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Recombinant Human Dickkopf-related protein-1,His 92275ES
物品单位 品牌
Yeasen
  • 型号:20 μg
  • 货号:92275ES20
  • 发布日期: 2022-04-28
  • 更新日期: 2022-04-29
产品详细说明
产地
品牌 Yeasen
货号 92275ES20
用途范围
英文名称
纯度 %
CAS编号
规格 20 μg
保质期
是否进口

Product Information

Product Name

Cat#

Size

Price(

Recombinant Human Dickkopf-related protein-1,His (Human DKK-1,His )

92275ES20

20 μg

2,675

92275ES60

100 μg

7,985

92275ES80

1 mg

29,655

Product Description

Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Each domain contains ten cysteine residues . Mature human Dkk-1 is a 40 kDa glycosylated protein that shares 86%, 87%, 90% and 91% aa sequence identity with mouse, rat, rabbit and bovine Dkk-1, respectively. It also shares 42% and 36% aa identity with human Dkk-2 and Dkk-4, respectively. Dkk-1 and Dkk-4 are well documented antagonists of the canonical Wnt signaling pathway. This pathway is activated by Wnt engagement of a receptor complex composed of the Frizzled proteins and one of two low-density lipoprotein receptor-related proteins, LRP5 or LRP6 . Dkk-1 antagonizes Wnt by forming ternary complexes of LRP5/6 with Kremen1 or Kremen2. Dkk1/LRP6/Krm2 complex internalization has been shown to down-regulate Wnt signaling. Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development. Other sites of expression include developing neurons, hair follicles and the retina of the eye .

Product Properties

Synonyms

Dickkopf-1;DKK-1;SK Protein; DKK1; dkk1; dkk-1

Accession

O94907

Source

HEK293 Cells-derived human Dkk-1 protein, Met2-His 266 with His tag at the C-terminus.

Molecular Weight

Approximately 27.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh DKK1 is approximately 42.9 kDa due to glycosylation.

AA Sequence

MALGAAGAT RVFVAMVAAA LGGHPLLGVS ATLNSVLNSN AIKNLPPPLG GAAGHPGSAV SAAPGILYPG GNKYQTIDNY QPYPCAEDEE CGTDEYCASP TRGGDAGVQI CLACRKRRKR CMRHAMCCPG NYCKNGICVS SDQNHFRGEI EETITESFGN DHSTLDGYSR RTTLSSKMYH TKGQEGSVCL RSSDCASGLC CARHFWSKIC KPVLKEGQVC TKHRRKGSHG LEIFQRCYCG EGLSCRIQKD HHQASNSSRL HTCQRH

Tag

His

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 90% by SDS-PAGE.

Biological Activity

Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The ED50 for this effect is approximately 0.1-0.4 μg/mL in the presence of 10 ng/mL of mouse Wnt3a.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.


Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

Shipping and Storage

The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

Cautions

1. Avoid repeated freeze-thaw cycles.

2. For your safety and health, please wear lab coats and disposable gloves for operation.

3. For research use only.