial infections.

3 Advantages of Timentin

  • Minimal impact on plant materials: Particularly minimal impact on the formation of callus tissue and plant regeneration.
  • Suitable for the transformation process of difficult-to-transform materials: Timentin can be used when plants are difficult to inhibit with other antibiotics.
  • Higher efficiency in inhibiting Agrobacterium: Timentin is more efficient in inhibiting common plant Agrobacterium than carbenicillin and cephalothin.

4 Preparation Method (for reference only)

  • Usage concentration: In plant tissue culture, the recommended usage concentration is 200-400 mg/mL.
  • Culture medium preparation: Newly prepared solid culture medium should be sterilized at high temperature and pressure at 121°C for 15-20 minutes. After sterilization, remove the culture medium (if it is pre-prepared solid culture medium, it can be heated and dissolved in a microwave oven first). When the culture medium cools to about 50°C, add 0.1% Timentin stock solution (v/v) in a laminar flow cabinet, mix well, and then pour the culture medium into sterile culture vessels for later use.

5 Product Recommendation

Yeasen Biotech offers high-quality Timentin with ample stock available.

Product name

Cat#

Specification

Timentin

60230ES07/60

3.2 g/100 g

 

6 Related Products

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Hygromycin B

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Timentin

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7 Yeasen's series of antibiotics products have been cited in publications (with a cumulative impact factor of over 1000)

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[3] Zhang D, Liu Y, Zhu Y, et al. A non-canonical cGAS-STING-PERK pathway facilitates the translational program critical for senescence and organ fibrosis[J].Nat Cell Biol. 2022;24(5):766-782. doi:10.1038/s41556-022-00894-z.IF:28.824

[4] Lu T, Zhang Z, Zhang J, et al. CD73 in small extracellular vesicles derived from HNSCC defines tumour-associated immunosuppression mediated by macrophages in the microenvironment[J].J Extracell Vesicles. 2022;11(5):e12218. doi:10.1002/jev2.12218.IF:25.841

[5] Meng F, Yu Z, Zhang D, et al. Induced phase separation of mutant NF2 imprisons the cGAS-STING machinery to abrogate antitumor immunity[J]. Mol Cell. 2021;81(20):4147-4164.e7. doi:10.1016/j.molcel.2021.07.040.IF:17.970

[6] Fansen Meng, Zhengyang Yu, Dan Zhang,et al.Induced phase separation of mutant NF2 imprisons the cGAS-STING machinery to abrogate antitumor immunity[J].MOLECULAR CELL.2021 Oct;81:4147.IF=17.97

[7] Xueping Hu, Jinping Pang, Jintu Zhang,et al.Discovery of Novel GR Ligands toward Druggable GR Antagonist Conformations Identified by MD Simulations and Markov State Model Analysis[J].Advanced Science.2022 Jan;9(3):2102435.IF=16.806

[8] Cefan Zhou,Yanyan Liang ,Li Zhou,et al.TSPAN1 promotes autophagy flux and mediates cooperation between WNT-CTNNB1 signaling and autophagy via the MIR454-FAM83A-TSPAN1 axis in pancreatic cancer[J]. Autophagy. 2021;17(10):3175-3195.IF=16.016

[9] Jun Qin, Jian Zou, Zhengfan Jiang,et al.TBK1-Mediated DRP1 Targeting Confers Nucleic Acid Sensing to Reprogram Mitochondrial Dynamics and Physiology[J].MOLECULAR CELL.2020 Dec;80:810.IF=15.584

[10] Shuai Jin, Hongyuan Fei, Zixu Zhu,et al.Rationally Designed APOBEC3B Cytosine Base Editors with Improved Specificity[J].MOLECULAR CELL.2020 Sep;79:728.IF=15.584