{"title":"mRNA Enzyme","description":"\u003cp dir=\"auto\"\u003eIn vitro mRNA synthesis produces mRNA for vaccines and gene therapy. A linearized DNA template with a promoter (e.g., T7 promoter:\u003cspan\u003e 5'-TAATACGACTCACTATAG-3'\u003c\/span\u003e\u003cspan data-cid=\"38ad740e-5474-4910-bee4-91c0e4cfaa50\" class=\"pjBG2e\"\u003e\u003cspan class=\"UV3uM\"\u003e \u003c\/span\u003e\u003c\/span\u003e) is transcribed by T7 RNA polymerase using nucleotides, buffer, and magnesium ions. A 5' cap is added via a capping enzyme or cap analog, and a poly-A tail is included using the template or poly-A polymerase for stability. DNase I removes residual DNA, and the mRNA is purified. Key enzymes—BspQ1, T7 RNA polymerase, Pyrophosphatase, Inorganic, Murine RNase inhibitor, capping enzyme, poly-A polymerase, and DNase I—ensure functional mRNA.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeasenbio.com\/collections\/biopharma-product-selection\"\u003eProduct Selection Guide\u003c\/a\u003e\u003c\/p\u003e","products":[{"product_id":"14615","title":"RNase R  (GMP-grade, 20 U\/μL) _ 14615ES","description":"\u003cp style=\"font-weight: 400;\"\u003eRibonuclease R (RNase R) from\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e, is a magnesium-dependent 3′→5′ exoribonuclease that\u003cspan\u003e \u003c\/span\u003ecan digest all linear RNAs but does not digest lariat or circular RNA structures. Most cellular RNAs will be digested completely by RNase R, with the exception of tRNAs, 5S RNA and intron lariats. RNase R is used for enrichment of circRNAs in biological samples, identification of intronic lariat sequences, identification of exonic circRNAs, studying alternative splicing.\u003c\/p\u003e\n\u003carticle class=\"4ever-article\"\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003e\u003cstrong\u003e\u003cspan data-type=\"text\"\u003e\u003cspan data-type=\"leaf\"\u003eRNase R must be used in combination with the 10× RNase R Reaction Buffer, GMP-grade (Cat. No. 14616ES)\u003c\/span\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/article\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong data-filtered=\"filtered\"\u003eFeatures  \u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"font-weight: 400;\"\u003eGuaranteed efficacy and quality with ISO 13485 certification.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eAvailable in bulk or tailored configurations.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eEffectively breaks down linear RNA, while preserving lariat loops and circular RNAs.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eSelective digestion of linear RNAs enhances the isolation of circular RNAs for protein production or intronic cDNA library construction.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eOffering GMP-Grade RNAse R\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong data-filtered=\"filtered\"\u003eSpecificntions\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable style=\"font-weight: 400;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eSource\u003c\/td\u003e\n\u003ctd\u003eA recombinant \u003cem\u003eE. coli\u003c\/em\u003e strain carrying the RNAse R gene from \u003cem\u003eE. coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOptimum Temperature\u003c\/td\u003e\n\u003ctd\u003e37℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage Buffer\u003c\/td\u003e\n\u003ctd\u003e50 mM Tris-HCl,100 mM NaCl, 0.1 mM EDTA, 0.1% Triton® X-100, 1 mM DTT, 50% glycerol，pH7.5 at 25℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eUnit Definition\u003c\/td\u003e\n\u003ctd\u003eOne unit converts 1 μg of poly-r(A) into acid-soluble nucleotides in 10 minutes at 37°C in 20 mM Tris-HCl (pH 8.0), 100 mM KCl and 0.1 mM MgCl\u003csub\u003e2\u003c\/sub\u003e.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong data-filtered=\"filtered\"\u003eApplication\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"font-weight: 400;\"\u003eRNase R serves as a fundamental component in circular RNA synthesis (circRNA). CircRNA holds promise for enhancing pharmaceutical stability and biostability. With their covalently closed structures, circRNAs withstand RNA exonucleases. [1,2] RNase R finds widespread application in various studies, including alternative splicing, intron cDNA production, and the isolation of splicing intermediates and lariats.\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong data-filtered=\"filtered\"\u003eComponents\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable style=\"font-weight: 400;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eComponents No.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eName\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e14615ES25\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e14615ES72\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e14615ES80\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e14615\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eRNase R (GMP-grade, 20 U\/μL)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e25 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e250 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e1 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"font-weight: 400;\"\u003eThis product should be stored at -25~-15℃ for 2 years.\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003eInstructions\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"font-weight: 400;\"\u003ePrepare the following reaction system in a sterile microcentrifuge tube\u003c\/p\u003e\n\u003ctable style=\"font-weight: 400;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003ecomponent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eVolume\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e10×RNase R Reaction Buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e2\u003cspan\u003e \u003c\/span\u003eμL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eRNA sample\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e1\u003cspan\u003e \u003c\/span\u003eμg\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eRNase R (20 U\/μL)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e2-4 U\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eRNase-free ddH\u003csub\u003e2\u003c\/sub\u003eO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eUp to 20\u003cspan\u003e \u003c\/span\u003eμL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp style=\"font-weight: 400;\"\u003eIncubate at 37 ℃ for 10 - 30 minutes.\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\n\u003cstrong\u003e\u003cspan\u003eFigures\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/14615-1_600x600.jpg?v=1744210829\" alt=\"\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: center;\"\u003eFigure 1: Total mRNA (C) can be digested by Yeasen RNAse R. Total mRNA input: 500ng. \u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg style=\"float: none;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/14615-2_600x600.jpg?v=1744210830\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: center;\"\u003eFigure 2: CircRNA can not be digested by Yeasen RNAse R. CircRNA input: 500ng, RNAse R: 4U. \u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/14615-3_1024x1024.jpg?v=1744210830\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: center;\"\u003eFigure 3: Performence of Yeasen RNAse R  (1U to 8U ) digestion on 1 μg of linear mRNA (C).\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/h3\u003e\n\u003col\u003e\n\u003cli style=\"font-weight: 400;\"\u003eThe activity of RNase R requires 0.1-1.0 mM Mg\u003csup\u003e2+\u003c\/sup\u003e;\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003eWith the increase of RNA, the reaction time can be extended and the enzyme amount can be increased appropriately;\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003eThe EDTA content in RNA samples may affect the activity of RNase R.\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003eIncubate at 70 ℃ for 10 minutes can inactivate the enzyme.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003e Reference\u003c\/strong\u003e\u003c\/h3\u003e\n\u003col\u003e\n\u003cli style=\"font-weight: 400;\"\u003eQu L, Yi Z, Shen Y, et al. Circular RNA vaccines against SARS-CoV-2 and emerging variants. Cell. 2022;185(10):1728-1744.e16. doi:10.1016\/j.cell.2022.03.044\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003eChen L, Wang C, Sun H, et al. The bioinformatics toolbox for circRNA discovery and analysis. Brief Bioinform. 2021;22(2):1706-1728. doi:10.1093\/bib\/bbaa001\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\n\u003cstrong\u003eDocuments\u003c\/strong\u003e\u003ca data-v-98c835fc=\"\" href=\"https:\/\/seas.ysbuy.com\/prod\/doc\/mainDoc\/14606ES-RNase%20R%20(20%20U%CE%BCL)-HB230531.pdf\" download=\"\" title=\"download\" class=\"down\" target=\"_blank\"\u003e\u003cimg data-v-98c835fc=\"\" alt=\"\"\u003e \u003c\/a\u003e\n\u003c\/h3\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/14615ES_RNase_R_GMP-grade_20_U_L_-HB240409.pdf?v=1716268060\"\u003eRNAse R GMP-Grade manual\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca data-v-98c835fc=\"\" href=\"https:\/\/seas.ysbuy.com\/prod\/doc\/mainDoc\/14606ES-RNase%20R%20(20%20U%CE%BCL)-HB230531.pdf\" download=\"\" title=\"download\" class=\"down\" target=\"_blank\"\u003e \u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Yeasen","offers":[{"title":"500 U","offer_id":46327976296766,"sku":"14615ES25","price":165.0,"currency_code":"USD","in_stock":true},{"title":"5000U","offer_id":46327976329534,"sku":"14615ES72","price":1365.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/14615.png?v=1766970166"},{"product_id":"10661","title":"Bsa I GMP-grade (20 U\/μL) _ 10661ES","description":"\u003cp\u003eThis product is a type IIS restriction endonuclease derived from the recombinant protein encoded by the BsaI gene in Bacillus sphaericus expressed by E.coli. Its recognition sequence is 5'-GGTCTCN1\/N5-3'. Use to digest plasmids to prepare poly(A\/T\/G\/C)-terminated linearized DNA fragments to obtain specific cohesive ends.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eThis product is produced in accordance with GMP process requirements and provided in a liquid form.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eFeatures\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"p\"\u003e\u003c!-- [if !supportLists]--\u003e1. \u003c!--[endif]--\u003eComplete FDA DMF certification (MF038306)\u003c\/p\u003e\n\u003cp\u003e\u003c!-- [if !supportLists]--\u003e2. \u003c!--[endif]--\u003eGMP-grade, no animal-derived ingredients, strict quality control and production in strict accordance with GMP standards to ensure the introduction of no animal-derived ingredients.\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003c!-- [if !supportLists]--\u003e3. \u003c!--[endif]--\u003eHigh enzyme cutting efficiency, low star activity, and good lethality. It has high enzyme cutting efficiency and no star activity after 16 hours of reaction at 37°C. It has good lethality after enzyme digestion-ligation-re-digestion test.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e4. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eSuperior performance application performance is comparable to competing products\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eApplications\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003ePlasmid linearization for mRNA vaccine production,\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eLentivirus\/adenovirus packaging vector construction,\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eEnzyme digestion verification of plasmid preparation,\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eGolden Gate assembly\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eDNA labeling\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003eSpecifications\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"34.9000%\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eExpression Host\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"65.0800%\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eRecombinant \u003c\/span\u003e\u003ci\u003e\u003cspan\u003eE. coli\u003c\/span\u003e\u003c\/i\u003e\u003cspan\u003e with Bas I gene\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"34.9000%\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eReaction Temperature\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"65.0800%\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e37℃ \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"34.9000%\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eStorage Buffer\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"65.0800%\" valign=\"top\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e10mM Tris-HCl, 0.2M NaCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.2mg\/ml OsrHSA pH 7.4±0.2 (25℃)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"34.9000%\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eUnit Definition\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"65.0800%\" valign=\"top\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e1 unit: The amount of enzyme required to digest 1 μg of substrate DNA within 1 h at 37℃ in a 50 μL system.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"34.9000%\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"65.0800%\" valign=\"top\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e1.Digest the plasmid to prepare a linearized DNA fragment at the end of Poly (A\/T\/C\/G);\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e2.Digestion of DNA to obtain specific sticky ends\u003cspan style=\"font-family: 宋体;\"\u003e；\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e3.Linearize plasmid template before in-vitro transcription.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cbr\u003e\n\u003c\/h3\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"104\" valign=\"top\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"182\" valign=\"top\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"93\" valign=\"top\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e10661ES03\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e(1 KU)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"93\" valign=\"top\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e10661ES10\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e(10 KU)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"93\" valign=\"top\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e10661ES60\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e(100 KU)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"104\" valign=\"top\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e10661\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"182\" valign=\"center\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eBsa I GMP-grade (20 U\/μL)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"93\" valign=\"center\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e50 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"93\" valign=\"center\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e500 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"93\" valign=\"center\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e5 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eShipping and Storage\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThis product should be stored at -25 ~ -15℃ for two years.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eFigures\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" alt=\"Figure1. No non-specific nuclease residues\u0026amp;nbsp;test\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10661_1_1_1024x1024.png?v=1748448075\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: center;\" class=\"p\"\u003e\u003cspan class=\"15\"\u003eFigure1. No non-specific nuclease residues\u003c\/span\u003e\u003cspan class=\"15\"\u003e \u003cspan style=\"font-family: Calibri;\"\u003etest \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e20 U BsaI was incubated with substrate DNA at 37°C for 1 h and 16 h, and DNA band changes were compared by agarose gel electrophoresis. The results showed that there was no non-specific nuclease residue in BsaI.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10661-2_1_1_1024x1024.png?v=1748448436\" alt=\"Figure2. The end integrity is good and the effect is consistent with imported brands\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: center;\" class=\"p\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"15\"\u003eFigure\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Calibri;\"\u003e2\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"15\"\u003e. The end integrity is good and the effect is consistent with imported brands\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eWhen BsaI and substrate DNA were incubated in the enzyme digestion reaction system for 16 hours at 37°C, no non-specific degradation of the substrate caused by star activity was detected, indicating that there was no star activity after 16 hours of incubation with BsaI.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"p\"\u003e\n\u003cb\u003e\u003cspan\u003eDocuments:\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSafety Data Sheet\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/EN-10661-MSDS-HB240611.pdf?v=1776134388\"\u003e\u003cspan\u003e10661_MSDS_HB240611_EN.PDF\u003c\/span\u003e\u003c\/a\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eManuals\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10661ES-BsaI_GMP-grade_20_U_L_-Ver.CN20241220_1.pdf?v=1737603493\"\u003e\u003cspan\u003e10661_Manual_HB20241220_EN.PDF\u003c\/span\u003e\u003c\/a\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Yeasen","offers":[{"title":"500 U","offer_id":46327995990334,"sku":"10661ES03","price":175.0,"currency_code":"USD","in_stock":true},{"title":"10 KU","offer_id":46327996023102,"sku":"10661ES10","price":1210.0,"currency_code":"USD","in_stock":true},{"title":"100 KU","offer_id":51776889815358,"sku":"10661ES60","price":6040.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/products\/10661.jpg?v=1691550803"},{"product_id":"10672","title":"Pyrophosphatase, Inorganic GMP-grade (0.1 U\/μL) -10672ES","description":"\u003cdiv data-v-444b0c7e=\"\" class=\"top_tabc proDetail\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp\u003e\u003cspan\u003eThis product is an inorganic pyrophosphatase derived from recombinant expression in E. coli. It is an enzyme that can catalyze the conversion of one molecule of pyrophosphate into two molecules of phosphate ions. This is a high-energy reaction, so this reaction can be coupled to some thermodynamically unfavorable transformations to drive these transformations to completion. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate. In molecular biology, it can be used to increase RNA yield in reverse transcription reactions.\u003cbr\u003eThis product is produced by GMP process requirements, and the product is provided in liquid form\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003eProduct Properties\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eSource\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eRecombinant E.coli with Pyrophosphatase gene from yeast\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eOptimum Temperature\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e25℃\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eStorage Buffer\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e50 mM Tris-HCl pH 7.9, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% （v\/v）TritonX-100, 50%（v\/v）glycerin\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eUnit Definition\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eOne unit (U) is the amount of enzyme needed to catalyze the hydrolysis of PPI per minute to produce 1 μmol Pi.（The standard reaction is: 500 μL system contains 100 mM Tris-HCl pH7.2, 2 mM MgCl2, 2 mM Ppi; reaction at 25℃ for 10 min）\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003eContents\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\"\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eCatalog No.\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"2\"\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eName\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"3\"\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eCatalog No.\/Specification\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003e10672ES03\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003e(1 U)\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003e10672ES10\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003e(10 U)\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003e10672ES60\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003e(100 U)\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e10672\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003ePyrophosphatase, Inorganic GMP-grade (0.1 U\/μL)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e10 μL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e100 μL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003eShipping and Storage\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003eThe Pyrophosphatase, Inorganic GMP-grade products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003e1. This product is active in a variety of reaction buffers. Usually, this product can be directly connected in experiments such as IVT.\u003cbr\u003e2. The optimal reaction temperature of this product is 25℃, it is active at 16-37℃, and 65℃ can inactivate the enzyme for 10 minutes.\u003cbr\u003e3. For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-v-444b0c7e=\"\" class=\"top_tabc\"\u003e\n\u003ch3 class=\"p\"\u003e\n\u003cb\u003e\u003cspan\u003eDocuments:\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSafety Data Sheet\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10672-MSDS-HB260302.pdf?v=1772418285\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e10672\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e_MSDS_HB\u003c\/span\u003e\u003cspan\u003e260302\u003c\/span\u003e\u003cspan\u003e_EN.PDF\u003c\/span\u003e\u003c\/a\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eManuals\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10672-Manual-HB221008.pdf?v=1772418279\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e10672\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e_Manual_HB221008.\u003c\/span\u003e\u003cspan\u003epdf\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cbr\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\" data-v-444b0c7e=\"\" class=\"cwrap\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\" class=\"list\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen","offers":[{"title":"10 U","offer_id":46328117952830,"sku":"10672ES10","price":70.0,"currency_code":"USD","in_stock":true},{"title":"100 U","offer_id":46328117985598,"sku":"10672ES60","price":475.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10620.png?v=1691751577"},{"product_id":"10670","title":"10× Transcription Buffer 2 GMP-grade _ 10670ES","description":"\u003cdiv data-v-444b0c7e=\"\" class=\"top_tabc proDetail\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp\u003e\u003cspan\u003eThis product is an optimized transcription buffer and is efficient for in vitro synthesis of RNA. This product is mainly composed of buffer salts, reducing agents, and magnesium ions required for enzyme proteins.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis product is produced by GMP regulations and provided in liquid form.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cspan\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eRNA \u003cem\u003ein vitro\u003c\/em\u003e transcription\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eName\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10670ES03\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10670ES10\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10670ES25\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10670ES76\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003e10670\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10× Transcription Buffer 2 GMP-grade\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e25 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e500 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003eThis product could be stored at -25℃ ~ -15℃ for one year.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eNotes\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003eFor your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this product.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"top_tabc\"\u003e\u003cstrong\u003e\u003cspan data-v-444b0c7e=\"\" class=\"boxTap\"\u003eFAQ\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eQ: The transcription buffer of Yisheng has a Mg2+ concentration of 460 mM, which is higher than that of N*, T*, and other domestic manufacturers. Relevant literature reports that the Mg2+ concentration has a significant impact on the amount of transcriptional by-products, dsRNA. Are there any relevant research data?\u003c\/p\u003e\n\u003cp\u003eA: The concentration of our transcribed Buffer is 10X, and the final concentration in the IVT system is 46 mM, which is higher than that of some other brands. We have some data from a single-factor study on Mg2+ (the details are not convenient to disclose). We have determined this concentration. We also provide a buffer without Mg2+ (10669ES) to facilitate customers to adjust the final concentration of Mg2+ in the transcription system by themselves, thereby regulating the balance between IVT yield and by-products.\u003c\/p\u003e\n\u003cp\u003eQ: Are there any differences between 10670ES and 10627ES?\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eA: The formulas are different. The 10670ES is an upgraded version. We highly recommend using the 10670ES. It has a wider application scope and better performance.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"top_tabc\"\u003e\u003cstrong\u003e\u003cspan data-v-444b0c7e=\"\" id=\"tabCBox1\" class=\"boxTap\"\u003eDocuments:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv data-v-444b0c7e=\"\" class=\"top_tabc\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\" data-v-444b0c7e=\"\" class=\"cwrap\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\" class=\"list\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\" class=\"title\"\u003eManuals\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\u003ca data-v-98c835fc=\"\" href=\"https:\/\/seas.ysbuy.com\/prod\/doc\/mainDoc\/10670_Manual_HB230113_EN.pdf\" download=\"\" title=\"download\" class=\"down\" target=\"_blank\"\u003e\n\u003cp data-v-98c835fc=\"\"\u003e10670_Manual_HB230113_EN.pdf\u003c\/p\u003e\n\u003c\/a\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen","offers":[{"title":"1 mL","offer_id":46328118640958,"sku":"10670ES03","price":25.0,"currency_code":"USD","in_stock":true},{"title":"10 mL","offer_id":46328118673726,"sku":"10670ES10","price":235.0,"currency_code":"USD","in_stock":true},{"title":"25 mL","offer_id":50257903223102,"sku":"10670ES25","price":495.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10670_7ba4ca0c-47cd-4343-a523-2c8d09c22bbc.png?v=1768375879"},{"product_id":"10667","title":"10× Digestion buffer 3 GMP-grade _ 10667ES","description":"\u003cdiv class=\"top_tabc proDetail\" data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp class=\"H3_backgro\"\u003eThis product is an optimized 10×Digestion buffer for different restriction enzymes to ensure optimal (100%) digestion activity. The formulation of the product: 500 mM Tris-HCl, 1 M NaCl, 100 mM MgCl\u003csub\u003e2\u003c\/sub\u003e, 1 mg\/mL OsrHSA, pH 7.9 at 25°C.  This product is produced in accordance with GMP process requirements and provided in a liquid form.\u003cbr\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eBuffer for different restriction enzymes to ensure optimal (100%) digestion activity\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eBspQ1 digestion buffer\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eFormat\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eLiquid\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eConcentration\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10X\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eFormulation\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e500 mM Tris-HCl, 1 M NaCl, 100 mM MgCl\u003csub\u003e2\u003c\/sub\u003e, 1 mg\/mL OsrHSA, pH 7.9 at 25°C\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eName\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10667ES03\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10667ES10\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10667ES50\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003e10667\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10×Digestion buffer 3 GMP-grade\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e50 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eShipping and Storage\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003eThe product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eFigures\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/seas.ysbuy.com\/file\/413390\/4f97-167038091005022481.png\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eFigure1. The performance of YEASEN BspQI is superior.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eIn a 50 μL reaction system, 1 μg of λDNA was treated with the corresponding amount of BspQI (incubate at 50 °C for 60 min and then incubate at 80 °C for 20 min to inactivate BspQI). Then 20 μL of the reaction solution was loaded.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eM:  DNA marker\u003cbr\u003eC: The control group without BspQI treatment\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"top_tabc\"\u003e\u003cstrong\u003eDocuments:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"top_tabc\" data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv class=\"cwrap\" data-v-444b0c7e=\"\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"title\" data-v-98c835fc=\"\"\u003eManuals\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cp data-v-98c835fc=\"\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10667_Manual_HB221110_EN.pdf?v=1780972287\" target=\"_blank\" title=\"download\" rel=\"noopener\"\u003e10667_Manual_HB221110_EN.pdf\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen","offers":[{"title":"1 mL","offer_id":46328119984446,"sku":"10667ES03","price":55.0,"currency_code":"USD","in_stock":true},{"title":"10 mL","offer_id":46328120017214,"sku":"10667ES10","price":205.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":46328120049982,"sku":"10667ES50","price":825.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/Fi0ovE55l-VPAN-Vcrcixt3p18P5.png?v=1768375042"},{"product_id":"10666","title":"10× Capping Buffer GMP-grade _10666ES","description":"\u003cdiv data-v-444b0c7e=\"\" class=\"top_tabc proDetail\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp class=\"H3_backgro\"\u003eThis product is optimized 10× capping buffer and is efficient for mRNA capping reaction and 5'end labeling reaction. The formulation of the product: 0.5 M Tris-HCl, 50 mM KCl, 10 mM MgCl2, 10 mM DTT, pH 8.0 at 25℃\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis product is produced in accordance with GMP regulations and provided in a liquid form.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eCapping mRNA prior to translation assays\/in vitro translation\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eComponents No.\u003c\/td\u003e\n\u003ctd\u003eName\u003c\/td\u003e\n\u003ctd\u003e10666ES03\u003c\/td\u003e\n\u003ctd\u003e10666ES10\u003c\/td\u003e\n\u003ctd\u003e10666ES25\u003c\/td\u003e\n\u003ctd\u003e10666ES76\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10666\u003c\/td\u003e\n\u003ctd\u003e10× Capping Buffer GMP-grade\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003ctd\u003e10 mL\u003c\/td\u003e\n\u003ctd\u003e25 mL\u003c\/td\u003e\n\u003ctd\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eShipping and Storage\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003eThis product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eFigures\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/seas.ysbuy.com\/file\/32963\/9369-167030716904910473.png\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFigure 1. The capping efficiency almost reached to 100% by vaccinia capping system from YEASEN.\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eN\u003cspan\u003eucleic acid mass spectrometry before the cap reaction was shown in Figure 1A. Nucleic acid mass spectrometry after the cap reaction was shown in Figure 1B.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-v-444b0c7e=\"\" class=\"top_tabc\"\u003e\n\u003cspan data-v-444b0c7e=\"\" id=\"tabCBox1\" class=\"boxTap\"\u003eDocuments:\u003c\/span\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\" data-v-444b0c7e=\"\" class=\"cwrap\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\" class=\"list\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\" class=\"list\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\" class=\"title\"\u003eSafety Data Sheet\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cp data-v-98c835fc=\"\"\u003e\u003ca rel=\"noopener\" title=\"download\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/MSDS-10666-10xCapping_Buffer_GMP-grade-HB220701.pdf?v=1780971826\" target=\"_blank\"\u003e10666-MSDS-HB220701.pdf\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\" class=\"list\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\" class=\"title\"\u003eManuals\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cp data-v-98c835fc=\"\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10666_Manual_HB221111_EN.pdf?v=1780971822\" target=\"_blank\" title=\"download\" rel=\"noopener\"\u003e10666_Manual_HB221111_EN.pdf\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen","offers":[{"title":"1 mL","offer_id":46328121000254,"sku":"10666ES03","price":55.0,"currency_code":"USD","in_stock":true},{"title":"10 mL","offer_id":46328121033022,"sku":"10666ES10","price":205.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10666.png?v=1691993679"},{"product_id":"10664","title":"BspQI GMP-grade (10 U\/μL) -10664ES","description":"\u003cdiv data-v-444b0c7e=\"\" class=\"top_tabc proDetail\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv style=\"text-align: center;\" data-v-444b0c7e=\"\"\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cspan\u003eThis product is a type II restriction endonuclease derived from the recombinant protein encoded by the BspQI gene in Bacillus sphaericus expressed by E.coli. Its recognition sequence is 5'-GCTCTTCN1\/N4-3'. Use to digest plasmids to prepare poly (A\/T\/G\/C)-terminated linearized DNA fragments to obtain specific cohesive ends. This product is produced in accordance with GMP process requirements and provided in a liquid form.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 style=\"text-align: left;\"\u003e\u003cstrong\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul style=\"text-align: left;\"\u003e\n\u003cli\u003e\u003cstrong\u003eDA DMF Certified** (MF038306)   \u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGMP Grade, Animal-Origin Free: \u003c\/strong\u003eManufactured under ISO 13485 and GMP standards  \u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Efficiency, Low Star Activity:\u003c\/strong\u003e Full digestion at 37°C in 16 hours, no star activity  \u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eNuclease-Free:\u003c\/strong\u003e Rigorously tested and confirmed free of endonucleases, exonucleases, and RNases to ensure sample integrity and reliable downstream results\u003c\/li\u003e\n\u003cli style=\"font-weight: bold;\"\u003e\u003cstrong\u003ePerformance Matches Leading Brands\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"text-align: left;\"\u003e\u003cstrong\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul style=\"text-align: left;\"\u003e\n\u003cli\u003e\u003cspan\u003eDigest the plasmid to prepare a linearized DNA fragment at the end of Poly (A\/T\/C\/G);\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eLinearize plasmid template before in vitro transcription\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eRestriction digest\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\" style=\"text-align: left;\"\u003e\u003cstrong\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\" style=\"text-align: left;\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eExpression Host\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eRecombinant\u003cem\u003e E. coli \u003c\/em\u003ewith BspQI gene\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eReaction Temperature\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e50℃\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eStorage Buffer\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e20 mM Tris-HCl, 0.5 M KCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 50% Glycerol\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eUnit Definition\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e1 unit: The amount of enzyme required to digest 1 μg of λDNA within 1 h at 50℃ in a 50 μL system\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\" style=\"text-align: left;\"\u003e\u003cstrong\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\" style=\"text-align: left;\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eName\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e10664ES76\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(500 U)\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e10664ES86\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(2,500 U)\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e10664ES92\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(10 KU)\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e10664ES98\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(100 KU)\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003e10664\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eBspQI GMP-grade (10 U\/μL)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e50 μL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e250 μL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\" style=\"text-align: left;\"\u003e\u003cstrong\u003e\u003cspan\u003eShipping and Storage\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cspan\u003eThe product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\" style=\"text-align: left;\"\u003e\u003cstrong\u003e\u003cspan\u003eFigures\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cstrong\u003e\u003cspan\u003e1. Enhanced Restriction Enzyme Digestion Efficiency\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" alt=\"Figure 1. Comparison of Digestion Efficiency at Different Enzyme Amounts (0.125–4 U): 2 U Delivers Optimal Performance\" src=\"https:\/\/seas.ysbuy.com\/file\/407108\/32db-167037936011458381.png\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003eFigure 1. Comparison of Digestion Efficiency at Different Enzyme Amounts (0.125–4 U): 2 U Delivers Optimal Performance\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cspan\u003eIn a 50 μL reaction system, 1 μg of λDNA was treated with the corresponding amount of BspQI (incubate at 50 °C for 60 min and then incubate at 80 °C for 20 min to inactivate BspQI). Then 20 μL of the reaction solution was loaded.\u003cbr\u003eM:  DNA marker\u003cbr\u003eC: The control group without BspQI treatment\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cstrong\u003e2. High-Efficiency Enzymatic Digestion in 0.5–3 Hours\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" alt=\"Figure 2. Digestion Performance Across Incubation Times (0.1–3 h): Consistent Results with NEB.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/Yeasen_Bspq1_1024x1024.jpg?v=1739257645\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"text-align: center;\" data-v-444b0c7e=\"\" class=\"top_tabc\"\u003e\n\u003cspan data-v-444b0c7e=\"\" class=\"boxTap\"\u003eFigure 2. Digestion Performance Across Incubation Times (0.1–3 h): Consistent Results with NEB.\u003c\/span\u003e\u003cspan data-v-444b0c7e=\"\" class=\"boxTap\"\u003e\u003c\/span\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"top_tabc\"\u003e\u003cstrong\u003e\u003cspan data-v-444b0c7e=\"\" class=\"boxTap\"\u003eFAQ\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eQ: The enzyme cleavage sites for template linearization are mostly BspQI and bsaI. Why are these two enzymes chosen? I noticed that they both belong to the IIS type restriction endonucleases. Does this have any specific impact?\u003c\/p\u003e\n\u003cp\u003eA: In the aspect of preparing mRNA in vitro transcription templates, the selection of restriction endonucleases is mainly based on the following factors:\u003c\/p\u003e\n\u003cp\u003eThe cut site should be such that the 5' end protrudes or is flush with the end. The presence of the 3' protruding end will increase the generation of false by-products during in vitro transcription (IVT) from the template body.\u003c\/p\u003e\n\u003cp\u003e② The recognition sequence of the restriction enzyme cleavage site should be as long as possible; the longer the recognition sequence, the less likely it is to appear in the gene fragment.\u003c\/p\u003e\n\u003cp\u003e③ IIS-type endonucleases are the preferred enzymes for linearizing plasmids in vaccine development, as their cleavage sites are outside the recognition sequence, ensuring the integrity of the designed poly(A) tail. After linearizing DNA templates using IIS-type endonucleases, no \"scars\" will remain, and no unnecessary nucleotides will be added during the IVT process.\u003c\/p\u003e\n\u003cp\u003e④ The endonucleases meet the pharmaceutical production grade requirements (GMP, without animal-derived components, etc.)\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eAt present, for most mRNA development projects, especially vaccine projects, the endonucleases used for linearization of plasmids are still BspQI, BsaI, and XbaI. Among them, BspQI is the most frequently used. It is estimated that there is a certain relationship with the previous designed plasmid sequences from abroad. Of course, it is not limited to these enzymes; we have also encountered cases where other enzymes were used for linearization.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"top_tabc\"\u003e\u003cstrong\u003e\u003cspan data-v-444b0c7e=\"\" id=\"tabCBox1\" class=\"boxTap\"\u003eDocuments:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv data-v-444b0c7e=\"\" class=\"top_tabc\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\" data-v-444b0c7e=\"\" class=\"cwrap\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\" class=\"list\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\" class=\"title\"\u003eSafety Data Sheet\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cp data-v-98c835fc=\"\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10664-MSDS-HB250708.pdf?v=1751959200\" title=\"download\" rel=\"noopener\" target=\"_blank\"\u003e10664_MSDS_HB250708.pdf\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\" class=\"list\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\" class=\"title\"\u003eManuals\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cp data-v-98c835fc=\"\"\u003e\u003ca rel=\"noopener\" title=\"download\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10664_Manual_HB221110_EN.pdf?v=1779085001\" target=\"_blank\"\u003e10664_Manual_HB221110_EN.pdf\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen","offers":[{"title":"500 U","offer_id":46328121426238,"sku":"10664ES76","price":77.0,"currency_code":"USD","in_stock":true},{"title":"2500 U","offer_id":46328121491774,"sku":"10664ES86","price":295.0,"currency_code":"USD","in_stock":true},{"title":"10 KU","offer_id":46328121557310,"sku":"10664ES92","price":985.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10664.png?v=1691686459"},{"product_id":"10625","title":"T7 RNA Polymerase GMP-grade (250 U\/μL) -10625ES","description":"\u003cdiv data-v-444b0c7e=\"\" class=\"top_tabc proDetail\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp\u003eThis product is a bacteriophage T7 RNA polymerase derived from recombinant protein expression in Escherichia coli. It catalyzes the 5'→3' synthesis of RNA on double-stranded DNA from its T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') and uses NTPs as substrates. The DNA with double-stranded linear blunt ends or 5'protruding ends can be used as templates for T7 RNA polymerase, so linearized plasmids and PCR products can be used as templates for in vitro synthesis of RNA.\u003c\/p\u003e\n\u003cp\u003eNote: G* is the first base of the RNA transcript.\u003c\/p\u003e\n\u003cp\u003eThis product is produced in accordance with GMP regulations and provided in liquid form.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFeature\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSynthesize long transcripts and short transcripts, RNA can be produced 100-200 μg with 1 μg of DNA template\u003c\/li\u003e\n\u003cli\u003eHigher yield and easy to operate\u003c\/li\u003e\n\u003cli\u003eLower endotoxins\u003c\/li\u003e\n\u003cli\u003eTested for the absence of endonucleases, exonucleases, RNases\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eRadiolabeled RNA probe preparation\u003c\/li\u003e\n\u003cli\u003eRNA generation for studies of RNA structure, processing and catalysis\u003c\/li\u003e\n\u003cli\u003eExpression control via anti-sense RNA\u003c\/li\u003e\n\u003cli\u003emRNA, sgRNA synthesis\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eSource\u003c\/td\u003e\n\u003ctd\u003eExpressed in\u003cem\u003e E. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003ewith a recombinant T7 RNA Polymerase gene \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOptimum Temperature\u003c\/td\u003e\n\u003ctd\u003e37℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage Buffer\u003c\/td\u003e\n\u003ctd\u003e50 mM Tris-HCl, 1 mM EDTA, 10 mM DTT, 100 mM NaCl, 0.1% Triton X-100，50% (v\/v) glycerin，pH7.9 at 25℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eUnit Definition\u003c\/td\u003e\n\u003ctd\u003eThe amount of enzyme required to incorporate 1 nmol of [3H] GMP into the acid-insoluble precipitate within 1 hour at 37°C and pH 8.0 is defined as 1 unit.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2 class=\"H2_backgro\"\u003e\n\u003cspan\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003c\/h2\u003e\n\u003ch2 class=\"H2_backgro\"\u003e\n\u003cspan\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/span\u003eQuality\u003c\/h2\u003e\n\u003ctable width=\"1089\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"239\" style=\"width: 350.354px;\"\u003e\u003cspan\u003eCOA Items\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd width=\"850\" style=\"width: 180.979px;\"\u003e\u003cspan\u003eStandard\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"239\" style=\"width: 350.354px;\"\u003e\u003cspan\u003eActivity\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd width=\"850\" style=\"width: 180.979px;\"\u003e\u003cspan\u003e250-300U\/μL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"239\" style=\"width: 350.354px;\"\u003e\n\u003cspan\u003eProtein\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003econcentration\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd width=\"850\" style=\"width: 180.979px;\"\u003e\u003cspan\u003e≥0.5mg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"239\" style=\"width: 350.354px;\"\u003e\u003cspan\u003ePurity\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd width=\"850\" style=\"width: 180.979px;\"\u003e\u003cspan\u003e≥95%\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"239\" style=\"width: 350.354px;\"\u003e\u003cspan\u003eEndotoxin\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd width=\"850\" style=\"width: 180.979px;\"\u003e\u003cspan\u003e\u0026lt;20 EU\/mg\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"239\" style=\"width: 350.354px;\"\u003e\u003cspan\u003eResidual Protease\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd width=\"850\" style=\"width: 180.979px;\"\u003e\u003cspan\u003eNegative\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"239\" style=\"width: 350.354px;\"\u003e\u003cspan\u003eResidual Exonuclease\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd width=\"850\" style=\"width: 180.979px;\"\u003e\u003cspan\u003eNegative\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"239\" style=\"width: 350.354px;\"\u003e\u003cspan\u003eResidual Nickase\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd width=\"850\" style=\"width: 180.979px;\"\u003e\u003cspan\u003eNegative\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"239\" style=\"width: 350.354px;\"\u003e\u003cspan\u003eResidual RNase\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd width=\"850\" style=\"width: 180.979px;\"\u003e\u003cspan\u003eNegative\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"239\" style=\"width: 350.354px;\"\u003e\n\u003cspan\u003eE.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ecoli host protein\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd width=\"850\" style=\"width: 180.979px;\"\u003e\u003cspan\u003e\u0026lt;50ppm\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"239\" style=\"width: 350.354px;\"\u003e\n\u003cspan\u003eE.coli host\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eDNA\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd width=\"850\" style=\"width: 180.979px;\"\u003e\u003cspan\u003e\u0026lt;10 fg\/U\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"239\" style=\"width: 350.354px;\"\u003e\u003cspan\u003eMycoplasma DNA\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd width=\"850\" style=\"width: 180.979px;\"\u003e\u003cspan\u003eNegative\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 class=\"H2_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h2\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eComponents No.\u003c\/td\u003e\n\u003ctd\u003eName\u003c\/td\u003e\n\u003ctd\u003e10625ES10 (10 KU)\u003c\/td\u003e\n\u003ctd\u003e10625ES60 (100 KU)\u003c\/td\u003e\n\u003ctd\u003e10625ES86 (2,500 KU)\u003c\/td\u003e\n\u003ctd\u003e10625ES99 (100 MU)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10625\u003c\/td\u003e\n\u003ctd\u003eT7 RNA Polymerase GMP-grade (250 U\/μL)\u003c\/td\u003e\n\u003ctd\u003e40 μL\u003c\/td\u003e\n\u003ctd\u003e400 μL\u003c\/td\u003e\n\u003ctd\u003e10 mL\u003c\/td\u003e\n\u003ctd\u003e400 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eShipping and Storage\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003eT7 RNA Polymerase GMP-grade products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eFigures\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/seas.ysbuy.com\/file\/693866\/322e-167030612134953504.png\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eFigure 1. Standard RNA was synthesized in vitro using T7 RNA synthesis kit.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads (Cat#12602). The yield result was analyzed by NanoDrop spectrophotometer as shown in Figure 1.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/seas.ysbuy.com\/file\/98179\/8309-167030624349998928.png\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFigures 2. The transcription demonstration of different lengths RNA by T7 kit respectively in the electrophoretogram (Figures 2A), the capillary electrophoresis diagram (Figures 2B), and the chromatogram (Figures 2C)\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0803\/9419\/1166\/files\/T7_480x480.png?v=1700873194\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFigure 3. Synthesis of capped RNA in vitro.\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads (Cat#12602). The yield result was assayed by NanoDrop spectrophotometer as shown in Figure 3A. The integrity result was analyzed by capillary electrophoresis as shown in Figure 3B.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"top_tabc\"\u003e\u003cstrong\u003e\u003cspan data-v-444b0c7e=\"\" class=\"boxTap\"\u003eFAQ\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eQ: How is the fidelity of the T7 enzyme? Have any related tests been conducted?\u003c\/p\u003e\n\u003cp\u003eA: Our T7 enzyme test has measured the relative fidelity, which is similar to that of Thermo. The mutation rate is approximately four parts per million. Currently, the fidelity of the wild-type T7 is quite similar and can basically meet the needs of customers. There is no plan for developing a higher-fidelity T7 in the current project.\u003c\/p\u003e\n\u003cp\u003eQ: Was the yield measured using T7 polymerase after LiCl purification? Are there data for A260\/280 and A260\/230?\u003c\/p\u003e\n\u003cp\u003eA: The measurement was conducted after LiCl purification. We have recorded the relevant data. The ratio of A260\/280 is approximately within the range of 2.0 - 2.1; using our carboxyl magnetic beads for purification also falls within this range. The specific data can be compiled and shared later.\u003c\/p\u003e\n\u003cp\u003eQ: When using T7 polymerase for transcription, is there any way to reduce the problem of excessive polyA tailing?\u003c\/p\u003e\n\u003cp\u003eA: This situation is an inherent characteristic of the T7 reaction system and cannot be completely avoided. According to relevant literature, the main approaches to controlling the self-extension of mRNA are as follows:\u003c\/p\u003e\n\u003cp\u003eOptimize the upstream sequence of the 3' end of the template;\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e② Control the output of IVT. Under high production levels, the probability of self-expansion will be higher.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv data-v-444b0c7e=\"\" class=\"top_tabc\"\u003e\n\u003cstrong\u003e\u003cspan data-v-444b0c7e=\"\" id=\"tabCBox1\" class=\"boxTap\"\u003eDocuments:\u003c\/span\u003e\u003c\/strong\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\" data-v-444b0c7e=\"\" class=\"cwrap\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\" class=\"list\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\" class=\"list\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\" class=\"title\"\u003eSafety Data Sheet\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cp data-v-98c835fc=\"\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10625-MSDS-HB250708.pdf?v=1751959186\" title=\"download\" rel=\"noopener\" target=\"_blank\"\u003e10625_MSDS_HB250708.pdf\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\" class=\"list\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\" class=\"title\"\u003eManuals\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10625-Manual-Ver.EN20260227.pdf?v=1772184850\"\u003e10625_Manual_Ver.EN20260227\u003c\/a\u003e\u003cbr\u003e\n\u003cp data-v-98c835fc=\"\"\u003e\u003cstrong\u003eCitations \u0026amp; References:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv\u003eStamatakos, C. P., Nguyen, H. T., Shekhani, R., Sidhu, G., Maximuck, W. J., Ellington, A. D., \u0026amp; Marcotte, E. M. (2024). High-throughput optimization of T7 promoter variants for fine-tuning in vitro transcription. \u003cem\u003eBiochemistry, 63\u003c\/em\u003e(18), 2307–2318.\u003ca data-v-98c835fc=\"\" href=\"https:\/\/seas.yeasenbiotech.com\/prod\/doc\/mainDoc\/10625_Manual_HB221111_EN.pdf\" download=\"\" title=\"download\" class=\"down\" target=\"_blank\"\u003e \u003c\/a\u003e\u003ca href=\"https:\/\/doi.org\/10.1021\/acs.biochem.4c00188\" rel=\"noopener noreferrer\" target=\"_blank\"\u003ehttps:\/\/doi.org\/10.1021\/acs.biochem.4c00188\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen","offers":[{"title":"10 KU","offer_id":46328130470206,"sku":"10625ES10","price":140.0,"currency_code":"USD","in_stock":true},{"title":"100 KU","offer_id":46328130502974,"sku":"10625ES60","price":1025.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10625.png?v=1751345345"},{"product_id":"10624","title":"T7 RNA Polymerase GMP-grade (50 U\/μL) -10624ES","description":"\u003cdiv class=\"top_tabc proDetail\" data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp\u003eThis product is a bacteriophage T7 RNA polymerase derived from recombinant protein expression in Escherichia coli. It catalyzes the 5'→3' synthesis of RNA on double-stranded DNA from its T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') and uses NTPs as substrates. The DNA with double-stranded linear blunt ends or 5'protruding ends can be used as templates for T7 RNA polymerase, so linearized plasmids and PCR products can be used as templates for in vitro synthesis of RNA.\u003c\/p\u003e\n\u003cp\u003eNote: G* is the first base of the RNA transcript.\u003c\/p\u003e\n\u003cp\u003eThis product is produced in accordance with GMP regulations and provided in liquid form.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFeature\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSynthesize long transcripts and short transcripts, RNA can be produced 100-200 μg with 1 μg of DNA template\u003c\/li\u003e\n\u003cli\u003eHigher yield and easy to operate\u003c\/li\u003e\n\u003cli\u003eLower endotoxins\u003c\/li\u003e\n\u003cli\u003eTested for the absence of endonucleases, exonucleases, RNases\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eRadiolabeled RNA probe preparation\u003c\/li\u003e\n\u003cli\u003eRNA generation for studies of RNA structure, processing and catalysis\u003c\/li\u003e\n\u003cli\u003eExpression control via anti-sense RNA\u003c\/li\u003e\n\u003cli\u003emRNA, sgRNA synthesis\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eSource\u003c\/td\u003e\n\u003ctd\u003eExpressed in\u003cem\u003e E. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003ewith a recombinant T7 RNA Polymerase gene \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOptimum Temperature\u003c\/td\u003e\n\u003ctd\u003e37℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage Buffer\u003c\/td\u003e\n\u003ctd\u003e50 mM Tris-HCl, 1 mM EDTA, 10 mM DTT, 100 mM NaCl, 0.1% Triton X-100，50% (v\/v) glycerin，pH7.9 at 25℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eUnit Definition\u003c\/td\u003e\n\u003ctd\u003eThe amount of enzyme required to incorporate 1 nmol of [3H] GMP into the acid-insoluble precipitate within 1 hour at 37°C and pH 8.0 is defined as 1 unit.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eComponents No.\u003c\/td\u003e\n\u003ctd\u003eName\u003c\/td\u003e\n\u003ctd\u003e10624ES90 (5,000 U)\u003c\/td\u003e\n\u003ctd\u003e10624ES97 (50,000 U)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10624\u003c\/td\u003e\n\u003ctd\u003eT7 RNA Polymerase GMP-grade (50 U\/μL)\u003c\/td\u003e\n\u003ctd\u003e100 μL\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eShipping and Storage\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003eT7 RNA Polymerase GMP-grade products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eFigures\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/seas.ysbuy.com\/file\/504186\/f1f1-167030486328801899.png\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eFigure 1. Standard RNA was synthesized in vitro using T7 RNA synthesis kit.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe reaction was incubated in a PCR instrument at 37℃ for 2h and then purified by magnetic beads (Cat#12602). The yield result was analyzed by NanoDrop spectrophotometer as shown in Figure 1.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/seas.ysbuy.com\/file\/98179\/1d83-167030495757142622.png\" alt=\"\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFigures 2. The transcription demonstration of different lengths RNA by T7 kit respectively in the electrophoretogram (Figures 2A), the capillary electrophoresis diagram (Figures 2B), and the chromatogram (Figures 2C)\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0803\/9419\/1166\/files\/T7_480x480.png?v=1700873194\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eFigure 3. Synthesis of capped RNA in vitro.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe reaction was incubated in a PCR instrument at 37℃ for 2h and then purified by magnetic beads (Cat#12602). The yield result was assayed by NanoDrop spectrophotometer as shown in Figure 3A. The integrity result was analyzed by capillary electrophoresis as shown in Figure 3B.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"top_tabc\"\u003e\u003cstrong\u003e\u003cspan class=\"boxTap\" data-v-444b0c7e=\"\"\u003eFAQ\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eQ: How is the fidelity of the T7 enzyme? Have any related tests been conducted?\u003c\/p\u003e\n\u003cp\u003eA: Our T7 enzyme test has measured the relative fidelity, which is similar to that of Thermo. The mutation rate is approximately four parts per million. Currently, the fidelity of the wild-type T7 is quite similar and can basically meet the needs of customers. There is no plan for developing a higher-fidelity T7 in the current project.\u003c\/p\u003e\n\u003cp\u003eQ: Was the yield measured using T7 polymerase after LiCl purification? Are there data for A260\/280 and A260\/230?\u003c\/p\u003e\n\u003cp\u003eA: The measurement was conducted after LiCl purification. We have recorded the relevant data. The ratio of A260\/280 is approximately within the range of 2.0 - 2.1; using our carboxyl magnetic beads for purification also falls within this range. The specific data can be compiled and shared later.\u003c\/p\u003e\n\u003cp\u003eQ: When using T7 polymerase for transcription, is there any way to reduce the problem of excessive polyA tailing?\u003c\/p\u003e\n\u003cp\u003eA: This situation is an inherent characteristic of the T7 reaction system and cannot be completely avoided. According to relevant literature, the main approaches to controlling the self-extension of mRNA are as follows:\u003c\/p\u003e\n\u003cp\u003eOptimize the upstream sequence of the 3' end of the template;\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e② Control the output of IVT. Under high production levels, the probability of self-expansion will be higher.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"top_tabc\" data-v-444b0c7e=\"\"\u003e\n\u003cspan class=\"boxTap\" id=\"tabCBox1\" data-v-444b0c7e=\"\"\u003eDocuments:\u003c\/span\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv class=\"cwrap\" data-v-444b0c7e=\"\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"title\" data-v-98c835fc=\"\"\u003eSafety Data Sheet\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003ca class=\"down\" title=\"download\" download=\"\" href=\"https:\/\/seas.yeasenbiotech.com\/prod\/doc\/mainDoc\/MSDS-10624-T7%20RNA%20Polymerase%20GMP-grade(50%20U_%CE%BCL)-HB220701.pdf\" data-v-98c835fc=\"\" target=\"_blank\"\u003e\u003cimg alt=\"\" src=\"data:image\/png;base64,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\" data-v-98c835fc=\"\"\u003e\u003c\/a\u003e\n\u003cp data-v-98c835fc=\"\"\u003e\u003ca rel=\"noopener\" title=\"download\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/MSDS-10624-T7_RNA_Polymerase_GMP-grade_50_U__L_-HB220701.pdf?v=1780643517\" target=\"_blank\"\u003eMSDS_10624_HB220701\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"title\" data-v-98c835fc=\"\"\u003eManuals\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003ca class=\"down\" title=\"download\" download=\"\" href=\"https:\/\/seas.yeasenbiotech.com\/prod\/doc\/mainDoc\/10624_Manual_HB221111_EN.pdf\" data-v-98c835fc=\"\" target=\"_blank\"\u003e\u003cimg alt=\"\" src=\"data:image\/png;base64,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\" data-v-98c835fc=\"\"\u003e\u003c\/a\u003e\n\u003cp data-v-98c835fc=\"\"\u003e\u003ca rel=\"noopener\" title=\"download\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10624_Manual_HB221111_EN.pdf?v=1780643887\" target=\"_blank\"\u003e10624_Manual_HB221111\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch3\u003e\u003cstrong\u003eRelated blog：\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp class=\"Polaris-Text--root Polaris-Text--headingLg Polaris-Text--semibold Polaris-Text--base\" tabindex=\"-1\"\u003e\u003ca href=\"\/ar\/blogs\/enzyme\/dnase-i-and-their-applications\"\u003e\u003cstrong\u003eDNase I Applications\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Yeasen","offers":[{"title":"5000 U","offer_id":46328131191102,"sku":"10624ES90","price":70.0,"currency_code":"USD","in_stock":true},{"title":"50000 U","offer_id":46328131223870,"sku":"10624ES97","price":565.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10624.jpg?v=1691687248"},{"product_id":"10623","title":"T7 High Yield RNA Synthesis Kit -10623ES","description":"\u003cdiv class=\"top_tabc proDetail\" data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp\u003eT7 High Yield RNA Synthesis Kit optimizes the transcription reaction system. The kit can synthesize the single-stranded RNA efficiently by using T7 RNA polymerase, the linear double-stranded DNA with the T7 promoter sequence as the template, NTPs as the substrate to control the DNA sequence downstream of the promoter. During transcription, modified nucleotides can be added to the substrate to prepare biotin or dye-labeled RNA.\u003c\/p\u003e\n\u003cp\u003eThis kit can synthesize long transcripts and short transcripts, RNA can be produced 100-200 μg with 1 μg of DNA template input. The RNA synthesized by transcription can be used for various downstream applications, such as RNA structure and function research, RNase protection, probe hybridization, RNAi, microinjection, and in vitro translation.\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/seas.ysbuy.com\/file\/6794\/8909-167029822101711082.png\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFigure 1: In vitro RNA transcription process\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFeature\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eUp to 180 μg of RNA per reaction from 1 μg of the control template\u003c\/li\u003e\n\u003cli\u003eOptimized reaction system for the IVT process\u003c\/li\u003e\n\u003cli\u003eDecrease the dsRNA production\u003c\/li\u003e\n\u003cli\u003eHigher RNA integrity and purity\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eIn vitro RNA synthesis\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eComponents No.\u003c\/td\u003e\n\u003ctd\u003eName\u003c\/td\u003e\n\u003ctd\u003e10623ES50 (50 T)\u003c\/td\u003e\n\u003ctd\u003e10623ES60 (100 T)\u003c\/td\u003e\n\u003ctd\u003e10623ES70 (500 T)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10623-A\u003c\/td\u003e\n\u003ctd\u003eT7 RNA Polymerase Mix\u003c\/td\u003e\n\u003ctd\u003e100 μL\u003c\/td\u003e\n\u003ctd\u003e200 μL\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10623-B\u003c\/td\u003e\n\u003ctd\u003e10×Transcription Buffer\u003c\/td\u003e\n\u003ctd\u003e100 μL\u003c\/td\u003e\n\u003ctd\u003e200 μL\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10623-C\u003c\/td\u003e\n\u003ctd\u003eATP (100mM)\u003c\/td\u003e\n\u003ctd\u003e100 μL\u003c\/td\u003e\n\u003ctd\u003e200 μL\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10623-D\u003c\/td\u003e\n\u003ctd\u003eCTP (100mM)\u003c\/td\u003e\n\u003ctd\u003e100 μL\u003c\/td\u003e\n\u003ctd\u003e200 μL\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10623-E\u003c\/td\u003e\n\u003ctd\u003eGTP (100mM)\u003c\/td\u003e\n\u003ctd\u003e100 μL\u003c\/td\u003e\n\u003ctd\u003e200 μL\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10623-F\u003c\/td\u003e\n\u003ctd\u003eUTP (100mM)\u003c\/td\u003e\n\u003ctd\u003e100 μL\u003c\/td\u003e\n\u003ctd\u003e200 μL\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10623-G\u003c\/td\u003e\n\u003ctd\u003eControl DNA Template (500ng\/μL)\u003c\/td\u003e\n\u003ctd\u003e10 μL\u003c\/td\u003e\n\u003ctd\u003e20 μL\u003c\/td\u003e\n\u003ctd\u003e100 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eShipping and Storage\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003eDry ice transportation. Store at -15℃ ~ -25℃, valid for two years.\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eFigures\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/seas.ysbuy.com\/file\/442040\/54b7-167029833660496583.png\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eFigure 1. Standard RNA was synthesized in vitro using T7 RNA synthesis kit.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe reaction was incubated in a PCR instrument at 37℃ for 2h and then purified by magnetic beads (Cat#12602). The yield result was analyzed by NanoDrop spectrophotometer as shown in Figure 1.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/seas.ysbuy.com\/file\/98179\/18d6-167029881244617985.png\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eFigures 2. The transcription demonstration of different lengths of RNA by T7 kit respectively in the electrophoretogram (Figures 2A), the capillary electrophoresis diagram (Figures 2B), and the chromatogram (Figures 2C)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0803\/9419\/1166\/files\/T7_480x480.png?v=1700873194\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eFigure 3. Synthesis of capped RNA in vitro.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads (Cat#12602). The yield result was assayed by NanoDrop spectrophotometer as shown in Figure 3A. The integrity result was analyzed by capillary electrophoresis as shown in Figure 3B.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"top_tabc\" data-v-444b0c7e=\"\"\u003e\n\u003cspan class=\"boxTap\" id=\"tabCBox1\" data-v-444b0c7e=\"\"\u003eDocuments:\u003c\/span\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv class=\"cwrap\" data-v-444b0c7e=\"\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/MSDS-10623-B-10x_Transcription_Buffer-HB221014.pdf?v=1740624835\"\u003e10623-MSDS-HB221014.pdf\u003c\/a\u003e\u003c\/div\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"title\" data-v-98c835fc=\"\"\u003eManuals\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cp data-v-98c835fc=\"\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10623_Manual_HB230115_EN.pdf?v=1740621738\"\u003e10623_Manual_HB230115\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"top_tabc\" data-v-444b0c7e=\"\"\u003e\n\u003cspan class=\"boxTap\" id=\"tabCBox2\" data-v-444b0c7e=\"\"\u003eFAQs:\u003c\/span\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e1. Low transcript yield\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eThe quality of the template is closely related to the yield. If the yield of the experimental group is significantly lower than the control group, the possible reasons are: \u003c\/p\u003e\n\u003cp\u003e① the experimental template contains inhibitory components; \u003c\/p\u003e\n\u003cp\u003e② The template has something wrong.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSuggestions: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e① Re-purify the template; \u003c\/p\u003e\n\u003cp\u003e② Determine the template quantification and its integrity; \u003c\/p\u003e\n\u003cp\u003e③ Extend the reaction time; \u003c\/p\u003e\n\u003cp\u003e④ Increase the amount of template input; \u003c\/p\u003e\n\u003cp\u003e⑤ Try other promoters and RNA polymerases.\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e2. Low yield of short transcripts\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eA short transcription initiation fragment will inhibit the reaction. When the transcription product is less than 100 nt, extending the reaction time to 4-8 hs or increasing the amount of template to 2 μg will increase RNA yield.\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e3. RNA transcription length is greater than expected\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eIf the electrophoresis shows that the product band is larger than the expected size, the possible reasons: \u003c\/p\u003e\n\u003cp\u003e①The plasmid template may not be completely linearized; \u003c\/p\u003e\n\u003cp\u003e②The 3' end of the sense strand has a prominent structure; \u003c\/p\u003e\n\u003cp\u003e③The RNA has a secondary structure that is not completely denatured.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSuggestions: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e①Check whether the template is completely linearized, and if necessary, perform additional linearization; \u003c\/p\u003e\n\u003cp\u003e②Select a suitable restriction enzyme to avoid 3' overhangs, or use Klenow Fragment \/T4 DNA polymerase to complete the transcription before proceeding; \u003c\/p\u003e\n\u003cp\u003e③Use denatured gel to detect RNA products.\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e4. RNA transcription length is less than expected\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eIf the electrophoresis shows that the product band is smaller than the expected size, the possible reasons: \u003c\/p\u003e\n\u003cp\u003e①The template contains a termination sequence similar to T7 RNA polymerase;\u003c\/p\u003e\n\u003cp\u003e②The GC content in the template is high.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSuggestions: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e①Lower the reaction temperature (for example, 30°C). Sometimes lowering the temperature can increase the transcription length, but it will reduce the yield. Or try different RNA polymerases for transcription; \u003c\/p\u003e\n\u003cp\u003e②If the template GC content is high, use 42℃ to transcript, or add SSB to increase the yield and transcription length.\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e5. Electrophoresis tailing of transcription products\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eThere is a tailing phenomenon during electrophoresis. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePossible reasons:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e①Contaminated by RNase during experimental operation; \u003c\/p\u003e\n\u003cp\u003e②Contaminated DNA template by RNase.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSuggestions: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e①Use RNase-free pipette tips and EP tubes, wear disposable latex gloves and masks, and all reagents are prepared with RNase free H2O. \u003c\/p\u003e\n\u003cp\u003e②Re-purify the template DNA.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"top_tabc\" data-v-444b0c7e=\"\"\u003e\n\u003cspan class=\"boxTap\" id=\"tabCBox3\" data-v-444b0c7e=\"\"\u003eCitations \u0026amp; References:\u003c\/span\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp\u003e\u003cspan\u003e[1] Dong Z, Zheng N, Hu C, et al. Nosema bombycis microRNA-like RNA 8 (Nb-milR8) Increases Fungal Pathogenicity by Modulating BmPEX16 Gene Expression in Its Host, Bombyx mori. Microbiol Spectr. 2021;9(2):e0104821. doi:10.1128\/Spectrum.01048-21(IF:7.171)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e[2] Wang X, Tang S, Ye S, et al. Ultrasensitive quantitation of circulating miR-195-5p with triple strand displacement amplification cascade. Talanta. 2022;242:123300. doi:10.1016\/j.talanta.2022.123300(IF:6.057)\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eFAQ\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eQ: What is the length of the Control DNA Template?\u003c\/p\u003e\n\u003cp\u003eA: The size is 1142 base pairs.\u003c\/p\u003e\n\u003cp\u003eQ: Does the T7 kit have a cap structure and polyA?\u003c\/p\u003e\n\u003cp\u003eA: The polyA tail is introduced during the design of the template, while the addition of the cap is done separately.\u003c\/p\u003e\n\u003cp\u003eQ: How can the IVT system be optimized?\u003c\/p\u003e\n\u003cp\u003eA: ① Increase the input ratio of NTP and its analogues; ② Reasonable application of additives; ③ The reaction temperature can be set at 40 degrees.\u003c\/p\u003e\n\u003cp\u003eThe optimization of the IVT system can be achieved by adjusting the template, the amount of T7 enzyme used, and if it is co-transcription with cap addition, the ratio between the cap and NTP can be considered; for enzymatic cap addition, the conditions can be optimized in terms of the dosage of the cap-adding enzyme, 2-O-methyltransferase, and SAM. Regarding the template, apart from the requirement of complete linearization, there is another point, which is sequence design. For example, in the 5'UTR region, pay attention to uAUG and Kozak sequence; for CDS, consider the codon preference, GC3 content, and dinucleotide preference, etc.; 3'UTR: RBP and miRNA binding sites: and another point is the secondary structure of RNA. The stable secondary structure of the 5'UTR pre-sequence will reduce the expression of mRNA; the stable secondary structure of 3'UTR will increase the stability of mRNA and promote the expression of the encoded protein. For the recommended IVT system and more detailed tips, please refer to the mRNA in vitro synthesis experiment manual of Vivian Biotechnology.\u003c\/p\u003e\n\u003cp\u003eQ: When the IVT reaction is scaled up from a 20 ul system to a 10 ml system, the yield decreases, and the yield per unit volume of the product drops by approximately 50%. Have you encountered this situation before? Do you have any suggestions?\u003c\/p\u003e\n\u003cp\u003eA: When scaling up the 20uL system proportionally to 10mL, the yield should be approximately the same. Usually, we directly scale up from 20uL to 50mL, and the yield is generally at the level of 100ug\/20uL. For the 50mL volume, we also use centrifuge tubes and a metal bath for measurement. For the 10mL volume, the amplification effect is generally better. In such cases, it is generally recommended to fine-tune the final concentrations of the enzyme and NTP before trying again.\u003c\/p\u003e\n\u003cp\u003eQ: How much of the mRNA integrity can be achieved at present?\u003c\/p\u003e\n\u003cp\u003eA: Regarding the re-submissions from our other clients, based on the CE results measured using Qsep100, the ideal situation is that the CE integrity can reach 88% to 92%; of course, achieving this level usually requires a series of optimizations.\u003c\/p\u003e\n\u003cp\u003eThe average yield is between 120-220 ug\/ul (the yield is highly dependent on the sequence design). In the co-transcription method, the mRNA yield is 8 g\/L. With process optimization, the yield will increase. Currently, the quality parameters of the available processes include observing the fluorescence value of GFP and measuring the cap addition rate using LC-MS.\u003c\/p\u003e\n\u003cp\u003eQ: During your own testing process, do you use agarose gel electrophoresis to detect the mRNA products?\u003c\/p\u003e\n\u003cp\u003eA: We can also conduct RNA electrophoresis. The main purpose is to briefly assess the purity of the products and check for any obvious aggregates, etc. If we are looking at the size of the RNA, during the electrophoresis process, we usually add a sample of a clearly defined RNA size as a reference.\u003c\/p\u003e\n\u003cp\u003eQ: Will you conduct a test for the remaining Rnase in the DNA template?\u003c\/p\u003e\n\u003cp\u003eA: Currently, the detection of the template mainly includes items such as sequence integrity, endotoxin, and residual host nucleic acids. There is no clear detection method for RNase yet; or you can refer to the detection method for residual RNase in our enzyme products, which is relatively simple and reliable.\u003c\/p\u003e\n\u003cp\u003eQ: What is the mechanism for the generation of dsRNA during the IVT reaction?\u003c\/p\u003e\n\u003cp\u003eA: There are several possible reasons for the generation of ds during the IVT process, mainly:\u003c\/p\u003e\n\u003cp\u003eThe T7 pol enzyme will use the product RNA as a template and continue to extend the RNA at its 3' end. The extended sequence will form complementary pairing with the 3' end sequence of the original RNA molecule, thereby creating a hairpin structure.\u003c\/p\u003e\n\u003cp\u003e② The T7 pol enzyme can use RNA as a template to replicate RNA molecules, and synthesize the complementary strand.\u003c\/p\u003e\n\u003cp\u003e(At present, there is no effective method for removing dsRNA. However, there is a patent report suggesting that a fiber column (sigma) could be tried.)\u003c\/p\u003e\n\u003cp\u003eQ: Is there a feasible method to control the content of dsRNA in the products of in vitro transcription?\u003c\/p\u003e\n\u003cp\u003eA: The suggestions regarding optimizing the IVT system and adjusting the reaction conditions to inhibit dsRNA mainly include the following points:\u003c\/p\u003e\n\u003cp\u003eReduce the concentration of magnesium ions; however, reducing the concentration of magnesium ions will also lead to a decrease in the production of RNA, and a balance point needs to be reached.\u003c\/p\u003e\n\u003cp\u003e② Increase the reaction temperature of IVT to 50-56℃. This temperature can effectively reduce the content of dsRNA in the product; however, the requirements for equipment for high-temperature reactions will vary, and it may also increase the cost.\u003c\/p\u003e\n\u003cp\u003e③ In the IVT reaction system, certain dispersants such as urea and formamide are added; our process research team has also conducted some experiments on this route, which can achieve the effect of inhibiting dsRNA. However, whether it is suitable for process scaling-up still needs further research.\u003c\/p\u003e\n\u003cp\u003eQ: What are the reasons for the formation of precipitates during the IVT reaction process, and how can it be optimized?\u003c\/p\u003e\n\u003cp\u003eA: Internal research has studied this precipitation. It is a polymer composed of magnesium ions, RNA, sodium ions, and magnesium pyrophosphate. There is a coagulation effect, and the aggregates keep growing in size (similar gel-like, flocculent, or particulate precipitations all follow a similar principle). The effects of adding water and adding EDTA are similar, which also supports this statement. Reasons: 1. If the concentration of Mg ions is too high, it will aggregate with pyrophosphate to form magnesium pyrophosphate precipitation. However, it has a coagulation effect, continuously encapsulating nucleic acids to form a gel-like substance, resulting in a decrease in yield. 2. A high RNA production level will also cause precipitation.\u003c\/p\u003e\n\u003cp\u003eThis kind of precipitation occurs by chance and is also closely related to the sequence design.\u003c\/p\u003e\n\u003cp\u003eSolution: (1) Reduce the concentration of magnesium ions, but this will result in a decrease in yield. The amount of T7 enzyme needs to be increased and the reaction time (from 2 hours to 3-4 hours) needs to be extended; (2) The form of NTP (the presence of magnesium ions at a high concentration, such as 46 mM, does not select NTP, but when the magnesium ion concentration decreases, the yield of NTP (Tris salt form) does not change much, and the yield of the triNa form of NTP decreases significantly.\u003c\/p\u003e\n\u003cp\u003eQ: What are the considerations for reactor selection during the process scale-up of mRNA production?\u003c\/p\u003e\n\u003cp\u003eA: You could consider WAVE, Haodao Fu reaction vessels, and Sartorius. The main thing is to find bags that are compatible with the existing IVT system. For the stirring tank, the rotational speed of the propeller also needs to be optimized based on the actual situation, as it is related to the type and size of the blades. It is recommended to consult the equipment manufacturer.\u003c\/p\u003e\n\u003cp\u003eQ: Do you recommend the two-step method or the one-step method? Why are most of them still using the two-step method at present?\u003c\/p\u003e\n\u003cp\u003eA: In industry, the most commonly used method for enzymatic cap addition is to treat IVT products with vaccinia virus cap-adding enzyme, which can modify them into Cap 0 mRNA. The Cap 0 structure can be further modified into Cap 1 (m7GpppmN) under the action of 2'O-methyltransferase. With enzymatic cap addition, the cap addition efficiency can reach over 95%. The co-transcription cap addition method is simple to operate, but because GTP competes with the cap dimer, the cap addition rate of this method is a little lower; both methods have their own advantages and disadvantages.\u003c\/p\u003e\n\u003cp\u003eQ: What is the output of IVT in vitro transcription?\u003c\/p\u003e\n\u003cp\u003eA: Currently, our system uses a 20-ul reaction volume. The output of in vitro transcription is 100-200 ug. The internal amplification system with a 10 ml volume can reach 50 mg - 100 mg.\u003c\/p\u003e\n\u003cp\u003eQ: When the reaction time exceeds 2 hours during in vitro transcription, will the completeness and yield decrease? How can the generated mRNA be ensured to be stable?\u003c\/p\u003e\n\u003cp\u003eA: It is recommended that the entire reaction time be between 2 and 3 hours. Our company has verified a maximum reaction time of 3 hours, and our cooperative clients have also verified a reaction time of 4 hours, and the results were normal.\u003c\/p\u003e\n\u003cp\u003eQ: Could you please assist in designing the DNA template sequence for in vitro mRNA transcription (mainly for the application in COVID-19 vaccines)?\u003c\/p\u003e\n\u003cp\u003eA: No related services are provided. Currently, the main reference for the COVID-19 vaccine is still the publicly available vaccine sequences from external sources, namely BioNtech and Moderna. The main original sequences are generally reliable, except for the incomplete polyA tail; the earlier COVID-19 vaccines developed in China are mostly based on the RBD protein sequences. We haven't obtained the specific sequences for this part; basically, these are the core technologies of each research team.\u003c\/p\u003e\n\u003cp\u003eQ: What methods and instruments are used for the detection of the cap rate and tailing effect?\u003c\/p\u003e\n\u003cp\u003eA: The capillary column used for adding the cap is from the Agilent series (6230B TOF), while the tailing column used is from Thermo's QE or Waters' Rda.\u003c\/p\u003e\n\u003cp\u003eQ: Do you use tail-adding enzymes when adding the A tail? How many base pairs do you generally recommend for the connection?\u003c\/p\u003e\n\u003cp\u003eA: This reagent kit does not contain the component for poly A tail. In the template preparation stage, the A tail can be attached to the plasmid, and the length is usually between 20 and 200.\u003c\/p\u003e\n\u003cp\u003eQ: In the product purification process, what is the maximum speed for centrifugation? What is the corresponding rotational speed?\u003c\/p\u003e\n\u003cp\u003eA: Any power rating above 13000 rpm is acceptable.\u003c\/p\u003e\n\u003cp\u003eQ: Why did this B component of the Buffer form a precipitate? How should it be dealt with?\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eA: The precipitate is spermidine. This is a normal phenomenon and it can be dissolved by hand.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eRelated blog：\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp class=\"Polaris-Text--root Polaris-Text--headingLg Polaris-Text--semibold Polaris-Text--base\" tabindex=\"-1\"\u003e\u003ca href=\"\/ar\/blogs\/enzyme\/dnase-i-and-their-applications\"\u003e\u003cstrong\u003eDNase I Applications\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen","offers":[{"title":"10 T","offer_id":46328132305214,"sku":"10623ES10","price":35.0,"currency_code":"USD","in_stock":true},{"title":"50 T","offer_id":46328132370750,"sku":"10623ES50","price":165.0,"currency_code":"USD","in_stock":true},{"title":"100 T","offer_id":46328132403518,"sku":"10623ES60","price":295.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10623_10c316e0-21a8-457a-977d-21d30b683188.png?v=1766969927"},{"product_id":"10621","title":"Murine RNase inhibitor GMP-grade (40 U\/μL) _ 10621ES","description":"\u003cdiv class=\"top_tabc proDetail\" data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp\u003eRNase inhibitor can bind RNase to form a complex, thereby specifically inactivating RNase. This product is a recombinant mouse-derived RNase inhibitor expressed and purified in E. coli in a soluble form, which can inhibit various types of RNase (RNase A, B, C). Also tested by RT-PCR and RT-qPCR. Compared with human-derived RNase inhibitor, this product does not contain the two cysteines that are very sensitive to oxidation in human-derived proteins, so it has higher antioxidant activity; and it’s more suitable for high-DTT sensitivity experiments ( such as qPCR, etc.). This product is produced in accordance with GMP process requirements and is provided in liquid form.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eFeature\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eImproved resistance to oxidation, compared to human\/porcine RNase inhibitor\u003c\/li\u003e\n\u003cli\u003eIdeal for reactions where low DTT concentrations are required (e.g., Real-time PCR)\u003c\/li\u003e\n\u003cli\u003eIsolated from a recombinant source\u003c\/li\u003e\n\u003cli\u003eTested for the absence of DNases and RNases\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eRT-PCR\u003c\/li\u003e\n\u003cli\u003ecDNA synthesis\u003c\/li\u003e\n\u003cli\u003eIn vitro transcription\/translation\u003c\/li\u003e\n\u003cli\u003eEnzymatic RNA labeling reaction\u003c\/li\u003e\n\u003cli\u003eOther applications where the integrity of RNA is important\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eSource\u003c\/td\u003e\n\u003ctd\u003eRecombinant E.coli with Murine RNase inhibitor gene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage Buffer\u003c\/td\u003e\n\u003ctd\u003e20 mM Hepes KOH，150 mM KCl，8 mM DTT，0.5%（v\/v）NP 40，0.5%（v\/v）Tween20，50%（v\/v）Glycerol，pH 7.5 ±0.2 (25℃)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eUnit Definition\u003c\/td\u003e\n\u003ctd\u003eThe amount of enzyme required to inhibit 50% of the activity of 5 ng RNase A is defined as 1 activity unit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable style=\"width: 99.9672%;\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 16.2651%;\"\u003eComponents No.\u003c\/td\u003e\n\u003ctd style=\"width: 22.2289%;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 15.3614%;\"\u003e10621ES10 (10 KU)\u003c\/td\u003e\n\u003ctd style=\"width: 15.3614%;\"\u003e10621ES20 (20 KU)\u003c\/td\u003e\n\u003ctd style=\"width: 15.7229%;\"\u003e10621ES60 (100 KU)\u003c\/td\u003e\n\u003ctd style=\"width: 15.1807%;\"\u003e10621ES99 (1 MU)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 16.2651%;\"\u003e10621\u003c\/td\u003e\n\u003ctd style=\"width: 22.2289%;\"\u003eMurine RNase inhibitor GMP-grade (40 U\/μL)\u003c\/td\u003e\n\u003ctd style=\"width: 15.3614%;\"\u003e250 μL\u003c\/td\u003e\n\u003ctd style=\"width: 15.3614%;\"\u003e500 μL\u003c\/td\u003e\n\u003ctd style=\"width: 15.7229%;\"\u003e2.5 mL\u003c\/td\u003e\n\u003ctd style=\"width: 15.1807%;\"\u003e25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eShipping and Storage\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003eThe Murine RNase inhibitor GMP-grade products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"top_tabc\"\u003e\u003cstrong\u003e\u003cspan class=\"boxTap\" id=\"tabCBox1\" data-v-444b0c7e=\"\"\u003eDocuments:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"top_tabc\" data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv class=\"cwrap\" data-v-444b0c7e=\"\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"title\" data-v-98c835fc=\"\"\u003eSafety Data Sheet\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cp data-v-98c835fc=\"\"\u003e\u003ca rel=\"noopener\" title=\"download\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10621-MSDS-HB250708.pdf?v=1751959183\" target=\"_blank\"\u003e10621_MSDS_HB250708.pdf\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"title\" data-v-98c835fc=\"\"\u003eManuals\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cp data-v-98c835fc=\"\"\u003e\u003ca title=\"download\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10621_Manual_HB221111_EN.pdf?v=1779084614\" target=\"_blank\"\u003e10621_Manual_HB221111_EN.pdf\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"top_tabc\"\u003e\n\u003cstrong\u003e\u003cspan class=\"boxTap\" id=\"tabCBox3\" data-v-444b0c7e=\"\"\u003eCitations \u0026amp; References:\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cdiv class=\"top_tabc\" data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp\u003e\u003cspan\u003e[1] Shen W, Wang R, Fan Q, Li Y, Cheng Y. Natural polyphenol assisted delivery of single-strand oligonucleotides by cationic polymers. Gene Ther. 2020;27(7-8):383-391. doi:10.1038\/s41434-020-0151-y(IF:4.128)\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv class=\"cwrap\" data-v-444b0c7e=\"\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003ch3\u003e\u003cstrong\u003eRelated blog：\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp class=\"Polaris-Text--root Polaris-Text--headingLg Polaris-Text--semibold Polaris-Text--base\" tabindex=\"-1\"\u003e\u003ca class=\"down\" title=\"download\" download=\"\" href=\"https:\/\/seas.yeasenbiotech.com\/prod\/doc\/mainDoc\/10133_Manual_HB221109_EN.pdf\" data-v-98c835fc=\"\" target=\"_blank\"\u003e\u003c\/a\u003e\u003ca href=\"\/ar\/blogs\/enzyme\/dnase-i-and-their-applications\"\u003e\u003cstrong\u003eDNase I Applications\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen","offers":[{"title":"10 KU","offer_id":46328132567358,"sku":"10621ES10","price":195.0,"currency_code":"USD","in_stock":true},{"title":"20 KU","offer_id":46328132600126,"sku":"10621ES20","price":355.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10621_dab26665-abb5-40fc-a476-c2365c093e92.png?v=1766970317"},{"product_id":"10620","title":"Pyrophosphatase, Inorganic GMP-grade (1 U\/μL) _ 10620ES","description":"\u003cdiv data-v-444b0c7e=\"\" class=\"top_tabc proDetail\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp\u003eThis product is an inorganic pyrophosphatase derived from recombinant expression in Escherichia coli. It is an enzyme that can catalyze the conversion of one molecule of pyrophosphate into two molecules of phosphate ions. This is a high-energy reaction, so this reaction can be coupled to some thermodynamically unfavorable transformations in order to drive these transformations to completion. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate. In molecular biology, it can be used to increase RNA yield in reverse transcription reactions. This product is produced in accordance with GMP process requirements, and the product is provided in liquid form.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eFeature\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eIsolated from a recombinant source\u003c\/li\u003e\n\u003cli\u003eTested for the absence of endonucleases, exonucleases, RNases\u003c\/li\u003e\n\u003cli\u003eIncreasing RNA yield in transcription reaction; enhancing DNA replication\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eDNA sequencing reaction\u003c\/li\u003e\n\u003cli\u003ePCR and single-base extension reaction\u003c\/li\u003e\n\u003cli\u003eIn vitro transcription processes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eSource\u003c\/td\u003e\n\u003ctd\u003eRecombinant E.coli with Pyrophosphatase gene from yeast\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOptimum Temperature\u003c\/td\u003e\n\u003ctd\u003e25℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage Buffer\u003c\/td\u003e\n\u003ctd\u003e50 mM Tris-HCl pH 7.9，100 mM NaCl，10 mM DTT，1 mM EDTA，0.1% （v\/v）TritonX-100，50%（v\/v）glycerin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eUnit Definition\u003c\/td\u003e\n\u003ctd\u003eOne unit (U) is the amount of enzyme needed to catalyze the hydrolysis of PPI per minute to produce 1 μmol Pi. (The standard reaction is: 500 μL system contains 100 mM Tris-HCl pH7.2, 2 mM MgCl2, 2 mM Ppi; reaction at 25℃ for 10 min)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eComponents No.\u003c\/td\u003e\n\u003ctd\u003eName\u003c\/td\u003e\n\u003ctd\u003e10620ES10 (10 U)\u003c\/td\u003e\n\u003ctd\u003e10620ES60 (100 U)\u003c\/td\u003e\n\u003ctd\u003e10620ES80 (1,000 U)\u003c\/td\u003e\n\u003ctd\u003e10620ES99 (40 KU)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10620\u003c\/td\u003e\n\u003ctd\u003ePyrophosphatase, Inorganic GMP-grade (1 U\/μL)\u003c\/td\u003e\n\u003ctd\u003e10 μL\u003c\/td\u003e\n\u003ctd\u003e100 μL\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003ctd\u003e40 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eShipping and Storage\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003eThe Pyrophosphatase, Inorganic GMP-grade products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eFigures\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/seas.ysbuy.com\/file\/451105\/20b4-167029696689454406.png\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eFigures 1. The relationship between RNA yield and IPPA concentration in vitro transcription\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"top_tabc\"\u003e\u003cstrong\u003e\u003cspan data-v-444b0c7e=\"\" id=\"tabCBox1\" class=\"boxTap\"\u003eDocuments:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv data-v-444b0c7e=\"\" class=\"top_tabc\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\" data-v-444b0c7e=\"\" class=\"cwrap\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\" class=\"list\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\" class=\"title\"\u003eSafety Data Sheet\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cp data-v-98c835fc=\"\"\u003e\u003ca rel=\"noopener\" title=\"download\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10620-MSDS-HB250708_31598b76-ae0c-4192-ba08-6427a969e515.pdf?v=1753170773\" target=\"_blank\"\u003e10620_MSDS_HB250708.pdf\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\" class=\"list\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\" class=\"title\"\u003eManuals\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cp data-v-98c835fc=\"\"\u003e\u003ca rel=\"noopener\" title=\"download\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10620-Manual-Ver.EN220701_64729348-276e-4db5-aec2-8ed093f9774a.pdf?v=1753170769\" target=\"_blank\"\u003e10620-Manual-HB220701.pdf\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"top_tabc\"\u003e\u003cstrong\u003e\u003cspan data-v-444b0c7e=\"\" id=\"tabCBox3\" class=\"boxTap\"\u003eCitations \u0026amp; References:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv data-v-444b0c7e=\"\" class=\"top_tabc\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp\u003e\u003cspan\u003e[1] Li Q, You J, Qiao T, Zhong DB, Yu X. Sodium chloride stimulates the biomass and astaxanthin production by Haematococcus pluvialis via a two-stage cultivation strategy. Bioresour Technol. 2022;344(Pt A):126214. doi:10.1016\/j.biortech.2021.126214(IF:9.642)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e[2] Ma Y, Cheng B, Li Y, et al. Protective Effect of Nanoselenium on Renal Oxidative Damage Induced by Mercury in Laying Hens. Biol Trace Elem Res. 2022;200(8):3785-3797. doi:10.1007\/s12011-021-02956-z(IF:3.738)\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\" data-v-444b0c7e=\"\" class=\"cwrap\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\" class=\"list\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003ch3\u003e\u003cstrong\u003eRelated blog：\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp class=\"Polaris-Text--root Polaris-Text--headingLg Polaris-Text--semibold Polaris-Text--base\" tabindex=\"-1\"\u003e\u003ca data-v-98c835fc=\"\" href=\"https:\/\/seas.yeasenbiotech.com\/prod\/doc\/mainDoc\/10133_Manual_HB221109_EN.pdf\" download=\"\" title=\"download\" class=\"down\" target=\"_blank\"\u003e\u003c\/a\u003e\u003ca href=\"\/ar\/blogs\/enzyme\/dnase-i-and-their-applications\"\u003e\u003cstrong\u003eDNase I Applications\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen","offers":[{"title":"10 U","offer_id":46328133255486,"sku":"10620ES10","price":70.0,"currency_code":"USD","in_stock":true},{"title":"100 U","offer_id":46328133288254,"sku":"10620ES60","price":475.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10620_265f6eee-dc81-4718-89a7-4aaabcb1b4e9.png?v=1691751696"},{"product_id":"10614","title":"mRNA Vaccinia Capping Enzyme GMP-grade (10 U\/μL) _ 10614ES","description":"\u003cdiv class=\"top_tabc proDetail\" data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp\u003e\u003cspan\u003eEukaryotes mRNA forms a special structure at the 5'end after transcription, which is the cap structure. The cap structure plays an important role in the stability, transportation, and translation of mRNA. The vaccinia virus capping enzyme is an effective enzyme that can catalyze the formation of the cap structure. It\"s composed of two subunits D1 and D12. It also has RNA triphosphatase activity, guanylate acyltransferase activity, and guanine methyltransferase activity that could connect the 7-methylguanine cap structure (m7Gppp) to the 5'end of the RNA (m7Gppp5'N). Vaccinia virus capping enzyme can cap the RNA in correct direction within one hour when present at a suitable concentration of capping buffer, guanosine triphosphate (GTP), S-adenosylmethionine (SAM), etc.\u003cbr\u003eThis product is produced by GMP process requirements and provided in a liquid form, used for in vivo\/in vitro pre-translation mRNA capping reaction or mRNA 5'end labeling reaction.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cspan\u003e\u003cstrong\u003eFeature\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eIsolated from a recombinant source\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eTested for the absence of endonucleases, exonucleases, RNases\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\u003cspan\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eCapping mRNA before translation assays\/in vitro translation\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eLabeling 5´ end of mRNA\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eSource\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eRecombinant E. coli with vaccinia virus capping enzyme gene\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eOptimum Temperature\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e37℃\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eStorage Buffer\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e20 mM Tris-HCl pH 8.0, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton X-100, 50% glycerin\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eUnit Definition\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e1 unit: The amount of enzyme required to incorporate 10 pmol GTP (α- 32P) into a transcript with 80 nucleotides (80 nt) at 37℃ within 1 hour. \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eName\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10614ES84 (2,000 U)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10614ES92 (10,000 U)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10614ES96 (100,000 U)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10614ES99 (5 MU)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003e10614\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003emRNA Vaccinia Capping Enzyme GMP-grade (10 U\/μL)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e200 μL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e500 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eShipping and Storage\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003emRNA Vaccinia Capping Enzyme GMP-grade products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eFigures\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/seas.ysbuy.com\/file\/759158\/19c5-167031123115462375.png\" style=\"display: block; margin-left: auto; margin-right: auto;\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan\u003e\u003cstrong\u003eFigure 1. The capping efficiency almost reached 100% by the vaccinia capping system from YEASEN. Nucleic acid mass spectrometry before the cap reaction was shown in Figure 1A. Nucleic acid mass spectrometry after the cap reaction was shown in Figure 1B.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"top_tabc\"\u003e\u003cstrong\u003e\u003cspan class=\"boxTap\" id=\"tabCBox1\" data-v-444b0c7e=\"\"\u003eDocuments:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"top_tabc\" data-v-444b0c7e=\"\"\u003eSafety Data Sheet\u003c\/div\u003e\n\u003cdiv class=\"top_tabc\" data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv class=\"cwrap\" data-v-444b0c7e=\"\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cp data-v-98c835fc=\"\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/MSDS-10614-mRNA_Vaccinia_Capping_Enzyme_GMP-grade_10_U__L_-HB220701.pdf?v=1780369947\" title=\"download\" rel=\"noopener\" target=\"_blank\"\u003e10614_MSDS_HB220701\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"title\" data-v-98c835fc=\"\"\u003eManuals\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cp data-v-98c835fc=\"\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10614_Manual_HB221110_EN.pdf?v=1780369936\"\u003e10614_Manual_HB221110\u003c\/a\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eRelated blog：\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp class=\"Polaris-Text--root Polaris-Text--headingLg Polaris-Text--semibold Polaris-Text--base\" tabindex=\"-1\"\u003e\u003ca href=\"\/ar\/blogs\/enzyme\/dnase-i-and-their-applications\"\u003eDNase I Applications\u003c\/a\u003e\u003c\/p\u003e\n\u003ca title=\"download\" download=\"\" href=\"https:\/\/seas.yeasenbiotech.com\/prod\/doc\/mainDoc\/10614_Manual_HB221110_EN.pdf\" data-v-98c835fc=\"\" target=\"_blank\"\u003e\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen","offers":[{"title":"2 KU","offer_id":46328135287102,"sku":"10614ES84","price":365.0,"currency_code":"USD","in_stock":true},{"title":"10 KU","offer_id":46328135319870,"sku":"10614ES92","price":1445.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10615_5b878751-b652-4ec6-9c7f-9f8f0268be9e.png?v=1766971700"},{"product_id":"10612","title":"mRNA Cap 2'-O-Methyltransferase GMP-grade (50 U\/μl) _ 10612ES","description":"\u003cdiv class=\"top_tabc proDetail\" data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp\u003e\u003cspan\u003emRNA Cap 2´-O-Methyltransferase is a recombinant protein derived from vaccinia virus. The enzyme can add a methyl group at the 2´-O position of the first nucleotide of the cap structure at the 5´ end of the RNA. The enzyme uses SAM as a methyl donor to methylate capped RNA to form a Cap1 structure. The Cap1 structure can enhance the translation efficiency of mRNA, so it can improve the expression of mRNA in transfection and microinjection experiments. This enzyme requires RNA with m7GpppN, a 7-methylguanosine cap structure, as a substrate.\u003cbr\u003eThis product is produced in accordance with GMP process requirements. The product is provided in liquid form and can be used for Cap1 capping reaction of pre-mRNA in vivo\/in vitro.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cspan\u003e\u003cstrong\u003eFeature\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eIsolated from a recombinant source\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eTested for the absence of endonucleases, exonucleases, Rnases\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\u003cspan\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003e2´-O-methylation of capped in vitro transcripts helps the RNA evade innate immune response in vivo\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003emRNA cap-1 structure may improve expression\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eSource\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eRecombinant E.coli with Cap 2´-O-Methyltransferase gene\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eOptimum Temperature\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e37℃\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eStorage Buffer\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e20 mM Tris-HCl pH8.0, 0.1mM EDTA, 1mM DTT, 100mM NaCl, 50% (v\/v) glycerin, 0.1% (v\/v) Trion X-100  \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eUnit Definition\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eOne unit is defined as the amount of enzyme required to methylate 10 pmol of 80nt capped RNA transcript in 1 h at 37°C.\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eName\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e10612ES92\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(10,000 U)\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e10612ES97\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(50,000 U)\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e10612ES98\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(250,000 U)\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e10612ES99\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(20 MU)\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003e10612\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003emRNA Cap 2´-O-Methyltransferase GMP-grade (50 U\/μl)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e200 μL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e5 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e400 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eShipping and Storage\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003eThe mRNA Cap 2´-O-Methyltransferase GMP-grade products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eFigures\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/seas.ysbuy.com\/file\/369399\/6593-167030752734177646.png\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003e\u003cspan\u003eFigures 1. The relationship between capping efficiency and 2’-O-Methyltransferase quantity\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"top_tabc\"\u003e\u003cstrong\u003e\u003cspan class=\"boxTap\" id=\"tabCBox1\" data-v-444b0c7e=\"\"\u003eDocuments:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"top_tabc\" data-v-444b0c7e=\"\"\u003eSafety Data Sheet\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv class=\"cwrap\" data-v-444b0c7e=\"\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\u003ca rel=\"noopener\" class=\"down\" title=\"download\" download=\"\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/MSDS-10612-mRNA_Cap_2_-O-Methyltransferase_GMP-grade_50_U__l_-HB220701.pdf?v=1780368585\" data-v-98c835fc=\"\" target=\"_blank\"\u003e\n\u003cp data-v-98c835fc=\"\"\u003e10612_MSDS_HB220701\u003c\/p\u003e\n\u003c\/a\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"title\" data-v-98c835fc=\"\"\u003eManuals\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cp data-v-98c835fc=\"\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10612_Manual_HB221110_EN.pdf?v=1780368561\"\u003e10612_Manual_HB221110\u003c\/a\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eRelated blog：\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp class=\"Polaris-Text--root Polaris-Text--headingLg Polaris-Text--semibold Polaris-Text--base\" tabindex=\"-1\"\u003e\u003ca class=\"down\" title=\"download\" download=\"\" href=\"https:\/\/seas.yeasenbiotech.com\/prod\/doc\/mainDoc\/10612_Manual_HB221110_EN.pdf\" data-v-98c835fc=\"\" target=\"_blank\"\u003e\u003c\/a\u003e\u003ca href=\"\/ar\/blogs\/enzyme\/dnase-i-and-their-applications\"\u003eDNase I Applications\u003c\/a\u003e\u003c\/p\u003e\n\u003cp data-v-98c835fc=\"\"\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen","offers":[{"title":"10000 U","offer_id":46328136630590,"sku":"10612ES92","price":165.0,"currency_code":"USD","in_stock":true},{"title":"50000 U","offer_id":46328136663358,"sku":"10612ES97","price":685.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10612_023fdc5a-12fa-45fc-964e-60e6029be331.png?v=1766971315"},{"product_id":"10611","title":"Deoxyribonuclease I (DNase I) GMP-grade (2 U\/μL) _ 10611ES","description":"\u003cdiv class=\"top_tabc proDetail\" data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv style=\"text-align: start;\" data-v-444b0c7e=\"\"\u003e\n\u003cp\u003e\u003cspan\u003eDNase I is an endonuclease that can digest single-stranded and double-stranded DNA to produce single deoxynucleotides or single-stranded or double-stranded oligodeoxynucleotides. It can hydrolyze the phosphodiester bond to produce monodeoxynucleotides and oligodeoxynucleotides containing 5'-phosphate groups and 3'-OH groups. The average digestion product is the smallest polytetranucleotide. DNase I can catalyze many forms of DNA, such as single-stranded DNA, double-stranded DNA, and even chromatin (its cutting rate is affected by histones). The optimum pH range is 7-8. The activity of DNase I depends on Ca\u003csup\u003e2+\u003c\/sup\u003e and can be activated by divalent metal ions, such as Co\u003csup\u003e2+\u003c\/sup\u003e, Mn\u003csup\u003e2+\u003c\/sup\u003e, Zn\u003csup\u003e2+\u003c\/sup\u003e, etc. 5 mM Ca\u003csup\u003e2+\u003c\/sup\u003e can protect the enzyme from being hydrolyzed. In the presence of Mg\u003csup\u003e2+\u003c\/sup\u003e, the enzyme can recognize and cut any site on any strand of DNA randomly; and in the presence of Mn\u003csup\u003e2+\u003c\/sup\u003e, it can recognize two strands of DNA at the same time and cut at almost the same site to form blunt ends, Or sticky ends with 1-2 nucleotides protruding. DNase I is widely used in the preparation of DNA-free RNA; remove the template DNA after in vitro transcription; prepare DNA-free RNA before RT-PCR and RT-qPCR reactions; combine with DNA polymerase I to perform DNA labeling through nick translocation; DNA fragmentation library construction.\u003cbr\u003eThis product is produced in accordance with GMP process requirements, and the product is provided in liquid form.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cspan\u003e\u003cstrong\u003eFeature\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eDNA specific Endonuclease\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eDegrades double-stranded and single-stranded DNA\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eProducts are short oligos with 5'-phosphate and 3'-OH\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\u003cspan\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eRemoval of contaminating genomic DNA from RNA samples\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eDegradation of DNA templates in transcription reactions\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eSource\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eRecombinant E. coli with DNase I gene\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eOptimum temperature\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e37℃\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eStorage Buffer\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10 mM Tris-HCl pH 7.6，2 mM CaCl\u003csub\u003e2\u003c\/sub\u003e, 50% (v\/v) Glycerol \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eUnit Definition\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eThe amount of enzyme required to completely degrade 1 μg of plasmid DNA within 10 min at 37°C. (The reaction buffer is: 10 mM Tris-HCl pH 7.6, 2.5 mM MgCl\u003csub\u003e2\u003c\/sub\u003e, 0.5 mM CaCl\u003csub\u003e2\u003c\/sub\u003e, 1 μg plasmid DNA)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eName\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e10611ES76\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(500 U)\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e10611ES84\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(2,000 U)\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e10611ES92\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(10,000 U)\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003e10611\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eDeoxyribonuclease I (DNase I) GMP-grade (2 U\/μL)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e250 μL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e5 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eShipping and Storage\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003eDeoxyribonuclease I (DNase I) GMP-grade products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eFigures\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg style=\"margin-bottom: 16px; float: none; display: block; margin-left: auto; margin-right: auto;\" alt=\"Figure 1. DNase I from YEASEN could effectively remove DNA template during transcription，compared with competitor brands.\" src=\"https:\/\/seas.ysbuy.com\/file\/309671\/68ab-167030548627849912.png\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003e\u003cspan\u003eFigure 1. DNase I from YEASEN could effectively remove DNA template during transcription，compared with competitor brands.\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"top_tabc\"\u003e\u003cstrong\u003e\u003cspan class=\"boxTap\" id=\"tabCBox1\" data-v-444b0c7e=\"\"\u003eDocuments:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"top_tabc\" data-v-444b0c7e=\"\"\u003eSafety Data Sheet\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv class=\"cwrap\" data-v-444b0c7e=\"\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cp data-v-98c835fc=\"\"\u003e\u003ca rel=\"noopener\" title=\"download\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/MSDS-10611-Deoxyribonuclease_I_DNase_I_GMP-grade_2_U__L_-HB220701.pdf?v=1780367807\" target=\"_blank\"\u003e10611_MSDS_HB220701\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"title\" data-v-98c835fc=\"\"\u003eManuals\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cp data-v-98c835fc=\"\"\u003e\u003ca rel=\"noopener\" title=\"download\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10611_Manual_HB221110_EN.pdf?v=1780367808\" target=\"_blank\"\u003e10611_Manual_HB221110\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"top_tabc\"\u003e\u003cstrong\u003e\u003cspan class=\"boxTap\" id=\"tabCBox3\" data-v-444b0c7e=\"\"\u003eCitations \u0026amp; References:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"top_tabc\" data-v-444b0c7e=\"\"\u003e\n\u003cspan\u003e[1] Lu Z, Liu H, Song N, et al. METTL14 aggravates podocyte injury and glomerulopathy progression through N\u0026lt;sup\u0026gt;6\u0026lt;\/sup\u0026gt;-methyladenosine-dependent downregulating of Sirt1. Cell Death Dis. 2021;12(10):881. Published 2021 Sep 27. doi:10.1038\/s41419-021-04156-y(IF:8.469)\u003c\/span\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp\u003e\u003cspan\u003e[2] Yu Y, Wang Y, Zhang W, et al. Biomimetic periosteum-bone substitute composed of preosteoblast-derived matrix and hydrogel for large segmental bone defect repair. Acta Biomater. 2020;113:317-327. doi:10.1016\/j.actbio.2020.06.030(IF:7.242)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"top_tabc\" data-v-444b0c7e=\"\"\u003e\u003cspan class=\"boxTap\" id=\"tabCBox4\" data-v-444b0c7e=\"\"\u003eCOA\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv class=\"top_tabc\" data-v-444b0c7e=\"\"\u003e\n\u003cspan class=\"boxTap\" data-v-444b0c7e=\"\"\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"top_tabc\" data-v-444b0c7e=\"\"\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\u003cstrong\u003eRelated blog：\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003ca href=\"\/ar\/blogs\/enzyme\/dnase-i-demystified-how-to-break-through-the-bottleneck-of-traditional-technology-by-expressing-dnase-i-in-yeast\"\u003eDNase I Demystified | How to break through the bottleneck of traditional technology by expressing DNase I in yeast\u003c\/a\u003e\u003c\/p\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv class=\"cwrap\" data-v-444b0c7e=\"\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cp class=\"Polaris-Text--root Polaris-Text--headingLg Polaris-Text--semibold Polaris-Text--base\" tabindex=\"-1\"\u003e\u003ca class=\"down\" title=\"download\" download=\"\" href=\"https:\/\/seas.yeasenbiotech.com\/prod\/doc\/mainDoc\/10133_Manual_HB221109_EN.pdf\" data-v-98c835fc=\"\" target=\"_blank\"\u003e\u003c\/a\u003e\u003ca href=\"\/ar\/blogs\/enzyme\/dnase-i-and-their-applications\"\u003eDNase I Applications\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen","offers":[{"title":"500 U","offer_id":46328136728894,"sku":"10611ES76","price":35.0,"currency_code":"USD","in_stock":true},{"title":"2000 U","offer_id":46328136761662,"sku":"10611ES84","price":105.0,"currency_code":"USD","in_stock":true},{"title":"10000 U","offer_id":46328136794430,"sku":"10611ES92","price":515.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10611.png?v=1691752566"},{"product_id":"10628","title":"Hieff™ T7 RNA polymerase (low dsRNA,250 U\/μL)_ 10628ES","description":"\u003cdiv class=\"top_tabc proDetail\" data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp\u003eThis is an engineered T7 RNA polymerase variant (low dsRNA) derived from the wild-type T7 RNA polymerase and produced in Escherichia coli. It significantly reduces the production of double-stranded RNA (dsRNA) while efficiently incorporating cap analogs, and exhibiting highly efficient in vitro transcription (IVT) comparable to the wild-type T7 RNA polymerase. It catalyzes the 5'→3' synthesis of RNA on double-stranded DNA from its T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') and uses NTPs as substrates.\u003c\/p\u003e\n\u003cp\u003eNote: G* is the first base of the RNA transcript.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #ff2a00;\"\u003e\u003cstrong\u003eThe original brand name of the product is CleaScrip™.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eFeature\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eReduced dsRNA Content:\u003c\/strong\u003e dsRNA content is reduced by more than 10-fold.  \u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Yield:\u003c\/strong\u003e Stable yield of over 9 mg\/mL.  \u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLow Cap Analog Input**:\u003c\/strong\u003e Cap Analog input can be reduced to 2.5 mM.  \u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Capping Efficiency**: \u003c\/strong\u003eCapping efficiency exceeds 99%.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeabio.com\/blogs\/news\/low-dsrna-t7-rna-polymerase\"\u003e\u003cspan\u003ePlease find information on the development of this enzyme.\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003e\u003cb\u003eComponents\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable cellspacing=\"2\" border=\"0\" style=\"width: 101.411%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"170\" style=\"width: 15.6974%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"261\" style=\"width: 22.9876%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"177\" style=\"width: 15.1141%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10628ES10\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"172\" style=\"width: 15.1141%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10628ES60\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"156\" style=\"width: 15.1141%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10628ES72\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"196\" style=\"width: 15.1141%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10628ES86\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"170\" style=\"width: 15.6974%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"261\" style=\"width: 22.9876%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"177\" style=\"width: 15.1141%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e(10 KU)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"172\" style=\"width: 15.1141%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e(100 KU)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"156\" style=\"width: 15.1141%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e(250 KU)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"196\" style=\"width: 15.1141%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e(2,500 KU)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"170\" style=\"width: 15.6974%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10628\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"261\" style=\"width: 22.9876%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003eHieff T7 RNA Polymerase (low dsRNA, 250 U\/μL)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"177\" style=\"width: 15.1141%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e40 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"172\" style=\"width: 15.1141%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e400 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"156\" style=\"width: 15.1141%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"196\" style=\"width: 15.1141%;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cstrong\u003e\u003cb\u003eSpecifications \u003c\/b\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"301\"\u003e\n\u003cp\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"982\"\u003e\n\u003cp\u003eRecombinant\u003cem\u003e\u003ci\u003e \u003c\/i\u003e\u003c\/em\u003e\u003cem\u003e\u003ci\u003eE. coli \u003c\/i\u003e\u003c\/em\u003ewith T7 RNA Polymerase gene\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"301\"\u003e\n\u003cp\u003e\u003cstrong\u003eOptimum Temperature\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"982\"\u003e\n\u003cp\u003e37℃\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"301\"\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"982\"\u003e\n\u003cp\u003e50 mM Tris-HCl, 1 mM EDTA, 10 mM DTT, 100 mM NaCl, 0.1% Triton X-100，50% (v\/v) glycerin，pH7.9 at 25℃\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"301\"\u003e\n\u003cp\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"982\"\u003e\n\u003cp\u003eThe amount of enzyme required to incorporate 1 nmol of [\u003csup\u003e3\u003c\/sup\u003eH] GMP into the acid-insoluble precipitate within 1 hour at 37°C and pH 8.0 is defined as 1 unit.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eRecommended Mg2+\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e30mM Magnesium Acetate\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\n\u003cstrong\u003e\u003cb\u003eQC \u003cspan\u003eStandard\u003c\/span\u003e\u003c\/b\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/strong\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"425\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003e\u003cspan style=\"font-family: Calibri;\"\u003eI\u003c\/span\u003etems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"731\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eSpecification\/Standard\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"425\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eEnzyme\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eactivity\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"731\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e250\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003e-\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e300U\/μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"425\" valign=\"top\"\u003e\n\u003cp\u003eProtein Purity\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"731\" valign=\"top\"\u003e\n\u003cp\u003e≥95%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"425\" valign=\"top\"\u003e\n\u003cp\u003eEndotoxin\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"731\" valign=\"top\"\u003e\n\u003cp\u003e\u0026lt;20 EU\/mg\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"425\" valign=\"top\"\u003e\n\u003cp\u003eProtease\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"731\" valign=\"top\"\u003e\n\u003cp\u003eNegative\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"425\" valign=\"top\"\u003e\n\u003cp\u003eExonuclease\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"731\" valign=\"top\"\u003e\n\u003cp\u003eNegative\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"425\" valign=\"top\"\u003e\n\u003cp\u003eNickase\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"731\" valign=\"top\"\u003e\n\u003cp\u003eNegative\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"425\" valign=\"top\"\u003e\n\u003cp\u003eRNase\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"731\" valign=\"top\"\u003e\n\u003cp\u003eNegative\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"425\" valign=\"top\"\u003e\n\u003cp\u003eE.coli host protein\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"731\" valign=\"top\"\u003e\n\u003cp\u003e\u0026lt;50ppm\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"425\" valign=\"top\"\u003e\n\u003cp\u003eE.coli host DNA\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"731\" valign=\"top\"\u003e\n\u003cp\u003e\u0026lt;10 fg\/U\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"425\" valign=\"top\"\u003e\n\u003cp\u003eMycoplasma examination\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"731\" valign=\"top\"\u003e\n\u003cp\u003eNegative\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cstrong\u003e\u003cb\u003eRelated Products\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeabio.com\/blogs\/mrna\/gmp-grade-enzymes\"\u003e\u003cspan\u003eTris NTPs synergistically decreasing dsRNA\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeabio.com\/products\/10670\"\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e10× Transcription Buffer 2 GMP-grade 10670ES\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e\u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e\u003c\/span\u003e\u003ca href=\"https:\/\/www.yeabio.com\/products\/10657\"\u003e\u003cspan style=\"font-size: 0.875rem;\" data-v-9e66f18e=\"\"\u003eN1-Me-Pseudo UTP Tris Solution GMP-grade (100 mM) \u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\" data-v-9e66f18e=\"\" class=\"code\"\u003e10657ES\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeabio.com\/products\/10656\"\u003e\u003cspan data-v-9e66f18e=\"\" style=\"font-size: 0.875rem;\"\u003ePseudo UTP Tris Solution GMP-grade (100 mM) \u003c\/span\u003e\u003cspan data-v-9e66f18e=\"\" class=\"code\" style=\"font-size: 0.875rem;\"\u003e10656ES\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeabio.com\/products\/10652\"\u003e\u003cspan data-v-9e66f18e=\"\" style=\"font-size: 0.875rem;\"\u003eATP Tris Solution GMP-grade (100 mM) \u003c\/span\u003e\u003cspan data-v-9e66f18e=\"\" class=\"code\" style=\"font-size: 0.875rem;\"\u003e10652ES\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeabio.com\/products\/10655\"\u003e\u003cspan data-v-9e66f18e=\"\" style=\"font-size: 0.875rem;\"\u003eGTP Tris Solution GMP-grade (100 mM) \u003c\/span\u003e\u003cspan data-v-9e66f18e=\"\" class=\"code\" style=\"font-size: 0.875rem;\"\u003e10655ES\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeabio.com\/products\/10653\"\u003e\u003cspan data-v-9e66f18e=\"\" style=\"font-size: 0.875rem;\"\u003eCTP Tris Solution GMP-grade (100 mM) \u003c\/span\u003e\u003cspan data-v-9e66f18e=\"\" class=\"code\" style=\"font-size: 0.875rem;\"\u003e10653ES\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeabio.com\/products\/10654\"\u003e\u003cspan style=\"font-size: 0.875rem;\" data-v-9e66f18e=\"\"\u003eUTP Tris Solution GMP-grade (100 mM) \u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\" data-v-9e66f18e=\"\" class=\"code\"\u003e10654ES\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeabio.com\/products\/36717\"\u003e\u003cspan data-v-9e66f18e=\"\" style=\"font-size: 0.875rem;\"\u003eDouble-stranded RNA (dsRNA) ELISA kit 36717ES\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003ch3\u003e\u003cstrong\u003e\u003cb\u003eFigures\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e1. 8K Sequence Validation – WT-T7 vs. T7 (Low dsRNA)\u003c\/span\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003ctable border=\"0\" cellpadding=\"0\" cellspacing=\"0\" style=\"width: 537pt; height: 117px; border-collapse: collapse;\" width=\"716\"\u003e\n\u003ctbody\u003e\n\u003ctr height=\"39\" style=\"height: 39px;\"\u003e\n\u003ctd class=\"kso0\" style=\"width: 134pt; height: 39px;\" width=\"179\"\u003e\n\u003cp\u003eT7 RNA Polymerase\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"179\"\u003e\n\u003cp\u003eYield(mg\/mL)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"179\"\u003e\n\u003cp\u003emRNA Integrity(%)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd class=\"kso3\" style=\"width: 134pt; height: 39px;\" width=\"179\"\u003e\n\u003cp\u003e dsRNA content (%)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr height=\"39\" style=\"height: 39px;\"\u003e\n\u003ctd class=\"kso4\" style=\"width: 134pt; height: 39px;\" width=\"179\"\u003e\n\u003cp\u003eWT T7\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"179\"\u003e\n\u003cp\u003e9.5\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"179\"\u003e\n\u003cp\u003e81.9\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd class=\"kso7\" style=\"width: 134pt; height: 39px;\" width=\"179\"\u003e\n\u003cp\u003e0.29%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr height=\"39\" style=\"height: 39px;\"\u003e\n\u003ctd class=\"kso8\" style=\"width: 134pt; height: 39px;\" width=\"179\"\u003e\n\u003cp\u003eLow dsRNA T7\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"179\"\u003e\n\u003cp\u003e9.0\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"179\"\u003e\n\u003cp\u003e82\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd class=\"kso11\" style=\"width: 134pt; height: 39px;\" width=\"179\"\u003e\n\u003cp\u003e0.0037%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv data-v-444b0c7e=\"\" style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/2_1024x1024_0c1a6975-608a-4816-a261-f01b84533985_1024x1024.png?v=1756175810\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e2. Capping Efficiency \u0026amp; Cellular Function Validation:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv data-v-444b0c7e=\"\" style=\"text-align: center;\"\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/3_1de49755-501c-43dc-bef9-cdeef6f424c0_1024x1024.png?v=1755483556\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eFor both 4K and 1K fragments, capping efficiency was evaluated at different cap analog input amounts, showing that the input can be reduced to as low as 2.5 mM without significant differences in capping efficiency or cellular function at 48 h. No significant differences were observed between T7 mutant and wild-type (WT) enzymes.\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eCustomer feedback:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv data-v-444b0c7e=\"\" style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/dsRNA_figure_68789415-4b42-4894-84b5-a93b08e8d406_1024x1024.jpg?v=1714285432\" alt=\"Figure 1. Testing of T7 RNA Polymerase mutants. dsRNA Content: The dsRNA content reduced by more than 10-fold (A).\u0026amp;nbsp; mRNA Integrity: Maintained at over 90% (B).\u0026amp;nbsp; 2K Fragment Capping Efficiency: Exceeded 99% (C, D). High integrity (E)\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003eE\u003c\/div\u003e\n\u003cdiv data-v-444b0c7e=\"\" style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10625-1_1024x1024.jpg?v=1749787317\" alt=\"Figure 1. Testing of T7 RNA Polymerase mutants. dsRNA Content: The dsRNA content reduced by more than 10-fold (A).\u0026amp;nbsp; mRNA Integrity: Maintained at over 90% (B).\u0026amp;nbsp; 2K Fragment Capping Efficiency: Exceeded 99% (C, D). High integrity (E)\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cdiv data-v-444b0c7e=\"\" style=\"text-align: center;\"\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eFigure 1. Testing of T7 RNA Polymerase mutants. dsRNA Content: The dsRNA content reduced by more than 10-fold (A).  mRNA Integrity: Maintained at over 90% (B).  2K Fragment Capping Efficiency: Exceeded 99% (C, D). High integrity (E)\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cspan style=\"font-size: 0.875rem;\"\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003cdiv data-v-444b0c7e=\"\" style=\"text-align: center;\"\u003e\n\u003cdiv data-v-9e66f18e=\"\" class=\"ListTitle\"\u003e\n\u003cdiv data-v-9e66f18e=\"\"\u003e\n\u003cdiv data-v-9e66f18e=\"\" class=\"ListTitle\"\u003e\n\u003cspan data-v-9e66f18e=\"\" class=\"code\"\u003e\u003cspan data-v-9e66f18e=\"\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/Yields.jpg?v=1714284938\" alt=\"\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003ca data-v-9e66f18e=\"\" href=\"https:\/\/www.yeasenbiotech.com\/productdetail\/2858\"\u003e\u003cbr\u003e\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv data-v-9e66f18e=\"\" class=\"ListTitle\"\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eTable 1. The Yields of low dsRNA T7 RNA polymerase is comparable with that of WT.\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv data-v-9e66f18e=\"\" class=\"ListTitle\"\u003e\n\u003cspan style=\"font-size: 0.875rem;\"\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/t61_1024x1024.png?v=1717802537\" alt=\"Figure 2. Evaluation of the immunogenicity of the IVT products in murine RAW264.7 cells (Figures 2A and 2B). IFN-β mRNA and protein levels were reduced in RAW264.7 cells transfected with mRNA produced by mutants compared to wild-type, indicating that mRNA synthesized by the wild-type T7 RNAP elicited the strongest immune response, while mRNA from the mutants showed a significantly reduced response.\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/h2\u003e\n\u003cp style=\"text-align: center;\"\u003eFigure 2. Evaluation of the immunogenicity of the IVT products in murine RAW264.7 cells (Figures 2A and 2B). IFN-β mRNA and protein levels were reduced in RAW264.7 cells transfected with mRNA produced by mutants compared to wild-type, indicating that mRNA synthesized by the wild-type T7 RNAP elicited the strongest immune response, while mRNA from the mutants showed a significantly reduced response.\u003c\/p\u003e\n\u003cp\u003e \u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/T7_low_dsRNA_data_1024x1024.jpg?v=1718656756\" alt=\"Figure 3. The dsRNA content in mRNA synthesized with Hieff™ T7 RNA polymerase is lower than that in mRNA synthesized with the wild-type enzyme after cellulose treatment.\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003eFigure 3. The dsRNA content in mRNA synthesized with Hieff™ T7 RNA polymerase is lower than that in mRNA synthesized with the wild-type enzyme after cellulose treatment.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3 style=\"text-align: left;\"\u003e\u003cstrong\u003e\u003cb\u003eShipping and Storage\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"text-align: left;\"\u003eThe products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.\u003c\/p\u003e\n\u003ch3 style=\"text-align: left;\"\u003e\u003cstrong\u003ePublication:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp class=\"highwire-cite-title\" id=\"page-title\" style=\"text-align: left;\"\u003e\u003ca href=\"https:\/\/www.biorxiv.org\/content\/10.1101\/2024.05.23.595468v2\"\u003e\u003c\/a\u003e\u003ca href=\"https:\/\/febs.onlinelibrary.wiley.com\/doi\/abs\/10.1111\/febs.70051\"\u003eEngineered T7 RNA polymerase reduces dsRNA formation by lowering terminal transferase and RNA-dependent RNA polymerase activities, The FEBS Journal 03 March, 2025 \u003c\/a\u003e\u003ca href=\"https:\/\/www.biorxiv.org\/content\/10.1101\/2024.05.23.595468v2\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp class=\"highwire-cite-title\" style=\"text-align: left;\"\u003e\u003ca href=\"https:\/\/www.biorxiv.org\/content\/10.1101\/2024.05.23.595468v2\"\u003eFADS and semi-rational design modified T7 RNA polymerase reduced dsRNA production, with lower terminal transferase and RDRP activities, bioxRxiv, 31 May, 2024\u003c\/a\u003e\u003c\/p\u003e\n\u003cbr\u003e\n\u003ch3 style=\"text-align: left;\"\u003e\u003cstrong\u003eDocuments\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"text-align: left;\"\u003eSafety Data Sheet\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10628-MSDS-HB250724.pdf?v=1753333597\"\u003e10628_MSDS_HB250724.pdf\u003c\/a\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003eManuals\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003ca title=\"download\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10628ES-Manual-Ver.EN20250724.pdf?v=1753333608\"\u003e10628_Manual_Ver.EN20250724.pdf\u003c\/a\u003e\u003c\/p\u003e\n\u003ch3 style=\"text-align: left;\"\u003e\n\u003ca rel=\"noopener\" class=\"down\" title=\"download\" download=\"\" href=\"https:\/\/seas.yeasenbiotech.com\/prod\/doc\/mainDoc\/10625_Manual_HB221111_EN.pdf\" data-v-98c835fc=\"\" target=\"_blank\"\u003e\u003c\/a\u003e\u003cstrong\u003eRelated Blog：\u003c\/strong\u003e\n\u003c\/h3\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003ca href=\"https:\/\/www.yeasenbio.com\/blogs\/news\/low-dsrna-t7-rna-polymerase\"\u003eHieff™ Low dsRNA T7 RNA Polymerase Mutants, Empowering mRNA Vaccine and Therapy Development\u003c\/a\u003e\u003c\/p\u003e\n\u003cbr\u003e\n\u003cul style=\"text-align: left;\"\u003e\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen","offers":[{"title":"10 KU","offer_id":48835209756990,"sku":"10628ES10","price":180.0,"currency_code":"USD","in_stock":true},{"title":"100 KU","offer_id":48835209789758,"sku":"10628ES60","price":1525.0,"currency_code":"USD","in_stock":true},{"title":"Buy in bulk","offer_id":51313921589566,"sku":"10628ES","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/T71.jpg?v=1751345347"},{"product_id":"14606","title":"RNase R  (20 U\/μL) - 14606ES","description":"\u003cp style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003eRibonuclease R (RNase R) from\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e, is a magnesium-dependent 3′→5′ exoribonuclease that\u003cspan\u003e \u003c\/span\u003ecan digest all linear RNAs but does not digest lariat or circular RNA structures. Most cellular RNAs will be digested completely by RNase R, with the exception of tRNAs, 5S RNA and intron lariats. RNase R is used for enrichment of circRNAs in biological samples, identification of intronic lariat sequences, identification of exonic circRNAs, studying alternative splicing.\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003e\u003cstrong data-filtered=\"filtered\"\u003eFeatures  \u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003eGuaranteed efficacy and quality with ISO 13485 certification.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003eAvailable in bulk or tailored configurations.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003eEffectively breaks down linear RNA, while preserving lariat loops and circular RNAs.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003eSelective digestion of linear RNAs enhances the isolation of circular RNAs for protein production or intronic cDNA library construction.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003eOffering GMP-Grade RNAse R (\u003ca href=\"https:\/\/www.yeabio.com\/products\/14615\" data-mce-href=\"https:\/\/www.yeabio.com\/products\/14615\"\u003e14615\u003c\/a\u003e)\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003e\u003cstrong data-filtered=\"filtered\"\u003eSpecificntions\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eSource\u003c\/td\u003e\n\u003ctd\u003eA recombinant \u003cem\u003eE. coli\u003c\/em\u003e strain carrying the RNAse R gene from \u003cem\u003eE. coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOptimum Temperature\u003c\/td\u003e\n\u003ctd\u003e37℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage Buffer\u003c\/td\u003e\n\u003ctd\u003e50 mM Tris-HCl,100 mM NaCl, 0.1 mM EDTA, 0.1% Triton® X-100, 1 mM DTT, 50% glycerol，pH7.5 at 25℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eUnit Definition\u003c\/td\u003e\n\u003ctd\u003eOne unit converts 1 μg of poly-r(A) into acid-soluble nucleotides in 10 minutes at 37°C in 20 mM Tris-HCl (pH 8.0), 100 mM KCl and 0.1 mM MgCl\u003csub\u003e2\u003c\/sub\u003e.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003e\u003cstrong data-filtered=\"filtered\"\u003eApplication\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003eRNase R serves as a fundamental component in circular RNA synthesis (circRNA). CircRNA holds promise for enhancing pharmaceutical stability and biostability. With their covalently closed structures, circRNAs withstand RNA exonucleases. [1,2] RNase R finds widespread application in various studies, including alternative splicing, intron cDNA production, and the isolation of splicing intermediates and lariats.\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable class=\"MsoTableGrid\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eName\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e14606ES72\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e14606ES86\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e14606ES92\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e14606-A\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eRNase R (20 U\/μL)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e12.5 μL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e125 μL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e500 μL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e14606-B\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e10×RNase R Reaction Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e250 μL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e3 × 1 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e10 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003eThis product should be stored at -25~-15℃ for 2 years.\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003e\u003cstrong\u003eInstructions\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003ePrepare the following reaction system in a sterile microcentrifuge tube\u003c\/p\u003e\n\u003ctable style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003ecomponent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003eVolume\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003e10×RNase R Reaction Buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003e2\u003cspan\u003e \u003c\/span\u003eμL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003eRNA sample\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003e1\u003cspan\u003e \u003c\/span\u003eμg\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003eRNase R (20 U\/μL)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003e2-4 U\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003eRNase-free ddH\u003csub\u003e2\u003c\/sub\u003eO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003eUp to 20\u003cspan\u003e \u003c\/span\u003eμL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003eIncubate at 37 ℃ for 10 - 30 minutes.\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003e\n\u003cstrong\u003e\u003cspan\u003eFigures\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/seas.ysbuy.com\/file\/4328\/9736-168648766661942420.jpg\" data-mce-src=\"https:\/\/seas.ysbuy.com\/file\/4328\/9736-168648766661942420.jpg\"\u003e\u003c\/p\u003e\n\u003cp\u003eFigure 1: Linear RNA molecules can be digested by Yeasen RNAse R.\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/seas.ysbuy.com\/file\/22304\/9776-168648813272560473.jpg\" data-mce-src=\"https:\/\/seas.ysbuy.com\/file\/22304\/9776-168648813272560473.jpg\"\u003e\u003c\/p\u003e\n\u003cp\u003eFigure 2: CircRNA can not be digested by Yeasen RNAse R.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003e \u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/h3\u003e\n\u003col\u003e\n\u003cli style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003eThe activity of RNase R requires 0.1-1.0 mM Mg\u003csup\u003e2+\u003c\/sup\u003e;\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003eWith the increase of RNA, the reaction time can be extended and the enzyme amount can be increased appropriately;\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003eThe EDTA content in RNA samples may affect the activity of RNase R.\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003eIncubate at 70 ℃ for 10 minutes can inactivate the enzyme.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch3 style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003e\u003cstrong\u003e Reference\u003c\/strong\u003e\u003c\/h3\u003e\n\u003col\u003e\n\u003cli style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003eQu L, Yi Z, Shen Y, et al. Circular RNA vaccines against SARS-CoV-2 and emerging variants. Cell. 2022;185(10):1728-1744.e16. doi:10.1016\/j.cell.2022.03.044\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003eChen L, Wang C, Sun H, et al. The bioinformatics toolbox for circRNA discovery and analysis. Brief Bioinform. 2021;22(2):1706-1728. doi:10.1093\/bib\/bbaa001\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003e Manuals\u003ca class=\"down\" title=\"download\" download=\"\" href=\"https:\/\/seas.ysbuy.com\/prod\/doc\/mainDoc\/14606ES-RNase%20R%20(20%20U%CE%BCL)-HB230531.pdf\" data-v-98c835fc=\"\" data-mce-href=\"https:\/\/seas.ysbuy.com\/prod\/doc\/mainDoc\/14606ES-RNase%20R%20(20%20U%CE%BCL)-HB230531.pdf\" target=\"_blank\"\u003e\u003cimg alt=\"\" src=\"data:image\/png;base64,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\" data-v-98c835fc=\"\"\u003e \u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/seas.ysbuy.com\/prod\/doc\/mainDoc\/14606ES-RNase%20R%20(20%20U%CE%BCL)-HB230531.pdf\"\u003eRNAse R manual\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca class=\"down\" title=\"download\" download=\"\" href=\"https:\/\/seas.ysbuy.com\/prod\/doc\/mainDoc\/14606ES-RNase%20R%20(20%20U%CE%BCL)-HB230531.pdf\" data-v-98c835fc=\"\" data-mce-href=\"https:\/\/seas.ysbuy.com\/prod\/doc\/mainDoc\/14606ES-RNase%20R%20(20%20U%CE%BCL)-HB230531.pdf\" target=\"_blank\"\u003e \u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Yeasen","offers":[{"title":"250 U","offer_id":49716944109886,"sku":"14606ES72","price":50.0,"currency_code":"USD","in_stock":true},{"title":"2500U","offer_id":49716944142654,"sku":"14606ES86","price":490.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/14615_34876cf0-1095-451e-a21d-c889b589eef5.png?v=1766971527"},{"product_id":"14616","title":"10 × RNase R  Reaction Buffer  GMP-grade _ 14616ES","description":"\u003cdiv data-v-444b0c7e=\"\" class=\"top_tabc proDetail\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp class=\"MsoBodyText\"\u003e\u003cspan\u003eThis  product  is\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eoptimized\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eand\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eefficient for\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003edigesting\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eall\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003elinear\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eRNA\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eby\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eGMP-grade\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eRNase\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eR. It is\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003emainly\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ecompos\u003c\/span\u003e\u003cspan\u003eed\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eof\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ebuffer,salts and magnesium ions required fo\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003er the \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eenzyme.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoBodyText\"\u003e\u003cspan\u003eThis\u003c\/span\u003e\u003cspan\u003e  \u003c\/span\u003e\u003cspan\u003eproduct\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eis\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eprovide\u003c\/span\u003e\u003cspan\u003ed\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ein\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eliquid  form.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003carticle class=\"4ever-article\"\u003e\u003cstrong\u003e\u003cspan data-type=\"text\" style=\"color: rgb(255, 42, 0);\"\u003e\u003cspan data-type=\"leaf\"\u003e10 × RNase R Reaction Buffer GMP-grade (14616ES) is the correct buffer for RNase R (14615ES).\u003c\/span\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/article\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable style=\"width: 100%;\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 17.0845%;\"\u003eComponents No.\u003c\/td\u003e\n\u003ctd style=\"width: 29.5265%;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 13.3705%;\"\u003e14616ES02\u003c\/td\u003e\n\u003ctd style=\"width: 13.3705%;\"\u003e14616ES03\u003c\/td\u003e\n\u003ctd style=\"width: 14.6668%;\"\u003e14616ES08\u003c\/td\u003e\n\u003ctd style=\"width: 12.0742%;\"\u003e14616ES10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 17.0845%;\"\u003e14616\u003c\/td\u003e\n\u003ctd style=\"width: 29.5265%;\"\u003e10×RNase R  Reaction Buffer  GMP-grade \u003c\/td\u003e\n\u003ctd style=\"width: 13.3705%;\"\u003e500\u003cspan\u003e μL\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 13.3705%;\"\u003e1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 14.6668%;\"\u003e5 mL\u003c\/td\u003e\n\u003ctd style=\"width: 12.0742%;\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eShipping and Storage\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003eThis product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for two years.\u003c\/p\u003e\n\u003ch3 class=\"p\"\u003e\n\u003cspan\u003eDocuments:\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp class=\"p\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eManuals\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/14616_10xRNase_R_Reaction_Buffer_GMP-grade-HB240409.pdf?v=1716268080\"\u003e14616\u003cspan\u003e_Manual_HB240409\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen","offers":[{"title":"500 μL","offer_id":49916441755966,"sku":"14616ES02","price":10.0,"currency_code":"USD","in_stock":true},{"title":"1 mL","offer_id":49739129127230,"sku":"14616ES03","price":20.0,"currency_code":"USD","in_stock":true},{"title":"10 mL","offer_id":49739129159998,"sku":"14616ES10","price":205.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/FsC8aAGYnlYodo2TXQ_M5VDK3rQl.png?v=1768375981"},{"product_id":"10615","title":"mRNA Vaccinia Capping Enzyme (10 U\/μL) _ 10615ES","description":"\u003cdiv class=\"top_tabc proDetail\" data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp\u003e\u003cspan\u003eEukaryotes mRNA forms a special structure at the 5'end after transcription, which is the cap structure. The cap structure plays an important role in the stability, transportation, and translation of mRNA. The vaccinia virus capping enzyme is an effective enzyme that can catalyze the formation of the cap structure. It\"s composed of two subunits D1 and D12. It also has RNA triphosphatase activity, guanylate acyltransferase activity, and guanine methyltransferase activity that could connect the 7-methylguanine cap structure (m7Gppp) to the 5'end of the RNA (m7Gppp5'N). Vaccinia virus capping enzyme can cap the RNA in correct direction within one hour when present at a suitable concentration of capping buffer, guanosine triphosphate (GTP), S-adenosylmethionine (SAM), etc.\u003cbr\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eFeature\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eIsolated from a recombinant source\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eTested for the absence of endonucleases, exonucleases, RNases\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eCapping mRNA before translation assays\/in vitro translation\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eLabeling 5´ end of mRNA\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eSource\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eRecombinant E. coli with vaccinia virus capping enzyme gene\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eOptimum Temperature\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e37℃\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eStorage Buffer\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e20 mM Tris-HCl pH 8.0, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton X-100, 50% glycerin\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eUnit Definition\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e1 unit: The amount of enzyme required to incorporate 10 pmol GTP (α- 32P) into a transcript with 80 nucleotides (80 nt) at 37℃ within 1 hour. \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eName\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10615ES84 (2,000 U)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10615ES92 (10,000 U)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10615ES96 (100,000 U)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10615ES99 (5 MU)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003e10615\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003emRNA Vaccinia Capping Enzyme  (10 U\/μL)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e200 μL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e500 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\n\u003cstrong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/h3\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eShipping and Storage\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003emRNA Vaccinia Capping Enzyme GMP-grade products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eFigures\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/seas.ysbuy.com\/file\/759158\/19c5-167031123115462375.png\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eFigure 1. The capping efficiency almost reached 100% by the vaccinia capping system from YEASEN. Nucleic acid mass spectrometry before the cap reaction was shown in Figure 1A. Nucleic acid mass spectrometry after the cap reaction was shown in Figure 1B.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"top_tabc\"\u003e\u003cstrong\u003e\u003cspan class=\"boxTap\" data-v-444b0c7e=\"\"\u003eFAQ\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"top_tabc\" data-v-444b0c7e=\"\"\u003e\n\u003cp\u003eQ: What is the purpose of heating first and then cooling down in the enzymatic cap addition process? It seems quite difficult to achieve heating followed by cooling during large-scale production.\u003c\/p\u003e\n\u003cp\u003eA: The purpose of preheating is to unfold the secondary structure of RNA and bring it to a single-stranded state. If this step is not carried out, it will affect the efficiency of capping. This part is indeed a current technical challenge in production. Some companies now directly predict the secondary structure of RNA when determining the template sequence. The capping enzyme only recognizes the first few nucleotides of mRNA. If it is determined that this structure will not result in the secondary structure of RNA, then heating can be omitted.\u003c\/p\u003e\n\u003cp\u003eQ: How to choose between cap-like analogues and enzymatic cap addition?\u003c\/p\u003e\n\u003cp\u003eA: In industry, the most commonly used method for enzymatic cap addition is to treat IVT products with vaccinia virus cap-adding enzyme, which can modify them into Cap 0 mRNA. The Cap 0 structure can be further modified into Cap 1 (m7GpppmN) under the action of 2'O-methyltransferase. With enzymatic cap addition, the cap addition efficiency can reach over 95%. The co-transcription cap addition method is simple to operate, but because GTP competes with the cap dimer, the cap addition rate of this method is a little lower; both methods have their own advantages and disadvantages.\u003c\/p\u003e\n\u003cp\u003eQ: What are the methods for optimizing the cap?\u003c\/p\u003e\n\u003cp\u003eA: By increasing the amount of VCE, for example, adding 10 ul of 100 U of enzyme activity to a 100 ul system, the main purpose is to enhance the methyltransferase activity of VCE. It can also be achieved by increasing SAM (0.5 mmol) to enhance the guanine transfer activity and increase the cap formation rate. For instance, our new single-strand cap enzyme has a significantly improved cap formation rate. This is because its guanine transfer activity is better than that of traditional VCE. For traditional VCE, the small subunit D12 is prone to precipitation and polymer formation, which makes purification difficult and leads to loss, thus resulting in higher costs and inability to control batches.\u003c\/p\u003e\n\u003cp\u003eQ: For some mRNAs that do not have caps added, what impact will this have on subsequent experiments, such as those involving the production of biological agents?\u003c\/p\u003e\n\u003cp\u003eA: Currently, the industry has not specifically targeted this aspect. In the industry, the main focus is on the efficiency of the cap application. The current best cap application rate is above 80%. Of course, the higher the better. There is still no absolute standard for this.\u003c\/p\u003e\n\u003cp\u003eFor the synthesized mRNA, purification methods can be employed, such as the one-step membrane encapsulation method by Kang*. This significantly reduces the purification process. The traditional method uses Oligo d(T) columns for purification, with good results achieving a recovery rate of 70-80%; poor results have a recovery rate of 40-50%. An additional step of a hydrophobic column can also be added after the Oligo d(T) column.\u003c\/p\u003e\n\u003cp\u003eQ: What are the requirements for the starting position of the template for co-transcriptional cap addition and two-step cap addition?\u003c\/p\u003e\n\u003cp\u003eA: The co-transcription requirement stipulates that the starting position of the template must begin with AGG*.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eThe two-step method does not have any specific requirements for the position. However, our current products have the highest yield for the AGGG starting position, mainly due to the preference of the T7 enzyme.\u003cspan\u003e\u003c\/span\u003e\u003cspan class=\"boxTap\" data-v-444b0c7e=\"\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"top_tabc\"\u003e\u003cstrong\u003e\u003cspan class=\"boxTap\" id=\"tabCBox1\" data-v-444b0c7e=\"\"\u003eDocuments\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv class=\"cwrap\" data-v-444b0c7e=\"\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003eSafety Data Sheet\u003c\/div\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/MSDS-10615-Vaccinia_Capping_Enzyme_10_U__L_-HB220701.pdf?v=1740627307\"\u003e10615_MSDS_HB220701\u003cbr\u003e\u003c\/a\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen","offers":[{"title":"2 KU","offer_id":50861142081854,"sku":"10615ES84","price":365.0,"currency_code":"USD","in_stock":true},{"title":"10 KU","offer_id":50861142114622,"sku":"10615ES92","price":1445.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10615.png?v=1766971422"},{"product_id":"10629","title":"Hieff™ T7 RNA polymerase GMP-grade  (low dsRNA,250 U\/μL) _ 10629ES","description":"\u003cdiv class=\"top_tabc proDetail\" data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp\u003eThis is an engineered T7 RNA polymerase variant (low dsRNA) derived from the wild-type T7 RNA polymerase and produced in Escherichia coli. It significantly reduces the production of double-stranded RNA (dsRNA) while efficiently incorporating cap analogs, and exhibiting highly efficient in vitro transcription (IVT) comparable to the wild-type T7 RNA polymerase. It catalyzes the 5'→3' synthesis of RNA on double-stranded DNA from its T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') and uses NTPs as substrates.\u003c\/p\u003e\n\u003cp\u003eNote: G* is the first base of the RNA transcript.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eFeature\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003edsRNA level lower to about 1\/100000\u003c\/li\u003e\n\u003cli\u003eComptiable with Trilink CleanCap AG, LZCap\u003c\/li\u003e\n\u003cli\u003eHigh yields comparable to WT \u003c\/li\u003e\n\u003cli\u003eLower Cap input\u003c\/li\u003e\n\u003cli\u003eAnimal origin-free (AOF)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeabio.com\/blogs\/news\/low-dsrna-t7-rna-polymerase\"\u003e\u003cspan\u003ePlease find information on the development of this enzyme.\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003e\u003cb\u003eComponents\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable cellspacing=\"2\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"170\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"261\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"177\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10629ES10\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"172\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10629ES60\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"156\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10629ES72\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"196\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10629ES86\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"170\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"261\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"177\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e(10 KU)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"172\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e(100 KU)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"156\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e(250 KU)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"196\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e(2,500 KU)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"170\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10629\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"261\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHieff™ T7 RNA Polymerase (GMP Grrade, low dsRNA, 250 U\/μL)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"177\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e40 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"172\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e400 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"156\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"196\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cstrong\u003e\u003cb\u003eSpecifications \u003c\/b\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"301\"\u003e\n\u003cp\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"982\"\u003e\n\u003cp\u003eRecombinant\u003cem\u003e\u003ci\u003e \u003c\/i\u003e\u003c\/em\u003e\u003cem\u003e\u003ci\u003eE. coli \u003c\/i\u003e\u003c\/em\u003ewith T7 RNA Polymerase gene\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"301\"\u003e\n\u003cp\u003e\u003cstrong\u003eOptimum Temperature\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"982\"\u003e\n\u003cp\u003e37℃\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"301\"\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"982\"\u003e\n\u003cp\u003e50 mM Tris-HCl, 1 mM EDTA, 10 mM DTT, 100 mM NaCl, 0.1% Triton X-100，50% (v\/v) glycerin，pH7.9 at 25℃\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"301\"\u003e\n\u003cp\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"982\"\u003e\n\u003cp\u003eThe amount of enzyme required to incorporate 1 nmol of [\u003csup\u003e3\u003c\/sup\u003eH] GMP into the acid-insoluble precipitate within 1 hour at 37°C and pH 8.0 is defined as 1 unit.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eRecommended Mg2+\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e30mM Magnesium Acetate\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\n\u003cstrong\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/h3\u003e\n\u003ch3\u003e\n\u003cstrong\u003e\u003cb\u003eQC \u003cspan\u003eStandard\u003c\/span\u003e\u003c\/b\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/strong\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"425\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003e\u003cspan style=\"font-family: Calibri;\"\u003eI\u003c\/span\u003etems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"731\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eSpecification\/Standard\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"425\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eEnzyme\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eactivity\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"731\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e250\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003e-\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e300U\/μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"425\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eProtein Purity\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"731\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003e≥95%\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"425\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eEndotoxin\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"731\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u0026lt;20 EU\/mg\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"425\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eProtease\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"731\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eNegative\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"425\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eExonuclease\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"731\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eNegative\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"425\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eNickase\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"731\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eNegative\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"425\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eRNase\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"731\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eNegative\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"425\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eE.coli host protein\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"731\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u0026lt;50ppm\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"425\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eE\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e.coli host\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eDNA\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"731\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u0026lt;10 fg\/U\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"425\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eMycoplasma examination\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"731\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eNegative\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cstrong\u003e\u003cb\u003eRelated Products\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeabio.com\/blogs\/mrna\/gmp-grade-enzymes\"\u003e\u003cspan\u003eTris NTPs synergistically decreasing dsRNA\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeabio.com\/products\/10670\"\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e10× Transcription Buffer 2 GMP-grade 10670ES\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e\u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e\u003c\/span\u003e\u003ca href=\"https:\/\/www.yeabio.com\/products\/10657\"\u003e\u003cspan style=\"font-size: 0.875rem;\" data-v-9e66f18e=\"\"\u003eN1-Me-Pseudo UTP Tris Solution GMP-grade (100 mM) \u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\" data-v-9e66f18e=\"\" class=\"code\"\u003e10657ES\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeabio.com\/products\/10656\"\u003e\u003cspan data-v-9e66f18e=\"\" style=\"font-size: 0.875rem;\"\u003ePseudo UTP Tris Solution GMP-grade (100 mM) \u003c\/span\u003e\u003cspan data-v-9e66f18e=\"\" class=\"code\" style=\"font-size: 0.875rem;\"\u003e10656ES\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeabio.com\/products\/10652\"\u003e\u003cspan data-v-9e66f18e=\"\" style=\"font-size: 0.875rem;\"\u003eATP Tris Solution GMP-grade (100 mM) \u003c\/span\u003e\u003cspan data-v-9e66f18e=\"\" class=\"code\" style=\"font-size: 0.875rem;\"\u003e10652ES\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeabio.com\/products\/10655\"\u003e\u003cspan data-v-9e66f18e=\"\" style=\"font-size: 0.875rem;\"\u003eGTP Tris Solution GMP-grade (100 mM) \u003c\/span\u003e\u003cspan data-v-9e66f18e=\"\" class=\"code\" style=\"font-size: 0.875rem;\"\u003e10655ES\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeabio.com\/products\/10653\"\u003e\u003cspan data-v-9e66f18e=\"\" style=\"font-size: 0.875rem;\"\u003eCTP Tris Solution GMP-grade (100 mM) \u003c\/span\u003e\u003cspan data-v-9e66f18e=\"\" class=\"code\" style=\"font-size: 0.875rem;\"\u003e10653ES\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeabio.com\/products\/10654\"\u003e\u003cspan style=\"font-size: 0.875rem;\" data-v-9e66f18e=\"\"\u003eUTP Tris Solution GMP-grade (100 mM) \u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\" data-v-9e66f18e=\"\" class=\"code\"\u003e10654ES\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeabio.com\/products\/36717\"\u003e\u003cspan data-v-9e66f18e=\"\" style=\"font-size: 0.875rem;\"\u003eDouble-stranded RNA (dsRNA) ELISA kit 36717ES\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003ch3\u003e\u003cstrong\u003e\u003cb\u003eFigures\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eCustomer feedback:\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/dsRNA_figure_68789415-4b42-4894-84b5-a93b08e8d406.jpg?v=1714285432\" alt=\"\"\u003e\u003c\/div\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cspan style=\"font-size: 0.875rem;\"\u003eFigure 1. Testing of T7 RNA Polymerase mutants. dsRNA Content: The dsRNA content reduced by more than 10-fold (A).  mRNA Integrity: Maintained at over 90% (B).  2K Fragment Capping Efficiency: Exceeded 99% (C, D).\u003c\/span\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cspan style=\"font-size: 0.875rem;\"\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003cdiv data-v-444b0c7e=\"\" style=\"text-align: start;\"\u003e\n\u003cdiv data-v-9e66f18e=\"\" class=\"ListTitle\"\u003e\n\u003cdiv data-v-9e66f18e=\"\"\u003e\n\u003cdiv data-v-9e66f18e=\"\" class=\"ListTitle\"\u003e\n\u003cspan data-v-9e66f18e=\"\" class=\"code\"\u003e\u003cspan data-v-9e66f18e=\"\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/Yields.jpg?v=1714284938\" alt=\"\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003ca data-v-9e66f18e=\"\" href=\"https:\/\/www.yeasenbiotech.com\/productdetail\/2858\"\u003e\u003cbr\u003e\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv data-v-9e66f18e=\"\" class=\"ListTitle\"\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eTable 1. The Yields of low dsRNA T7 RNA polymerase is comparable with that of WT.\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv data-v-9e66f18e=\"\" class=\"ListTitle\"\u003e\n\u003cspan style=\"font-size: 0.875rem;\"\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/t61_1024x1024.png?v=1717802537\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/h2\u003e\n\u003cp\u003eFigure 2. Evaluation of the immunogenicity of the IVT products in murine RAW264.7 cells (Figures 2A and 2B). IFN-β mRNA and protein levels were reduced in RAW264.7 cells transfected with mRNA produced by mutants compared to wild-type, indicating that mRNA synthesized by the wild-type T7 RNAP elicited the strongest immune response, while mRNA from the mutants showed a significantly reduced response.\u003c\/p\u003e\n\u003cp\u003e \u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/T7_low_dsRNA_data_1024x1024.jpg?v=1718656756\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003eFigure 3. The dsRNA content in mRNA synthesized with CleaScript™ T7 RNA polymerase is lower than that in mRNA synthesized with the wild-type enzyme after cellulose treatment.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003e\u003cb\u003eShipping and Storage\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eThe products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003ePublication:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp id=\"page-title\" class=\"highwire-cite-title\"\u003e\u003ca href=\"https:\/\/febs.onlinelibrary.wiley.com\/doi\/abs\/10.1111\/febs.70051\"\u003eEngineered T7 RNA polymerase reduces dsRNA formation by lowering terminal transferase and RNA-dependent RNA polymerase activities, The FEBS Journal 03 March, 2025 \u003c\/a\u003e\u003ca href=\"https:\/\/www.biorxiv.org\/content\/10.1101\/2024.05.23.595468v2\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp class=\"highwire-cite-title\"\u003e\u003ca href=\"https:\/\/www.biorxiv.org\/content\/10.1101\/2024.05.23.595468v2\"\u003eFADS and semi-rational design modified T7 RNA polymerase reduced dsRNA production, with lower terminal transferase and RDRP activities, bioxRxiv, 31 May, 2024\u003c\/a\u003e\u003c\/p\u003e\n\u003cbr\u003e\n\u003ch3\u003e\u003cstrong\u003eDocuments\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eSafety Data Sheet\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10629-MSDS-HB250708.pdf?v=1751959192\"\u003e10629_MSDS_HB250708\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eManuals\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10629ES-Manual-Ver.EN20250609_1_94a26b49-1d11-4058-9923-8296ff780e2b.pdf?v=1749775294\"\u003e\u003cspan\u003e10629_Manual_Ver.CN20250609\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen","offers":[{"title":"10 KU","offer_id":50955054711102,"sku":"10629ES10","price":198.0,"currency_code":"USD","in_stock":true},{"title":"100 KU","offer_id":50955054743870,"sku":"10629ES60","price":1675.0,"currency_code":"USD","in_stock":true},{"title":"Buy in bulk","offer_id":51314017567038,"sku":"10629ES","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/T71.jpg?v=1751345347"},{"product_id":"10635","title":"T7 High Yield RNA Synthesis Kit for Co-transcription (low dsRNA, with LZCap AG) _ 10635ES","description":"\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThe kit utilizes \u003c\/span\u003e\u003cspan\u003eT7 RNA Polymerase (Low dsRNA) and high-affinity LZcap AG Cap1 analog to produce mRNAs with significantly reduced double-stranded RNA (dsRNA) content and enhanced expression. Single-stranded RNAs are synthesized using linear double-stranded DNA containing a T7 promoter sequence as the template. Modified nucleotides, such as biotin or dye-labeled nucleotides, can be incorporated during transcription to generate labeled RNA. The kit supports the synthesis of both long and short transcripts, yielding 100–200 μg of RNA from 1 μg of DNA template. Substituting UTP with N1-Me-Pseudo UTP reduces RNA immunogenicity. The resulting RNA is suitable for downstream applications, including RNA structure and function studies, RNase protection, probe hybridization, RNAi, microinjection, and in vitro translation.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ctable class=\"MsoNormalTable\" border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"135\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"444\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e635\u003c\/span\u003e\u003cspan\u003eES\u003c\/span\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e(\u003c\/span\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e0 \u003c\/span\u003e\u003cspan\u003eT\u003c\/span\u003e\u003cspan\u003e)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"173\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e635\u003c\/span\u003e\u003cspan\u003eES\u003c\/span\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(50 \u003c\/span\u003e\u003cspan\u003eT\u003c\/span\u003e\u003cspan\u003e)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e635\u003c\/span\u003e\u003cspan\u003eES\u003c\/span\u003e\u003cspan\u003e60\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e(\u003c\/span\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e00 \u003c\/span\u003e\u003cspan\u003eT\u003c\/span\u003e\u003cspan\u003e)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"173\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e635\u003c\/span\u003e\u003cspan\u003eES\u003c\/span\u003e\u003cspan\u003e70\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e(\u003c\/span\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e00 \u003c\/span\u003e\u003cspan\u003eT\u003c\/span\u003e\u003cspan\u003e)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"135\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e106\u003c\/span\u003e\u003cspan\u003e35-A\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"444\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eT7 RNA Polymerase \u003c\/span\u003e\u003cspan\u003eMix\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e20 \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"173\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e100 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e00 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"173\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003em\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"135\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e106\u003c\/span\u003e\u003cspan\u003e35-B\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"444\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10×\u003c\/span\u003e\u003cspan\u003eTranscription\u003c\/span\u003e\u003cspan\u003e Buffer\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e20 \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"173\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e00 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e00 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"173\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003em\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"135\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10635-C\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"444\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eATP\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e（\u003c\/span\u003e\u003cspan style=\"font-family: Inter;\"\u003e100mM\u003c\/span\u003e\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e）\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e20 \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"173\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e100 \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e00 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"173\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003em\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"135\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10635-D\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"444\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eCTP\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e（\u003c\/span\u003e\u003cspan style=\"font-family: Inter;\"\u003e100mM\u003c\/span\u003e\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e）\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e20 \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"173\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e100 \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e00 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"173\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003em\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"135\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10635-E\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"444\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eGTP\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e（\u003c\/span\u003e\u003cspan style=\"font-family: Inter;\"\u003e100mM\u003c\/span\u003e\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e）\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e20 \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"173\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e100 \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e00 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"173\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003em\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"135\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10635-F\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"444\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eN\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e-Me-Pseudo UTP\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e（\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e100mM\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e）\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e20 \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"173\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e100 \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e00 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"173\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003em\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"135\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10635-G\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"444\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eControl DNA Template\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e（\u003c\/span\u003e\u003cspan style=\"font-family: Inter;\"\u003e500ng\/\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e）\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e5 \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"173\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e0 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"173\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e0 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"135\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10635-H\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"444\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eDNase I (2 U\/μL)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e10 \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"173\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e100 \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"173\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e500 \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"135\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10635-I\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"444\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eC\u003c\/span\u003e\u003cspan\u003eap1 Analog \u003c\/span\u003e\u003cspan\u003e(100 mM)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e20 \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"173\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e100 \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e200 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"173\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"135\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10635-J\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"444\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eLithium chloride solution\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e300 \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"173\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e1.5 m\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e3 m\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"173\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e15 m\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"135\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10635-K\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"444\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eRNase-free ddH\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003eO\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e500 \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"173\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e2.5 m\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e5 m\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"173\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e25 m\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eNote:\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e The \u003c\/span\u003e\u003cspan\u003eT7 RNA Polymerase \u003c\/span\u003e\u003cspan\u003eMix\u003c\/span\u003e\u003cspan\u003e includes \u003c\/span\u003e\u003cspan\u003e\u003ca href=\"\/ar\/products\/10628\"\u003eT7 RNA polymerase (Low dsRNA\u003c\/a\u003e, Cat: 10628), \u003c\/span\u003e\u003cspan\u003e\u003ca href=\"https:\/\/www.yeasenbio.com\/products\/10621\"\u003eMurine RNase inhibitor\u003c\/a\u003e\u003c\/span\u003e\u003cspan\u003e (Cat: 10621) and \u003c\/span\u003e\u003cspan\u003e\u003ca href=\"https:\/\/www.yeasenbio.com\/products\/10620\"\u003ePyrophosphatase Inorganic\u003c\/a\u003e\u003c\/span\u003e\u003cspan\u003e (Cat: 10620); The \u003c\/span\u003e\u003cspan\u003eC\u003c\/span\u003e\u003cspan\u003eap1 Analog \u003c\/span\u003e\u003cspan\u003eis \u003ca href=\"\/ar\/products\/10684\"\u003eLZcap AG\u003c\/a\u003e \u003c\/span\u003e\u003cspan\u003e(\u003c\/span\u003e\u003cspan\u003eCat: 10684\u003c\/span\u003e\u003cspan\u003e)\u003c\/span\u003e\u003cspan\u003e. The catalog number of recommended \u003ca href=\"\/ar\/products\/10654\"\u003eUTP \u003c\/a\u003eand\u003ca href=\"\/ar\/products\/10656\"\u003e Pseudo UTP\u003c\/a\u003e is 10654 and 10656, respectively.   \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eFeatures \u003c\/span\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003cstrong\u003eComplete Kit Components:\u003c\/strong\u003e Includes all essential reagents for one-step co-transcriptional capping, such as NTPs, T7 Enzyme Mix, cap analog, DNase I, and LiCl.\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003cstrong\u003eSimplified Workflow:\u003c\/strong\u003e Utilizes a co-transcriptional capping process, minimizing the number of components introduced and streamlining the overall procedure for ease of use.\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003cstrong\u003eHigh Yield: \u003c\/strong\u003eGenerates 150–200 µg of Cap 1 mRNA from just 1 µg of DNA template.\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003cstrong\u003eReduced Immunogenicity:\u003c\/strong\u003e Incorporates modified N1-Me-pUTP and a low dsRNA T7 RNA polymerase to minimize unintended innate immune responses.\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003cstrong\u003eHigh Capping Efficiency:\u003c\/strong\u003e Achieves capping efficiency exceeding 99%.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eFigure\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10684.jpg?v=1747816023\" alt=\"\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003e1. Broad Applicability:  \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eConsistently delivers a high yield of 9 mg\/mL with capping efficiency up to 100% in a 20 µL reaction system using 1 µg of plasmid DNA, regardless of the length of the target transcript.\u003c\/p\u003e\n\u003ctable width=\"716\" style=\"width: 537pt; height: 186pt; border-collapse: collapse;\" cellspacing=\"0\" cellpadding=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 30pt;\" height=\"39\"\u003e\n\u003ctd width=\"179\" style=\"width: 134pt;\" class=\"kso0\"\u003e\n\u003cp\u003emRNA Category\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"179\"\u003e\n\u003cp\u003eYield(mg\/mL)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"179\"\u003e\n\u003cp\u003emRNA Integrity(%)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"179\" style=\"width: 134pt;\" class=\"kso3\"\u003e\n\u003cp\u003eCapping Efficiency(%)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 30pt;\" height=\"39\"\u003e\n\u003ctd width=\"179\" style=\"width: 134pt;\" class=\"kso4\"\u003e\n\u003cp\u003eeGFP\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"179\"\u003e\n\u003cp\u003e11.2\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"179\"\u003e\n\u003cp\u003e93.5\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"179\" style=\"width: 134pt;\" class=\"kso7\"\u003e\n\u003cp\u003e100%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 30pt;\" height=\"39\"\u003e\n\u003ctd width=\"179\" style=\"width: 134pt;\" class=\"kso8\"\u003e\n\u003cp\u003eOVA\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"179\"\u003e\n\u003cp\u003e10.2\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"179\"\u003e\n\u003cp\u003e94.3\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"179\" style=\"width: 134pt;\" class=\"kso11\"\u003e\n\u003cp\u003e100%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 30pt;\" height=\"39\"\u003e\n\u003ctd width=\"179\" style=\"width: 134pt;\" class=\"kso12\"\u003e\n\u003cp\u003emcherry\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"179\"\u003e\n\u003cp\u003e9.1\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"179\"\u003e\n\u003cp\u003e90.3\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"179\" style=\"width: 134pt;\" class=\"kso15\"\u003e\n\u003cp\u003e100%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 30pt;\" height=\"39\"\u003e\n\u003ctd width=\"179\" style=\"width: 134pt;\" class=\"kso16\"\u003e\n\u003cp\u003eLuciferase\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"179\"\u003e\n\u003cp\u003e9.3\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"179\"\u003e\n\u003cp\u003e90.1\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"179\" style=\"width: 134pt;\" class=\"kso19\"\u003e\n\u003cp\u003e100%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 30pt;\" height=\"39\"\u003e\n\u003ctd width=\"179\" style=\"width: 134pt;\" class=\"kso20\"\u003e\n\u003cp\u003eCas 9\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"179\"\u003e\n\u003cp\u003e9\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"179\"\u003e\n\u003cp\u003e88.7\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"179\" style=\"width: 134pt;\" class=\"kso23\"\u003e\n\u003cp\u003e100%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp style=\"text-align: center;\"\u003eTable 1. Broad applicability of the Co-transcription system\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"79\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"304\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003eCap analog\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"204\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003eCap Concn. (mM)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"182\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003eYields (mg\/mL)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"185\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003eIntegrity (%)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"420\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003eCapping efficiency by MS (%)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"79\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e1\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"304\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003eCap AG    (3'OMe-7mG)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"204\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e10\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"182\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e12.69\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"185\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e80.6\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"420\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e100\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"79\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e2\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"304\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003eLZCap AG\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"204\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e10\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"182\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e12.11\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"185\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e82.8\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"420\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e100\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"79\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e3\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"304\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003eLZCap AG\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"204\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e7.5\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"182\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e11.59\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"185\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e82.2\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"420\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e100\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"79\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e4\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"304\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003eLZCap AG\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"204\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e5\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"182\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e11.16\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"185\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e81.6\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"420\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e100\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"79\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e5\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"304\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003eLZCap AG\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"204\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e2.5\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"182\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e10.75\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"185\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e81.4\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"420\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e100\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg style=\"float: none;\" alt=\"Figure 1.\u0026amp;nbsp;Co-transcription analysis of the input of LZcap AG. IVT materials include Hieff™ T7 RNA Polymerase,\u0026amp;nbsp;Template: Cas9\u0026amp;nbsp;(4K); T7 Reaction Buffer 2; Tris NTP (including N1-Me-Pseudo UTP Tris Solution).\u0026amp;nbsp; IVT time: 3 hrs.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10635-2_1024x1024.jpg?v=1747830350\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: center;\" class=\"MsoNormal\"\u003eFigure 1. \u003cspan\u003eCo-transcription analysis of the input of LZcap AG. IVT materials include Hieff™ T7 \u003cspan style=\"font-family: Inter 18pt Medium;\"\u003eRNA Polymerase,\u003c\/span\u003e\u003cb\u003e \u003c\/b\u003eTemplate: Cas9 \u003cspan style=\"font-family: Inter 18pt Medium;\"\u003e(4K)\u003c\/span\u003e; T7 Reaction Buffer 2; Tris NTP (including N1-Me-Pseudo UTP Tris Solution).  IVT time: 3 hrs.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"173\" valign=\"center\"\u003e\n\u003cp class=\"p\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eFragment Length\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"297\" valign=\"center\"\u003e\n\u003cp class=\"p\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eT7 RNA pol\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"196\" valign=\"center\"\u003e\n\u003cp class=\"p\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eYield\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003cspan\u003e \u003c\/span\u003e(\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003emg\/mL\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"233\" valign=\"center\"\u003e\n\u003cp class=\"p\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e Integrity\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003cspan\u003e \u003c\/span\u003e(\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e%\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"299\" valign=\"center\"\u003e\n\u003cp class=\"p\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003edsRNA Content (ng\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\/\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eug of RNA)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"173\" valign=\"center\" rowspan=\"2\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e4K\u003c\/span\u003e\u003cspan\u003e mRNA\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"297\" valign=\"center\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eRegular T7 Kit\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"196\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e12.4\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"233\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e88.3\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"299\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e0.4273\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"297\" valign=\"center\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eLow dsRNA T7 \u003c\/span\u003e\u003cspan\u003eKit \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"196\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e12.1\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"233\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e89.9\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"299\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e0.0129\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"173\" valign=\"center\" rowspan=\"2\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e9K\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003emRNA\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"297\" valign=\"center\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eRegular T7 Kit\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"196\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e9.5\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"233\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e81.9\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"299\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e2.9180\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"297\" valign=\"center\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eLow dsRNA T7 Kit \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"196\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e9.0\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"233\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e82.0\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"299\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e0.0379\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align: center;\" class=\"MsoNormal\"\u003eTable 2. dsRNA content analysis of regular T7 Kit and Low dsRNA T7 Kit.\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThis product should be stored at -25\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e~-15℃ for \u003c\/span\u003e\u003cspan\u003eone\u003c\/span\u003e\u003cspan\u003e year.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eNotes: \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"15\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e1. \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eBe careful not to mix RNase in the reaction system.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"15\"\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e2. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eExperiment equipment (such as: pipette tip, product tube, etc.) should strictly use RNase-free products.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"15\"\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e3. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eFor your safety and health, please wear lab coats and disposable gloves. \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"15\"\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e4. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eFor research use only!\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eDocument\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eSafety Data Sheet\u003c\/p\u003e\n\u003cp\u003e10635_MSDS\u003c\/p\u003e\n\u003cp class=\"15\"\u003e\u003cspan\u003eManual\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"15\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10635-Manual-EN20250703.pdf?v=1751507980\"\u003e\u003cspan\u003e10635_Manual_Ver.EN20250703\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Yeasen","offers":[{"title":"10 T","offer_id":51747233530174,"sku":"10635ES10","price":165.0,"currency_code":"USD","in_stock":true},{"title":"50 T","offer_id":51747233562942,"sku":"10635ES50","price":650.0,"currency_code":"USD","in_stock":true},{"title":"100 T","offer_id":51747233595710,"sku":"10635ES60","price":1245.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10623_10c316e0-21a8-457a-977d-21d30b683188.png?v=1766969927"},{"product_id":"10668","title":"10× Digestion buffer 4 GMP-grade _ 10668ES","description":"\u003cdiv data-v-444b0c7e=\"\" class=\"top_tabc proDetail\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp class=\"H3_backgro\"\u003eThis product is an optimized 10×Digestion buffer for different restriction enzymes to ensure optimal (100%) digestion activity.  This product is produced in accordance with GMP process requirements and provided in a liquid form.\u003cbr\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eBuffer for different restriction enzymes to ensure optimal (100%) digestion activity\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eBsa I digestion buffer\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eFormat\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eLiquid\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eConcentration\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10X\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eFormulation\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp dir=\"auto\"\u003e500 mM potassium acetate, 200 mM Tris-acetate, 100 mM magnesium acetate, 1 mg\/ml OsrHSA, pH 7.9 at 25°C.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eName\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10667ES03\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10667ES10\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10667ES50\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003e10668\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10×Digestion buffer 4 GMP-grade\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e50 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eShipping and Storage\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003eThe product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"top_tabc\"\u003e\u003cstrong\u003e\u003cspan data-v-444b0c7e=\"\" id=\"tabCBox1\" class=\"boxTap\"\u003eDocuments:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan data-v-444b0c7e=\"\" class=\"boxTap\"\u003eManuals\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan data-v-444b0c7e=\"\" class=\"boxTap\"\u003eMSDS\u003c\/span\u003e\u003c\/p\u003e","brand":"Yeasen","offers":[{"title":"1 mL","offer_id":51781665587518,"sku":"10668ES03","price":55.0,"currency_code":"USD","in_stock":true},{"title":"10 mL","offer_id":51781665620286,"sku":"10668ES10","price":205.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":51781665653054,"sku":"10668ES50","price":825.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/FnI9Zhj0G2q9AO8FDmOlk2MO9InM.png?v=1768375549"},{"product_id":"14810","title":"SP6 RNA Polymerase (20 U\/μL) _ 14810ES","description":"\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eSP6 Bacteriophage RNA Polymerase is a DNA-dependent RNA polymerase that exhibits high specificity for the SP6 bacteriophage promoter. It catalyzes the incorporation of NTPs downstream of the SP6 promoter on single- or double-stranded DNA templates, synthesizing RNA complementary to the DNA template downstream of the SP6 promoter. This enzyme not only performs conventional RNA synthesis but also recognizes modified NTPs, such as biotin-, digoxigenin-, or fluorescein-labeled NTPs, making it suitable for a wide range of research applications and biotechnological uses.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eFeatures\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eHigh Purity:\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e Free from exonuclease, endonuclease, non-specific nuclease, and RNase contamination, ensuring clean and reliable transcription.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eHigh Transcription Efficiency:\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e Delivers robust in vitro RNA synthesis performance comparable to leading international brands.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eRecombinant Source: \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003eProduced in recombinant \u003c\/span\u003e\u003ci\u003e\u003cspan\u003eE. coli\u003c\/span\u003e\u003c\/i\u003e\u003cspan\u003e expressing the Salmonella typhimurium SP6 RNA polymerase gene.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eDefined P\u003c\/span\u003e\u003c\/b\u003e\u003cstrong\u003ero\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003emoter Recognition: \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003eRecognizes the SP6 promoter sequence (5′-ATTTAGGTGACACTATAGAAGNG-3′) for precise and efficient transcription initiation.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"167\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"356\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"149\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e14810ES84\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"149\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e14810ES92\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"167\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e14810-A\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"356\"\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan\u003eSP6 RNA Polymerase (20 U\/μL)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"149\"\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan\u003e100 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"149\"\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan\u003e500 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"167\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e14810-B\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"356\"\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan\u003e10×SP6 Reaction Buffer\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"149\"\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan\u003e500 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"149\"\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan\u003e2×1 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eStorage\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp\u003eThis product should be stored at -25~-15℃ for 2 years.\u003c\/p\u003e\n\u003ch3 class=\"p\" align=\"justify\"\u003e\n\u003cstrong\u003eApplication\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli class=\"p\" align=\"justify\"\u003e\n\u003cspan\u003ePreparation of radiolabeled RNA probes\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"p\" align=\"justify\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cspan\u003eNon-isotopic RNA labeling\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"p\" align=\"justify\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cspan\u003eRNA vaccine preparation\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"p\" align=\"justify\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cspan\u003eGuide RNA for target genes\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"p\" align=\"justify\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cspan\u003emRNA for in vitro translation and microinjection\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"p\" align=\"justify\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cspan\u003eRNA for structural, processing, and catalytic studies\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"p\" align=\"justify\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cspan\u003eRNA amplification\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"p\" align=\"justify\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cspan\u003eSynthesis of RNA antisense strands for gene expression regulation\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"p\" align=\"justify\"\u003e\n\u003cstrong\u003eFigures\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"p\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e1. \u003c\/span\u003eHigh Transcription Efficiency\u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" alt=\"Figure 1. In vitro transcription efficiency of Yeasen SP6 RNA Polymerase\u0026amp;nbsp;compared with a leading brand.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/1_cb36a557-48d7-4ac4-beaf-22c31fda8682_1024x1024.png?v=1760505786\"\u003e\u003c\/div\u003e\n\u003cp class=\"p\" align=\"center\"\u003eFigure 1. In vitro transcription efficiency of Yeasen SP6 RNA Polymerase compared with a leading brand.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eIn vitro transcription was performed using a linearized plasmid DNA template containing the SP6 promoter. Reactions included varying amounts of Yeasen SP6 RNA Polymerase or a leading brand's enzyme. After incubation at 37°C for 2 h, reactions were treated with DNase I to remove template DNA. The resulting 248 nt RNA transcripts were analyzed by 1% agarose gel electrophoresis in 1× TAE buffer and visualized by staining.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e2. \u003c\/span\u003e\u003c!--[endif]--\u003eHigh Purity\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" alt=\"Figure 1. Purity assessment of Yeasen SP6 RNA Polymerase\u0026amp;nbsp;for exonuclease, nickase, nonspecific nuclease, and RNase contamination.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/2_daafbf5d-cbb1-4c5d-be52-43112c95dd2f_1024x1024.png?v=1760505786\"\u003e\u003c\/div\u003e\n\u003cp class=\"p\" align=\"center\"\u003eFigure 2. Purity assessment of Yeasen SP6 RNA Polymerase for exonuclease, nickase, nonspecific nuclease, and RNase contamination.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003eSP6 RNA Polymerase was incubated with nucleic acid substrates and analyzed by agarose gel electrophoresis to observe any band changes. The results demonstrate that all three batches of Yeasen SP6 RNA Polymerase tested negative for exonuclease (100 U), nickase (100 U), nonspecific nuclease (100 U), and RNase (20 U) contaminants. This ensures the accuracy and reliability of your experimental outcomes.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"p\"\u003e\n\u003cb\u003e\u003cspan\u003eDocuments:\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSafety Data Sheet\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/14810-MSDS-HB251015.pdf?v=1760505882\"\u003e14810_MSDS_HB251015\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eManuals\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/14810ES-Manual-Ver.EN20251011.pdf?v=1760505881\"\u003e14810_Manual_Ver.EN20251011\u003c\/a\u003e\u003c\/p\u003e","brand":"Yeasen","offers":[{"title":"2000 U","offer_id":52334956773694,"sku":"14810ES84","price":85.0,"currency_code":"USD","in_stock":true},{"title":"10000 U","offer_id":52334956806462,"sku":"14810ES92","price":295.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/d7bfa02db7ad49c7a977f73dc6e7e8c3.png?v=1764214867"},{"product_id":"10674","title":"10× Transcription Buffer 3 (GMP-Grade)_ 10674ES","description":"\u003cp align=\"justify\"\u003eThis product is optimized transcription buffer and efficient for in vitro synthesis of RNA. This product is mainly composed of buffer salts, reducing agents and magnesium ions required for enzyme proteins.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable cellspacing=\"9\" border=\"0\" class=\"MsoNormalTable\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"119\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eCat.No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"664\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e10674ES03 \/ 10674ES10 \/ 10674ES25\/ 10674ES60 \/ 10674ES76\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"119\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eSize\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"664\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e1 mL \/ 10 mL \/ 25 mL \/ 100 mL \/ 500 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable style=\"width: 108.54%; height: 187px;\" cellspacing=\"9\" border=\"0\" class=\"MsoNormalTable\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 55.1944px;\"\u003e\n\u003ctd style=\"width: 14.4204%; height: 55.1944px;\" valign=\"top\" width=\"62\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.4204%; height: 55.1944px;\" valign=\"top\" width=\"127\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.3845%; height: 55.1944px;\" valign=\"top\" width=\"118\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e10674ES03\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.3845%; height: 55.1944px;\" valign=\"top\" width=\"118\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e10674ES10\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.3845%; height: 55.1944px;\" valign=\"top\" width=\"118\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e10674ES25\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.3845%; height: 55.1944px;\" valign=\"top\" width=\"119\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e10674ES60\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.3845%; height: 55.1944px;\" valign=\"top\" width=\"114\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e10674ES76\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 97.9861px;\"\u003e\n\u003ctd style=\"width: 14.4204%; height: 97.9861px;\" valign=\"top\" width=\"62\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e10674\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.4204%; height: 97.9861px;\" valign=\"center\" width=\"127\"\u003e\n\u003carticle class=\"4ever-article\"\u003e\u003cspan\u003e10× Transcription Buffer 3 \u003cspan data-type=\"text\"\u003e\u003cspan data-type=\"leaf\"\u003eGMP-Grade\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/article\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.3845%; height: 97.9861px;\" valign=\"center\" width=\"118\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.3845%; height: 97.9861px;\" valign=\"center\" width=\"118\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e10 mL \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.3845%; height: 97.9861px;\" valign=\"center\" width=\"118\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e25 mL \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.3845%; height: 97.9861px;\" valign=\"center\" width=\"119\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e100 mL \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.3845%; height: 97.9861px;\" valign=\"center\" width=\"114\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e500 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eThis product should be stored at -25~-15℃ for one year.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e1. This product is for research use only.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e2. For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this product.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eDocuments:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eSafety Data Sheet\u003c\/p\u003e\n\u003cp\u003e10674_MSDS_HB251111_EN.PDF\u003c\/p\u003e\n\u003cp\u003eManuals\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10674ES-Manual-Ver.EN20251113.pdf?v=1763451872\"\u003e10674_Manual_Ver.EN20251113.pdf\u003c\/a\u003e\u003c\/p\u003e","brand":"Yeasen","offers":[{"title":"1 mL","offer_id":52535361274174,"sku":"10674ES03","price":25.0,"currency_code":"USD","in_stock":true},{"title":"10 mL","offer_id":52535361306942,"sku":"10674ES10","price":235.0,"currency_code":"USD","in_stock":true},{"title":"25 mL","offer_id":52535361339710,"sku":"10674ES25","price":495.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10670_7ba4ca0c-47cd-4343-a523-2c8d09c22bbc.png?v=1768375879"},{"product_id":"10636","title":"Hieff™ T7 RNA Polymerase(low dsRNA, 1000 U\/μL) _ 10636ES","description":"\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThis product is a bacteriophage T7 RNA polymerase derived from recombinant expression in E. coli. It is a T7 RNA polymerase obtained by mutation after modification and optimization of wild-type T7, which can significantly reduce the dsRNA content. Double-stranded DNA containing the T7 promoter sequence is used as a template and NTP is used as a substrate to synthesize RNA complementary to the reverse single-stranded DNA downstream of the promoter. Double-stranded linear blunt-end or 5' protruding end DNA can be used as a substrate template for T7 RNA polymerase, so linear plasmids and PCR products can be used as templates for in vitro RNA synthesis.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 align=\"justify\"\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eSpecifications\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"281\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSource\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"570\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eRecombinant\u003c\/span\u003e\u003ci\u003e\u003cspan\u003e E. coli\u003c\/span\u003e\u003c\/i\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"281\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eOptimum temperature\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"570\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e37℃\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"281\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eActivity\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"570\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e100\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e0 U\/μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"281\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eResidual RNase \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"570\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eNegative\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"281\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eStorage buffer\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"570\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003emM Tris-HCl, 1 mM EDTA, 10 mM DTT, 100 mM NaCl, 0.1% Triton X-100, 50% (v\/v) glycerol, pH7.9@25℃\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"281\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eActivity unit definition\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"570\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThe amount of enzyme required to incorporate 1 nmol of [\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eH] GMP into the acid-insoluble precipitate within 1 hour at 37℃ and pH8.0 is defined as one activity unit.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"15.5600%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"30.0800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"13.5800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e10636\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eES\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e5\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e(50 KU)\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"13.5800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e10636\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eES\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e6\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e(500 KU)\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"13.5800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e10636\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eES\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e72\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e(1 MU)\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"13.5800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e10636ES86\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e(10 MU)\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"15.5600%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e10636\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"30.0800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHieff\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eT7 RNA Polymerase (low dsRNA,\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e1000 U\/μL)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"13.5800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e5\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e0 \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"13.5800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e5\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e0\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"13.5800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e1\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003emL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"13.5800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e10 mL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eStorage\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eThis product should be stored at -25~-15℃ for 1 year.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eFigures\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e1. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eCustomer feedback\u003c\/span\u003e\u003cspan\u003e \u003cspan style=\"font-family: Inter Medium;\"\u003e1\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eExperimental Design\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e: \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable class=\"MsoNormalTable\" border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"23.2600%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003eParameter\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"75.6400%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003eCondition\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"23.2600%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eObjective\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"75.6400%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eTo compare application performance between WT T7 and Low-dsRNA T7\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"23.2600%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eReaction Volume\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"75.6400%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e20 µL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"23.2600%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eTemplate Length\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"75.6400%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e2 kb\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"23.2600%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eTest Samples\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"75.6400%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eWT T7 \u0026amp; Low-dsRNA T7\u003c\/span\u003e\u003cspan\u003e \u003cspan style=\"font-family: Inter Medium;\"\u003eRNA polymerase\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"23.2600%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eReaction Temperature\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"75.6400%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e27 °C\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"23.2600%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eReaction Time\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"75.6400%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e2 h\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eResults:\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003eTable 1. Comparison of dsRNA Content and Yield Between WT T7 and Low-dsRNA T7\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"MsoNormalTable\" border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"24.0800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003eEnzyme Type\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"30.6800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003edsRNA Content (%)\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"19.8400%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003eIntegrity (%)\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"23.5800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003eReaction Scale\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"24.0800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eWT T7\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"30.6800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e0.0030%\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"19.8400%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e92.5\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"23.5800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e180 µg (20 µL)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"24.0800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eLow-dsRNA T7\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"30.6800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e0.0003%\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"19.8400%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e93.0\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"23.5800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e180 µg (20 µL)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eResult Summary\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e: \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eLow-dsRNA T7 reduced dsRNA content by approximately 10-fold while maintaining comparable RNA yield and integrity relative to WT T7.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e2. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eCustomer feedback\u003c\/span\u003e\u003cspan\u003e \u003cspan style=\"font-family: Inter Medium;\"\u003e2\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003eTable 2. Performance Comparison Under Different GAG Final Concentrations\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003eYield (mg\/mL)\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"82\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eGAG Final Concentration (mM)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"3\" valign=\"center\" width=\"171\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eYield (mg\/mL)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"3\" valign=\"center\" width=\"190\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eIntegrity (%)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"3\" valign=\"center\" width=\"170\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eCapping Efficiency (%)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"3\" valign=\"center\" width=\"166\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eTransfection Efficiency (%)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"82\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"42\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eWT\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"61\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eYeasen\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"67\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003esupplier A*\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"66\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eWT\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"60\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eYeasen\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"63\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003esupplier A*\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"52\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eWT\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"57\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eYeasen\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"60\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003esupplier A*\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"45\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eWT\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"60\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eYeasen\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"61\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003esupplier A*\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"82\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"42\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e12.14\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"61\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e11.376\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"67\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e12.103\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"66\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e88.1\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"60\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e92.5\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"63\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e92.3\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"52\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e96.7\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"57\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"60\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"45\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e44.60\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"60\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e33.63\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"61\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e30.76\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"82\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"42\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e11.82\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"61\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10.716\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"67\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e11.337\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"66\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e82.2\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"60\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e91.1\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"63\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e91.5\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"52\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e98.3\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"57\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"60\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"45\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e42.30\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"60\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e32.78\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"61\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e32.37\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"82\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e2.5\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"42\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e11.31\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"61\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10.716\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"67\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e11.066\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"66\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e82.9\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"60\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e91.7\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"63\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e91.8\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"52\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e98.8\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"57\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e97.3\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"60\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e97.8\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"45\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e39.78\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"60\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e31.68\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"61\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e32.59\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"82\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1.5\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"42\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e11.98\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"61\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"67\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"66\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e78.9\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"60\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"63\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"52\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e97.7\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"57\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"60\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"45\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"60\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"61\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"82\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"42\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e11.75\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"61\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"67\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"66\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e80.0\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"60\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"63\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"52\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e90.5\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"57\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"60\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"45\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"60\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"61\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"82\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e0.5\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"42\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e11.65\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"61\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"67\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"66\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e80.9\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"60\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"63\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"52\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e70.1\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"57\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"60\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"45\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"60\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"61\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eResult Summary\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e:\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003eEffects of cap analog concentration on IVT performance. Reactions were carried out in a 20 µL system at 27 °C for 2 h using different T7 enzymes. RNA yield remained stable as cap analog input decreased, while integrity and capping efficiency showed a gradual decline. A cap analog concentration of ≥2.5 mM is recommended for optimal performance.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\" align=\"justify\"\u003e\n\u003cb\u003e\u003cspan\u003eDocuments:\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSafety Data Sheet\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10636ES-MSDS-HB260115.pdf?v=1768791291\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e10636\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e_MSDS_HB\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e60115\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eManuals\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10636ES-Manual-Ver.EN20251208.pdf?v=1768791291\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e10636\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e_Manual_Ver.EN20251208\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Yeasen","offers":[{"title":"50 KU","offer_id":52897427915070,"sku":"10636ES50","price":765.0,"currency_code":"USD","in_stock":true},{"title":"500 KU","offer_id":52897427947838,"sku":"10636ES60","price":5365.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10636_9392514d-1c2b-481e-8f0e-832980d42522.png?v=1772006901"},{"product_id":"14619","title":"Tko RNA Helicase (100 µg\/mL) _ 14619ES","description":"\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eTko RNA Helicase is derived from the TK0306 gene of the thermophilic archaeon Thermococcus kodakaraensis (strain KOD1) and belongs to the DEAD-box RNA helicase family. This enzyme is ATP-dependent and specifically unwinds single-stranded stem-loop RNA structures. It exhibits weak activity towards double-stranded RNA (dsRNA) and single-stranded DNA (ssDNA) substrates and does not act on dsRNA substrates with 5' or 3' overhangs. With an optimal temperature of 50°C, this enzyme serves as a useful tool for specifically unwinding RNA stem-loop structures under mild temperature conditions, making it suitable for applications such as RNA structural analysis and post-transcriptional regulation research.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eSpecifications\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"311\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eCat.No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"651\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e14619ES08\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\/ 14619ES20\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"311\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eSize\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"651\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e5 μg\u003c\/span\u003e\u003cspan\u003e\/ \u003c\/span\u003e\u003cspan\u003e20 μg\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"311\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eReaction Temperature\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"651\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e50℃\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"311\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eInactivation Conditions\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"651\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eIncubate at 80°C for 5 minutes\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"20.5400%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"42.4600%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"182\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e14619ES08\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"182\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e14619ES20\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"202\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e14619\u003c\/span\u003e\u003cspan\u003e-A\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"418\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eTko RNA Helicase (100 µg\/mL)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"182\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e50 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"182\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e200 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"202\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e14619\u003c\/span\u003e\u003cspan\u003e-B\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"418\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e10×Tko Reaction Buffer\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"182\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"182\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e1.5 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThis product should be stored at -25~-15℃ for \u003c\/span\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e year.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eInstructions\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1. \u003c\/span\u003e\u003cspan\u003ePrepare the following reaction mixture on ice\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"57.3600%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eComponent\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"42.6200%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eVolume(μL\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e）\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"57.3600%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e10×Tko Reaction Buffer\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"42.6200%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"57.3600%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003essRNA (5–10 nM)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"42.6200%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"57.3600%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eTko RNA Helicase (100 µg\/mL)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"42.6200%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e0.5–1\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"57.3600%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eMurine RNase inhibitor(40 U\/μL)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"42.6200%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e0.1\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"57.3600%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eDEPC H\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003eO\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"42.6200%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eUp to 20 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e2. Incubate at 50°C for 30 min\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eNotes\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e1. This product is for research use only.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e2. Please operate with lab coats and disposable gloves\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e，\u003c\/span\u003e\u003cspan style=\"font-family: Inter;\"\u003efor your safety.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e3. \u003c\/span\u003e\u003cspan\u003eThe reaction buffer for this enzyme requires 1–2 mM ATP or dATP and 2 mM Mg²⁺. The 10×Tko Reaction Buffer already contains ATP and Mg²⁺.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e4. \u003c\/span\u003e\u003cspan\u003eFor different substrates, the enzyme amount should be adjusted; gradient testing is recommended.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eDocuments\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/14619-MSDS-HB260312.pdf?v=1773393695\"\u003e\u003cspan\u003e14619_MSDS_HB260312\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/14619-Manual-Ver.EN20260304.pdf?v=1773393695\"\u003e\u003cspan\u003e14619_Manual_Ver.EN20260304\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Yeasen","offers":[{"title":"5 μg","offer_id":53143030825278,"sku":"14619ES08","price":125.0,"currency_code":"USD","in_stock":true},{"title":"20 μg","offer_id":53143030858046,"sku":"14619ES20","price":375.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/universe_b9e9ebcb-b5e9-497a-b61d-dc198ade0f05.jpg?v=1754453675"}],"url":"https:\/\/www.yeasenbio.com\/ar\/collections\/mrna-enzyme.oembed","provider":"Yeasen - Leading Innovation in Molecular Enzymes and Reagents","version":"1.0","type":"link"}